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Molecular marker Indel-T-47 for close linkage with cucumber parthenocarpic main-effect QTL (quantitative trait loci)

A technology of indel-t-47 and molecular markers, applied in the field of vegetable molecular genetics and breeding, can solve the problems of difficult breeding, long time consumption, a lot of manpower and material resources, etc., and achieve close genetic distance, high accuracy and simple methods Effect

Inactive Publication Date: 2015-07-22
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] At present, the selection of cucumber parthenocarpic materials or varieties mainly relies on phenotypic identification by isolation of bunches of flowers in the field. The efficiency is not high, the breeding is difficult, and the cultivated cucumber varieties have the disadvantages of unstable parthenocarpy and being easily affected by the cultivation environment.

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  • Molecular marker Indel-T-47 for close linkage with cucumber parthenocarpic main-effect QTL (quantitative trait loci)
  • Molecular marker Indel-T-47 for close linkage with cucumber parthenocarpic main-effect QTL (quantitative trait loci)
  • Molecular marker Indel-T-47 for close linkage with cucumber parthenocarpic main-effect QTL (quantitative trait loci)

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Embodiment Construction

[0021] The F constructed on “EC1×8419s-1” in the spring and autumn of 2013 2:3 Populations were identified as parthenocarpic phenotypes. 1335 pairs of SSR primers and 173 pairs of InDel primers were selected for polymorphic primer screening between 'EC1' and '8419s-1', and then 232 pairs of polymorphic primers were selected in 16 F 2 Screening continued on a single plant, and finally 133 SSR primers and 9 InDel primers were selected at 145 F 2 PCR amplification was carried out in individual plants, the linkage map was constructed with JoinMap4.0, and the QTL related to cucumber parthenocarpy was located with Windows QTL Cartographer v2.5 software. The results showed that the major QTL Parth2.1 located on chromosome 2 could be detected in both environments, and the explainable phenotypic variation rates were 17.4% and 10.2%, respectively. from F 3:4 Screen the remaining heterozygous individual plants containing Parth2.1 in the population and construct F 4:5 group. Using th...

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Abstract

The invention discloses a molecular marker Indel-T-47 for close linkage with cucumber parthenocarpic main-effect QTL (quantitative trait loci), and belongs to the technical field of biology. The invention firstly discloses a molecular marker Indel-T-47 for close linkage with QTL Parth2.1, and further discloses a primer Indexl-T-47F / Indel-T-47R of the marker. In a segregating population containing Parth2.1, the marker is significantly related to parthenocarpy; in an all-female selfing line material containing the Parth2.1, a 155bp product can be amplified from a strong parthenocarpy strain by the marker primer Index-T-47F / Index-T-47R; the marker primer Index-T-47F / Index-T-47R is a peak marker of the Parth2.1, and is closely linked with the Parth2.1. The molecular marker for close linkage with the Parth2.1 disclosed by the invention can be applied to assistant breeding of an all-female cucumber parthenocarpy molecular marker.

Description

technical field [0001] The invention discloses a molecular marker of cucumber parthenocarpy main effect QTL, belongs to the technical field of vegetable molecular genetic breeding, and can be used for parthenocarpy ability identification and molecular marker assisted breeding of cucumber all-female germplasm. Background technique [0002] Cucumber is one of the main vegetables cultivated in a large area in my country, and has extremely high social value and economic benefits. Parthenocarpy refers to the phenomenon that the ovary develops into fruit without pollination, fertilization or other stimulation. Cucumber varieties with strong parthenocarpic ability have significant potential to increase yield, generally can increase yield by more than 20%, and parthenocarpic fruits are seedless, less pulp, thick flesh, so that the quality is improved and the commodity is good. Therefore, parthenocarpy is an important economic trait closely related to cucumber yield and quality, and...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈劲枫武喆李季钱春桃娄群峰张璐张婷李蕾张停林
Owner NANJING AGRICULTURAL UNIVERSITY
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