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Storing method of pollens subjected to soft x ray irradiation

An X-ray and pollen technology, applied in the field of seedless watermelon cultivation, can solve the problem of low fruit setting rate, achieve long storage time and maintain the effect of pollen activity

Inactive Publication Date: 2015-09-02
桂林嘉佰农农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is stated in the article that the fruit setting rate is generally low due to the low temperature encountered during pollination, the fruit setting rate is still low

Method used

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  • Storing method of pollens subjected to soft x ray irradiation
  • Storing method of pollens subjected to soft x ray irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Obtain diploid male flower pollen according to the existing conventional technology, place the obtained pollen in a watch glass, and then place it in a soft X-ray irradiation device commonly used in the prior art, set the irradiation dose to 800GY, and the irradiation time to 60min , to obtain the irradiated pollen;

[0023] 2) Put the irradiated pollen in an aluminum foil bag, then evacuate the aluminum foil bag to a relative vacuum of 0.6Kpa, then fill the aluminum foil bag with nitrogen until the pressure is 61Kpa, seal the aluminum foil bag, and place it at -5°C Store under conditions.

Embodiment 2

[0028] 1) Pick the male flowers that open on the same day at 8:30-10:30 in the morning, collect the anthers, then place the collected anthers in a watch glass, spread them to a thickness of 2mm, and store them in a room at 26°C and a relative humidity of 60-80%. Dry in the environment for 60 minutes, then remove impurities (insects, petals, small stones, etc.) to obtain diploid male flower pollen; place the pollen in a watch glass, and then place it in a soft X-ray irradiation device, and set the irradiation dose to 800GY , the irradiation time is 60min, and the pollen after irradiation is obtained;

[0029] 2) Put the irradiated pollen in an aluminum foil bag, then evacuate the aluminum foil bag to a relative vacuum of 0.5Kpa, then fill the aluminum foil bag with nitrogen until the pressure is 65Kpa, seal the aluminum foil bag, and place it at -15°C Store under conditions.

Embodiment 3

[0034] 1) Obtain diploid male flower pollen according to the existing conventional technology, place the obtained pollen in a watch glass, and then place it in a soft X-ray irradiation device commonly used in the prior art, set the irradiation dose to 800GY, and the irradiation time to 50min , to obtain the irradiated pollen;

[0035] 2) The obtained irradiated pollen is placed in a plastic ziplock bag, then the plastic ziplock bag is evacuated to a relative vacuum of 0.3Kpa, and then helium is filled into the aluminum foil bag until the pressure is 20Kpa, the aluminum foil bag is sealed, and placed in Store at -10°C.

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Abstract

The invention discloses a storing method of pollens subjected to soft x ray irradiation. The storing method particularly comprises the following steps: subpacking the pollens subjected to soft x ray irradiation in a packing bag or a vessel, then vacuumizing the packing bag or the vessel, filling the packing bag or the vessel with inert gas, and storing at the temperature of lower than or equal to -5 DEG C. The pollens subjected to soft x ray irradiation, which is stored according to the storing method, are long in storing time, and after the pollens are stored for 1 year, the activity of the pollens is more than 75% of that of pollens which are not stored. The applicant experiment shows that even if the pollens after being stored for 1 year are subjected to pollinating and induced parthenocarpy according to the existing conventional technology, seedless watermelons can be still obtained, wherein the fruit setting rate is more than 75%, and the occupancy of the seedless watermelons in the obtained commodity fruit is more than 80%.

Description

technical field [0001] The invention relates to a method for cultivating seedless watermelons, in particular to a method for storing pollen irradiated by soft X-rays. Background technique [0002] Watermelon (Citrullus lanatus), also known as water melon and cold melon, is a species of watermelon in the family Cucurbitaceae. The fruit flesh developed by fetal sitting is used as the product, which is rich in various nutrients needed by the human body. Every 100 grams of watermelon pulp contains about 1.3 grams of protein, 4.2 grams of carbohydrates, 0.3 grams of crude fiber, 6 mg of calcium, 10 mg of phosphorus, 0.2 mg of iron, 0.17 mg of provitamin A (carotene), 0.02 mg of vitamin B1, and 0.02 mg of B2, 3 mg of vitamin C, 92.1 kJ of heat, and various amino acids, organic acids, inorganic salts and trace elements such as zinc and potassium. Contains sugars including sucrose, fructose and glucose. The flesh of watermelon is crisp and tender, sweet and juicy, rich in mineral...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 林智明
Owner 桂林嘉佰农农业科技有限公司
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