Polymer material entrapping glucose oxidase and preparation method and application of polymer material

A technology of glucose oxidase and polymer materials, which is applied in the field of polymer materials carrying glucose oxidase and its preparation, can solve the problems of difficulty in effectively exerting curative effect, poor selectivity, and poor stability, and achieve excellent biocompatibility , maintaining the activity of biological enzymes, and the effect of high-efficiency delivery

Pending Publication Date: 2020-11-20
HUNAN PROVINCIAL TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a polymer material carrying glucose oxidase and its preparation method and application, the purpose of which is to overcome the disadvantages of poor stab

Method used

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  • Polymer material entrapping glucose oxidase and preparation method and application of polymer material
  • Polymer material entrapping glucose oxidase and preparation method and application of polymer material
  • Polymer material entrapping glucose oxidase and preparation method and application of polymer material

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Example Embodiment

[0045] Example 1

[0046] A polymer carrier with enzyme protection, including folic acid and zinc chloride to form a ligand template. The polymer of this embodiment was prepared by the following method:

[0047] (1) Take 2mL of 2mM folic acid (FA) into a 10mL beaker, add 1mL of 2wt% polyvinyl alcohol (PVA), then add 50μL of glucose oxidase (GOx), add 500mL of zinc chloride under stirring at room temperature, The final volume of the system is 4 mL, and the mixed solution is obtained by sonicating for 5 min.

[0048] (2) Add 400mL of 0.1M Tris solution (pH=8.0) to the solution obtained in step (1), adjust the pH to about 7.6, and then take 400μL of 5mg / mL dopamine (DA) into the mixed solution Stir for 48 hours, centrifuge, and reconstitute the enzyme-containing metal framework polydopamine nanoparticles, denoted as NCPs / GOx@PDA NPs.

Example Embodiment

[0049] Example 2

[0050] A polymer carrier with enzyme protection function includes dissociating ligand templates to obtain polydopamine nanoparticles. The polymer of this embodiment was prepared by the following method:

[0051] (1) Take 2mL of 2mM folic acid (FA) into a 10mL beaker, add 1mL of 2wt% polyvinyl alcohol (PVA), then add 50μL of glucose oxidase (GOx), add 500mL of zinc chloride under stirring at room temperature, The final volume of the system is 4 mL, and the mixed solution is obtained by ultrasonic for 5 min.

[0052] (2) Add 400mL of 0.1M Tris solution (pH=8.0) to the solution obtained in step (1), adjust the pH to about 7.6, and then take 400μL of 5mg / mL dopamine (DA) into the mixed solution Stir for 48 hours, after centrifugation, dialysis with a concentration of 10mM phosphate buffer (pH=7.4) for 1 hour, centrifugation and reconstitution to obtain GOx@PDA NPs.

Example Embodiment

[0053] Example 3

[0054] A polymer carrier with enzyme protection function includes dissociating ligand templates to obtain polydopamine nanoparticles. The polymer of this embodiment was prepared by the following method:

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Abstract

The invention provides a polymer material entrapping glucose oxidase and a preparation method and application of the polymer material. The polymer material is a polydopamine nanocage composed of polydopamine. According to the polymer material, first a nano nano-coordination polymer is formed through folic acid-zinc chloride coordination to entrap glucose oxidase, then dopamine is oxidized and self-polymerized on the surface of the nano-coordination polymer to form a polydopamine shell, and finally the nano-coordination polymer is dissociated to obtain the polymer material. The invention provides a self-polymerizing nano cage carrier, the preparation steps are simple, the carrier with stable particle size, capable of effectively maintaining the activity of biological enzymes under physiological conditions and delivering protein drugs is obtained, and the high-efficiency delivery is achieved and the biological active is maintained.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a polymer material carrying glucose oxidase and its preparation method and application. Background technique [0002] Glucose oxidase (GOx) is an endogenous oxidoreductase widely present in organisms. Due to its biocompatibility, non-toxicity and unique catalytic effect on glucose, it has attracted more and more attention in the field of biomedicine. GOx can effectively catalyze the oxidation of glucose to gluconic acid and hydrogen peroxide, based on this catalytic effect, various therapeutic strategies have also been developed: (1) glucose depletion provides an alternative strategy for tumor starvation therapy; (2) oxygen depletion Increased tumor hypoxia, which can be used to activate therapy; (3) Gluconic acid production enhances the acidity of the tumor microenvironment, which can trigger pH-responsive drug release; (4) H 2 o 2 The generation of increased tumor oxidative...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K9/51A61K47/34A61K47/69C08G73/06A61P35/00
CPCA61K9/5146A61K38/443A61K47/6949A61P35/00C08G73/0672C12Y101/03004
Inventor 刘淼曾杨周文虎
Owner HUNAN PROVINCIAL TUMOR HOSPITAL
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