Preserving fluid and preserving method for maintaining activity of amniotic mesenchymal stem cells

Abstract

The invention discloses a preserving fluid for maintaining the activity of amniotic mesenchymal stem cells. The preserving fluid is prepared from, by weight, 90 to 100 parts of sodium chloride solution with the mass concentration of 0.5 to 0.9 percent, 1 to 5 parts of mung bean protein, 0.5 to 1 part of endorphin, 0.1 to 0.5 part of sulforaphane, 5 to 10 parts of L-theanine, 2 to 8 parts of L-phenylalanine and 5 to 10 parts of L-tryptophan. The mung bean protein, the enorphin and the sulforaphane are added into the preserving fluid provided by the invention to synergistically provide a protection effect for cells and jointly play a role with the amino acid for providing energy for the cells and the sodium chloride solution for maintaining extracellular osmotic pressure in the preserving fluid, and the motility rate of the cells after being preserved at 4-10 DEG C for 48 hours is greater than 90%; and the preserving fluid has clear components and is safe to use. The invention further provides a preserving method for the amniotic mesenchymal stem cells. The preserving fluid is adopted to preserve the amniotic mesenchymal stem cells obtained through subculture, the preservation time of the cells is effectively prolonged, and a guarantee is provided for long-distance transportation of the amniotic mesenchymal stem cells.

Description

technical field [0001] The invention relates to a preservation solution, in particular to a preservation solution and a preservation method for maintaining the activity of amniotic mesenchymal stem cells. Background technique [0002] Human amniotic mesenchymal stem cells have high self-renewal, proliferation, implantability and multilineage differentiation capabilities, and there is no risk of immune rejection and tumorigenicity after transplantation in allogeneic individuals. Moreover, human amniotic mesenchymal stem cells are derived from the discarded placenta of puerpera after childbirth, and their clinical application will not bring about medical ethics controversy. Amniotic mesenchymal stem cells can be used for cell therapy of many diseases, repair and reconstruction of tissue and organ damage, are ideal seed cell resources in the field of regenerative medicine, and have broad clinical application prospects. [0003] The treatment of amniotic mesenchymal stem cells ...

AI Technical Summary

Problems solved by technology

If human amniotic mesenchymal stem cells are isolated, cultured, and expanded, and immediately applied to patients, normal saline is convenient and safe, but the viability of amniotic mesenchymal stem cells declines very quickly in normal saline, so they should be preserved with normal saline alone. The cell viability will decrease by about 10% after 2 hours, so it is only suitable for immediate use of cells

[0004] Exogenous substances are added to the existing cell preservation solution to increase the storage time, which will affect its safety. At the same time, the time for the existing cell preservation solution to maintain cell viability cannot meet the needs of use

In the cell preservation solution of the prior art, more than 90% of the viability can be maintained for only 24 hours, and as time goes on, the viability of the cells decreases, which means that the viability of the cells will drop below 90% after 24 hours, but the amnion stem cells Treatment and clinical trials have been carried out in most cities in China and many foreign countries. Using the existing means of transportation, the 24-hour vitality maintenance time obviously limits the application range of amniotic mesenchymal stem cells

Images