Composition for strengthening skin barrier and resisting aging as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetic preparation, in particular to a skin barrier strengthening and anti-aging composition and a preparation method and application thereof. The composition comprises an oil-in-water emulsion, and the oil-in-water emulsion comprises the following components in parts by weight: 40 to 60 parts of water, 15 to 20 parts of an oil phase component, 3 to 8 parts of an antioxidant component, 1 to 3 parts of phospholipid, 0.1 to 3 parts of a surfactant and 0.1 to 1 part of a stabilizer. The antioxidant component comprises a cudrania tricuspidata leaf extract, a chrysanthemum flavone extract and a eleocharis tuberosa peel extract. According to the present invention, the cudrania tricuspidata leaf extract and the chrysanthemum flavone extract are encapsulated in the oil phase, and the eleocharis tuberosa peel extract is encapsulated in the water phase, such that the easily-oxidized flavone and other components are stored inside so as not to easily lose activity due to oxidation.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetic preparation, in particular to a composition for strengthening skin barrier and resisting aging, and a preparation method and application thereof. Background Art

[0002] Skin care is a comprehensive approach to maintaining a healthy and comfortable skin appearance. Cosmetic formulations used for skin care typically contain both oily or oil-soluble ingredients and water or water-soluble ingredients, and are often presented as emulsions. An emulsion is a mixture of two or more liquids that are immiscible or have limited miscibility, with one liquid dispersed as small droplets in another liquid (the continuous phase). In skin care formulations, one phase is typically composed of water or a water-soluble substance and is referred to as the aqueous phase, while the second phase is typically composed of water-insoluble emollients, waxes, or similar ingredients and is referred to as the oil phase. When the oil droplets are dispersed in the continuous aqueous phase, the emulsion is referred to as an oil-in-water or O / W emulsion; when the water droplets are dispersed in the continuous oil phase, the emulsion is referred to as a water-in-oil (W / O) emulsion. Multiphase emulsions also exist, such as O / W / O or W / O / W emulsions. Emulsions can encapsulate the active ingredients in cosmetics, but are detrimental to their release. Summary of the Invention

[0003] The purpose of the present invention is to provide a composition for strengthening the skin barrier and resisting aging, as well as a preparation method and application thereof, to solve the above problems.

[0004] According to a first aspect of an embodiment of the present disclosure, a composition for strengthening the skin barrier and anti-aging is provided, wherein the composition comprises an oil-in-water emulsion, wherein the oil-in-water emulsion comprises the following components in parts by weight: 40-60 parts by weight of water, 15-20 parts by weight of an oil phase component, 3-8 parts by weight of an antioxidant component, 1-3 parts by weight of a phospholipid, 0.1-3 parts by weight of a surfactant, and 0.1-1 parts by weight of a stabilizer.

[0005] In one aspect of the embodiments of the present disclosure, preferably, the oil-in-water emulsion contains the following components in parts by weight: 45-50 parts by weight of water, 15-18 parts by weight of an oil phase component, 3-5 parts by weight of an antioxidant component, 2-3 parts by weight of a phospholipid, 0.5-1.5 parts by weight of a surfactant, and 0.3-0.8 parts by weight of a stabilizer.

[0006] In one aspect of the embodiments of the present disclosure, the antioxidant component comprises Cudrania tricuspidata leaf extract, Chrysanthemum flavonoids extract and Eleocharis churis peel extract.

[0007] In one aspect of the embodiments of the present disclosure, the Cudrania tricuspidata leaf extract is prepared by the following steps: Step 1-a: providing dried mulberry leaves, further drying them, and then crushing and sieving them to obtain mulberry leaf powder; Step 2-a: defatting the Cudrania tricuspidata powder to obtain defatted Cudrania tricuspidata powder; Step 3-a: subjecting the defatted Cudrania chufa powder to a deproteinization process to obtain deproteinized Cudrania chufa powder; Step 4-a: adding the deproteinized Cudrania leaf powder to anhydrous ethanol, heating to 65° C.-75° C. for reflux extraction, and extracting for 3-5 hours; Step 5-a: After the extraction is completed, the supernatant is collected after centrifugation; the supernatant is concentrated to obtain a concentrated solution, which is then dried to obtain a solid of the Cudrania trifoliata leaf extract.

