Application of tomato SlMPK20 gene in creating tomato genic male sterile line

Abstract

The invention discloses an application of a tomato SlMPK20 gene in creating a tomato genic male sterile line. The tomato SlMPK20 is subjected to fixed-point knockout via a CRISPR / Cas9 knockout system,so that a tomato transgenic line undergoing SlMPK20 gene specific knockout is obtained; by implementing the SlMPK20 knockout, complete abortion of pollen grains is caused, and just a few of parthenocarpic fruits are formed; however, the SlMPK20 knockout has no obvious influence to vegetative growth of plants and development of female reproductive organs; and the SlMPK20 can specially act to a development process of tomato anthers. The tomato genic male sterile line, which is created through the SlMPK20 knockout, can achieve an infertility rate of 100%; and based upon genetic analysis, it is indicated that the tomato genic male sterile line belongs to recessive genic male sterility, and the genic male sterile line can be propagated by taking corresponding wild-type tomatoes as a maintainerline. The sterile line, which is free from transgenic ingredients, can be obtained through offspring screening. The sterile line can hybridize with a great amount of tomato breeding materials (a restorer line), so that the first generation of hybrid can be obtained. With the application of the method provided by the invention, sterile traits can be rapidly led into normal tomato breeding materials; therefore, a broad application prospect is achieved in tomato cross-breeding.

Description

technical field [0001] The invention belongs to the fields of vegetable molecular breeding and plant genetic engineering, and in particular relates to the application of tomato S1MPK20 gene in establishing tomato nuclear male sterile lines. Background technique [0002] Tomato (Solanum lycopersicum L.) is a plant of the genus Tomato in the family Solanaceae. It has a large adaptability, high yield and rich nutrition, and plays an important role in the production and consumption of vegetables in my country. Utilization of heterosis is an important and effective way to increase crop yield, and the use of sterile lines can better exert heterosis. Therefore, the research on tomato male sterility is increasing day by day. The use of tomato male sterile lines for breeding can not only save manpower and material resources in breeding, but also improve breeding efficiency and ensure the purity of seeds. [0003] Nuclear male sterile breeding is one of the important ways to utilize ...

AI Technical Summary

Problems solved by technology

However, the above technical means cannot completely inhibit the transcription and translation of MAPKs in crops, and the obtained transgenic materials cannot be completely aborted; and because the MAPK genes that have been identified and have definite functions in the model plants Arabidopsis and rice are ubiquitously expressed Therefore, interfering or knocking out it not only affects reproductive development, but also affects the vegetative growth, stress resistance and disease resistance of plants, thus limiting its application in the creation of male sterile materials

However, there is no report on the use of this technology to knock out tomato genes to improve fertility-related genes.

Images