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Polymer material entrapping glucose oxidase and preparation method and application of polymer material

A technology of glucose oxidase and polymer materials, which is applied in the field of polymer materials carrying glucose oxidase and its preparation, can solve the problems of difficulty in effectively exerting curative effect, poor selectivity, and poor stability, and achieve excellent biocompatibility , maintaining the activity of biological enzymes, and the effect of high-efficiency delivery

Pending Publication Date: 2020-11-20
HUNAN PROVINCIAL TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a polymer material carrying glucose oxidase and its preparation method and application, the purpose of which is to overcome the disadvantages of poor stability, poor selectivity and easy inactivation in the process of delivering protein substances such as glucose oxidase. The problem that it is difficult to effectively exert its curative effect

Method used

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  • Polymer material entrapping glucose oxidase and preparation method and application of polymer material
  • Polymer material entrapping glucose oxidase and preparation method and application of polymer material
  • Polymer material entrapping glucose oxidase and preparation method and application of polymer material

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Embodiment 1

[0046] A polymer carrier with enzyme protection, including folic acid and zinc chloride to form a complex template. The polymer of this embodiment is prepared by the following method:

[0047] (1) Get 2mL concentration of 2mM folic acid (FA) into a 10mL beaker, add 1mL concentration of 2wt% polyvinyl alcohol (PVA), then throw 50 μL glucose oxidase (GOx), add 500mL zinc chloride under stirring at room temperature, The final volume of the system was 4 mL, and the mixed solution was obtained by ultrasonication for 5 min.

[0048](2) Add 400 mL of Tris solution (pH=8.0) with a concentration of 0.1M to the solution obtained in step (1), adjust the pH value to about 7.6, and then add 400 μL of dopamine (DA) with a concentration of 5 mg / mL to the mixed solution Stir for 48 hours, centrifuge, and redissolve to obtain polydopamine nanoparticles with metal frameworks loaded with enzymes, which are denoted as NCPs / GOx@PDA NPs.

Embodiment 2

[0050] A polymer carrier with enzyme protection function, including dissociating a ligand template to obtain polydopamine nanoparticles. The polymer of this embodiment is prepared by the following method:

[0051] (1) Get 2mL concentration of 2mM folic acid (FA) into a 10mL beaker, add 1mL concentration of 2wt% polyvinyl alcohol (PVA), then drop into 50 μL glucose oxidase (GOx), add 500mL zinc chloride under stirring at room temperature, The final volume of the system was 4 mL, and the mixed solution was obtained by ultrasonication for 5 min.

[0052] (2) Add 400 mL of Tris solution (pH=8.0) with a concentration of 0.1M to the solution obtained in step (1), adjust the pH value to about 7.6, and then add 400 μL of dopamine (DA) with a concentration of 5 mg / mL to the mixed solution Stir for 48 hours, centrifuge and dialyze with 10 mM phosphate buffer (pH=7.4) for 1 hour, centrifuge and redissolve to obtain GOx@PDA NPs.

Embodiment 3

[0054] A polymer carrier with enzyme protection function, including dissociating a ligand template to obtain polydopamine nanoparticles. The polymer of this embodiment is prepared by the following method:

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Abstract

The invention provides a polymer material entrapping glucose oxidase and a preparation method and application of the polymer material. The polymer material is a polydopamine nanocage composed of polydopamine. According to the polymer material, first a nano nano-coordination polymer is formed through folic acid-zinc chloride coordination to entrap glucose oxidase, then dopamine is oxidized and self-polymerized on the surface of the nano-coordination polymer to form a polydopamine shell, and finally the nano-coordination polymer is dissociated to obtain the polymer material. The invention provides a self-polymerizing nano cage carrier, the preparation steps are simple, the carrier with stable particle size, capable of effectively maintaining the activity of biological enzymes under physiological conditions and delivering protein drugs is obtained, and the high-efficiency delivery is achieved and the biological active is maintained.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a polymer material carrying glucose oxidase and its preparation method and application. Background technique [0002] Glucose oxidase (GOx) is an endogenous oxidoreductase widely present in organisms. Due to its biocompatibility, non-toxicity and unique catalytic effect on glucose, it has attracted more and more attention in the field of biomedicine. GOx can effectively catalyze the oxidation of glucose to gluconic acid and hydrogen peroxide, based on this catalytic effect, various therapeutic strategies have also been developed: (1) glucose depletion provides an alternative strategy for tumor starvation therapy; (2) oxygen depletion Increased tumor hypoxia, which can be used to activate therapy; (3) Gluconic acid production enhances the acidity of the tumor microenvironment, which can trigger pH-responsive drug release; (4) H 2 o 2 The generation of increased tumor oxidative...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K9/51A61K47/34A61K47/69C08G73/06A61P35/00
CPCA61K9/5146A61K38/443A61K47/6949A61P35/00C08G73/0672C12Y101/03004
Inventor 刘淼曾杨周文虎
Owner HUNAN PROVINCIAL TUMOR HOSPITAL
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