Parthenocarpy breeding method of industrial hemp and application of parthenocarpy breeding method

An industrial hemp, parthenocarpic technology, applied in the field of breeding, which can solve problems such as lack of industrial hemp varieties

Inactive Publication Date: 2021-05-28
北大荒垦丰种业股份有限公司
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Problems solved by technology

[0010] Many places in China have traditional cultivation and production of hemp for fiber. However, due to the long-term prohibition of the cultivation and production of medicinal hemp, Yunnan and Heilongjiang provinces have not allowed the cultivation of industrial hemp under strict supervision until recent

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  • Parthenocarpy breeding method of industrial hemp and application of parthenocarpy breeding method
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  • Parthenocarpy breeding method of industrial hemp and application of parthenocarpy breeding method

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Embodiment Construction

[0054]The following will be appreciated by the following examples, and the following examples are intended to illustrate the invention.

[0055]The instruments, equipment and agents used in the examples can be obtained by various channels, such as purchasing, or can be prepared.

[0056]Early identification of marijuana female strains: uses the specific SCAR molecular molecular markers of the cannabis strains, by simple PCR analysis, can quickly and effectively identify cannabis female strains.

[0057]Sampling seedlings or unflowing plants, using a DNA rapid extraction method based on SDS (SODIUM DODECYL SULPHATE) to extract genomic DNA, and the specific SCAR tag PCR detection identification of the cannabis strain is performed (MandolinoTal., 1999). The PCR reaction system uses the Quick TAQ HS DYEMIX (DTM-101) kit (DTM-101) kit, 20 microliters of PCR reaction system comprising: 10 μL 2X Quick TAQ HS Dyemix, 1.0 μL 10 pmol / μL primer MADC2-F (gtgacgtaggatagtgaa (SEQ ID NO: 1)) or MADC5-F (ga...

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Abstract

The invention relates to the technical field of breeding, in particular to a parthenocarpy breeding method of industrial hemp and application of the parthenocarpy breeding method. The breeding method of the industrial hemp comprises the following steps: firstly inducing female plants to generate male flowers, carrying out cross seed set on the female plants of different varieties of hemp once, and mixing genetic materials of two parents; and then screening female single plants integrating excellent characters of the two parents in the filial generation, inducing male flowers to be subjected to selfing or backcross fruiting, and then repeatedly conducting multi-generation screening and selfing or backcross till the hemp variety uniform in character and stable in heredity is obtained. According to the method, a key technology for inducing female hemp plants to generate fertile male flowers and disperse pollen is developed, normal seeds capable of germinating are generated after unisexual selfing of the female plants, female plant selfing breeding and seed reproduction of the hemp are achieved, all progenies are female plants, all genetic materials of single-plant maternal female plants are kept, and the bottleneck problems of variety shortage and difficult breeding in industrial hemp production are fundamentally solved.

Description

Technical field[0001]The present invention relates to the field of breeding, in particular, to a single-sex breeding method of industrial hemp and its application.[0002]technical background[0003]Cannabis contains approximately 60 molecular structures, which are mainly synthesized by female floral leaves (Meng et al., 2018; Spitzer-Rimon et al., 2019), including tetrahydroaja THC And the chemical structure of cannabin phenol CBD is similar to the neural medium responsible for information transmission in the human brain, so there is a unique effect on people's nervous system, have a variety of common diseases such as pain, depression. Tetrahydroacetic thiol THC is the mental active ingredient of marijuana. It is the coordination of two neurocodies of human brain, which can affect mood, appetite, memory, inflammation, etc., but will happen, addictive, in multi-country still belong to controlled drugs In my country, the THC content of the medicinal cannabis cannot be higher than 0.3%, o...

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 李忠森邓艳雪刘丹
Owner 北大荒垦丰种业股份有限公司
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