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Application of tomato growth hormone receptor homologous gene S1TIR1 in fruit parthenocarpy

A homologous gene, parthenocarpic technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as affecting crop yield and crop failure to pollinate successfully.

Inactive Publication Date: 2012-07-04
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under natural conditions, there are many factors that affect the pollination process. For example, if the temperature is too high or too low, the crops will not be pollinated successfully, which will affect the crop yield.
However, there is no report on the effect of the tomato auxin receptor homolog gene SlTIR1 on parthenocarpy

Method used

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  • Application of tomato growth hormone receptor homologous gene S1TIR1 in fruit parthenocarpy
  • Application of tomato growth hormone receptor homologous gene S1TIR1 in fruit parthenocarpy
  • Application of tomato growth hormone receptor homologous gene S1TIR1 in fruit parthenocarpy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: tomato SlTIR1 gene cDNA clone

[0027] The comparison and analysis of the tomato EST database showed that the EST sequence (SGN-U327259) had a high homology with the Arabidopsis AtTIR1 sequence. The comparison of the nucleotide sequences of SGN-U327259 and AtTIR1 showed that the EST already contained the 5' end DNA sequence. Therefore, this EST was selected for cloning the auxin receptor homologous gene cDNA. According to the EST sequence, we designed a sequence-specific primer P1: 5'-GAAGCCTCATTTTGCTGATTTCA-3' (SEQ ID NO: 4). Total RNA was extracted from tomato (Micro-Tom) seedlings using the TRIZOL (invitrogen, USA) method, and 2 μg of total RNA was used to prepare cDNA using the Fermentas Reverse Transcription Kit (Fermentas, UK). The entire operation was performed according to the kit instructions.

[0028] According to the instructions of the 3’-Full Race Core kit, the P1 primer and the other three primers in the kit were used to amplify the cDNA 3’...

Embodiment 2

[0029] Embodiment 2: Obtaining of the complete sequence of tomato SlTIR1 gene

[0030] The obtained cDNA sequence was compared with the tomato database (SGN) to obtain the complete sequence of the tomato SlTIR1 gene. The gene consists of 3849 deoxyribonucleotides (see sequence 1 in the sequence listing). The SlTIR1 genome starts from the 5' start codon ATG to the 3' stop codon TAG, with a total length of 3849 nucleotides, including 3 exons and 2 introns. The structure of the SlTIR1 gene (see figure 1 ).

Embodiment 3

[0031] Embodiment 3: Expression analysis of SlTIR1 gene in different tissues of plants

[0032] Materials from different tissues of tomato (root, stem, leaf, flower, immature green fruit, ripe green fruit, color-breaking fruit, ripe fruit) were used for semi-quantitative analysis. The analysis results showed that SlTIR1 was highly expressed in flowers, indicating that SlTIR1 may play an important role in the development of floral organs. In order to further study the role of SlTIR1 in the development of floral organs, we used materials from different parts of floral organs at different stages (sepals, stamens, pistils, and ovary 1 day before flowering; sepals, stamens, pistil, and ovary before flowering; 4 days after flowering). days) for quantitative analysis. Quantitative PCR results showed that the expression level of SlTIR1 increased in sepals and decreased significantly in stamens from before flowering to flowering stage. From the flowering period to 4 days after flower...

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Abstract

The invention discloses a tomato growth hormone receptor homologous gene S1TIR1. The gene has a nucleotide sequence as shown in SEQ ID NO:1; and transgenic crops obtained by transformation with the cDNA (complementary deoxyribonucleic acid) encoding frame sequence of the gene can generate parthenocarpy fruits. The invention also discloses DNA plasma which contains the tomato growth hormone receptor S1TIR1 gene or an ultra-expression frame constructed by the encoding frame sequence in the S1TIR1 gene. According to the invention, the encoding sequence of the gene is led into plant tissues, cells or organs through a recombinant expression vector and then transformed plant cells, tissues or organs are cultured to a plant, thus a parthenocarpy transgenic plant is obtained. The gene disclosed by the invention has important theoretical and actual significance to research on a parthenocarpy mechanism, improvement of plant parthenocarpy and modification of related characters of a plant, and has good application prospect in modification work of crops.

Description

technical field [0001] The invention relates to the application of a plant auxin receptor homologous protein SlTIR1 and its coding gene. Background technique [0002] Seedless fruit means that there are no seeds or only aborted seeds in the fruit. According to whether it is fertilized or not, it can be divided into two categories: one is parthenocarpy, and the ovary develops into fruit without pollination, which is called natural parthenocarpy or spontaneous parthenocarpy. Parthenocarpy; although pollinated, but not fertilized, or directly stimulated by other substances, the fruit developed is called stimulating parthenocarpy or artificial parthenocarpy. Another type of seedless fruit is that after the ovary is fertilized, the ovules are aborted during the development process, and there are no seeds in the grown fruit. Seedless fruit has many advantages: First, the fruit has high quality and good taste. Studies have shown that seedless tomatoes are higher in dry matter tha...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N5/10C12N15/82
Inventor 李正国任振新邓伟杨迎伍
Owner CHONGQING UNIV
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