A SNP molecular marker associated with growth traits of Pelteobagrus vachelli and application thereof

A technique for the growth traits of peltachlox vacheronii, applied in the field of aquatic animal genetics and molecular marker-assisted selection breeding, can solve the problems of wild resources and germplasm decline, seedling quality varies, and head length/body length increase, etc. Achieve accurate and reliable identification results, promote healthy development, and promote the effect of breeding progress

Active Publication Date: 2019-01-11
HEZE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the rapid expansion of the scale of breeding and breeding of Pelvis vacheronii, there are many breeding problems, such as the degradation of economic traits: the original method of fishing wild parents for breeding and seed production has led to a serious decline in wild resources and germplasm, and the trend of miniaturization has intensified. or multiple generations of close relatives for seedling production, the quality of the produced seedlings varies, such as slow growth, increased head length / body length, and impure color of commercial fish

Method used

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  • A SNP molecular marker associated with growth traits of Pelteobagrus vachelli and application thereof
  • A SNP molecular marker associated with growth traits of Pelteobagrus vachelli and application thereof
  • A SNP molecular marker associated with growth traits of Pelteobagrus vachelli and application thereof

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Embodiment 1

[0026] The SNP molecular marker of the present invention is derived from the genetic map of the F1 progeny constructed by our laboratory of Peeled catfish, and then obtained through the QTL mapping of growth traits (body weight, body width, and full length):

[0027] (1) Mining SNPs molecular markers and constructing a high-density genetic linkage map, using parents from different geographical groups and segregated traits, establishing the F1 generation family of Peltedelachus vacherii for > 3 months under the same conditions, and using microsatellite markers to screen A family with a high degree of heterozygosity was selected, and 200 full-sibling F1 generations and their parents were randomly selected as a mapping group, and the growth traits (weight, body width, total length, etc.) The -seq technology mines SNPs molecular markers and constructs a high-density genetic linkage map (Joinmap) of Pediatric catfish.

[0028] (2) QTL mapping for growth traits (body weight, body wi...

Embodiment 2

[0029] Example 2 Detection of the SNP molecular marker of the sample

[0030]Since the film was released in late July, 2017, 450 individual pelturotus vacheronii were cultured for 105 days under the same environment (temperature, density, etc.); the 450 fish were placed in a pool (580L) to adapt for 2 weeks, and carried out Stop feeding the feed for 2 days before the experiment, use a caliper to measure its body width and total length, and use an electronic balance to measure its body weight; randomly select 193 Pelvis catfish, cut their tail fins and store them in 95% ethanol at -20°C for use in Genomic DNA extraction, the specific steps are as follows:

[0031] (1) Take a small amount of caudal fins and extract the genomic DNA of Peeled catfish;

[0032] (2) PCR amplification is carried out by using a specific primer set, and the resulting amplification product is scanned for fluorescent signals;

[0033] (3) Determine whether the genotype of the SNP site at the 26bp posit...

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Abstract

The present invention discloses a SNP molecular marker associated with growth traits of Pelteobagrus vachelli, which is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO: 1, and a SNP site exists at the 26th position of the gene sequence: the base is G or C. The SNP molecular marker is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The invention also discloses a specific primer, a kit and a detection method for detecting the molecular marker. It is found that, The genotype ofthe SNP site at 26 bp of the gene at 41 cM in linkage group 14 of Pelteobagrus vachelli is GG, GC or CC is closely related to growth traits of Pelteobagrus vachelli, such as body weight, body width and total length. Therefore, the SNP molecular marker of the invention can be used for detecting or assisting in detecting growth traits of Pelteobagrus vachelli, and can be used for assisting selectionand breeding of Pelteobagrus vachelli. The invention has important guiding significance for breeding new strain of excellent growth characteristics of Pelteobagrus vachelli.

Description

technical field [0001] The invention relates to a SNP molecular marker related to the growth traits of Pelvic albus varschii and its application, and belongs to the technical field of aquatic animal genetics and molecular marker-assisted selection breeding. Background technique [0002] Pelteobagrus vachelli is the largest species in the genus Pelteobagrus (body weight up to 1800g), and is the two most economically valuable species of the genus together with P.fulvidraco At present, the new fish strain "Huangyou No. 1" with high breeding popularity is obtained by crossing the yellow catfish as the male parent and the yellow catfish as the female parent. It has fast growth, attractive body color, and disease resistance. Strength and other advantages. In the cultivation of new aquatic species, the most important link is the selection of parents, and the current new aquatic strain "Huangyou No. Fish, the research on germplasm improvement obviously lags behind, and there are f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张国松楚德昌朱道玉孙西寨张贵生张红梅梁霞
Owner HEZE UNIV
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