[0008] In one aspect of the embodiments of the present disclosure, specifically, the Cudrania tricuspidata extract is prepared by the following steps: Step 1-a: providing dried mulberry leaves, further drying them, and then grinding them through a 60-mesh sieve to obtain mulberry leaf powder; Step 2-a: Wrap the Cudrania chufa powder with filter paper, put it into a Soxhlet extractor, add petroleum ether, and reflux and defatting at 50° C.-60° C. for 2-4 hours to obtain defatted Cudrania chufa powder; Step 3-a: adding the defatted Cudrania leaf powder to water, adjusting the pH to 5-6, then adding papain, and deproteinizing at 55° C.-65° C., and then inactivating the enzyme to obtain deproteinized Cudrania leaf powder; wherein the deproteinizing time is selected from 1-2 hours, and the amount of papain used is 100-150 U / mg; Step 4-a: adding the deproteinized Cudrania leaf powder to 10 times the mass of anhydrous ethanol, heating to 65° C.-70° C. for reflux extraction, and extracting for 3-4 hours; Step 5-a: After the extraction is completed, the mixture is centrifuged at 3000 r / min for 5 minutes, and the supernatant is collected; the supernatant is concentrated to obtain a concentrated solution, which is then dried to obtain a solid of the Cudrania trifoliata leaf extract.

[0009] In one aspect of the embodiments of the present disclosure, the chrysanthemum flavonoids extract is prepared by the following steps: Step 1-b: providing dried chrysanthemums, further drying them, and then grinding them through a 50-mesh sieve to obtain chrysanthemum powder; the chrysanthemums are selected from the group consisting of chrysanthemums of the Chinese genus Chrysanthemum, Helichrysanthemum dahliae, Chrysanthemum morifolium, Chrysanthemum chinense, and Chrysanthemum Step 2-b: subjecting the chrysanthemum powder to a deproteinization process to obtain deproteinized chrysanthemum powder; Step 3-b: subjecting the deproteinized chrysanthemum powder to a decolorization process to obtain decolorized chrysanthemum powder; Step 4-b: subjecting the decolorized chrysanthemum powder to ultrasonic extraction, wherein the extraction time is selected from 2-3 hours and the extraction temperature is selected from 35° C. to 40° C.; Step 5-b: After the extraction is completed, the supernatant is collected after centrifugation; the supernatant is concentrated to obtain a concentrated solution, which is then purified by AB-8 macroporous resin and then dried to obtain a solid chrysanthemum flavonoid extract.

[0010] In one aspect of the embodiments of the present disclosure, the chrysanthemum flavonoids extract is prepared by the following steps: Step 1-b: providing dried chrysanthemums, further drying, crushing and sieving to obtain chrysanthemum powder; the chrysanthemums are selected from the group consisting of chrysanthemums of the Chinese genus Chrysanthemum, Helichrysanthemum dahliae, Chrysanthemum morifolium, Chrysanthemum chinense, and Chrysanthemum truncatum; Step 2-b: adding the chrysanthemum powder to water, adjusting the pH to 5-6, then adding papain, and deproteinizing at 55° C.-65° C., and then inactivating the enzyme to obtain deproteinized chrysanthemum powder; wherein the deproteinizing time is selected from 1-2 hours, and the amount of papain used is 50-100 U / mg; Step 3-b: adding the deproteinized chrysanthemum powder to water, adding activated carbon, and standing for 5-8 hours to decolorize, thereby obtaining decolorized chrysanthemum powder; Step 4-b: subjecting the decolorized chrysanthemum powder to ultrasonic extraction, wherein the extraction time is selected from 2-3 hours and the extraction temperature is selected from 35° C. to 40° C.; Step 5-b: After the extraction is completed, the supernatant is collected after centrifugation at 3000 r / min for 5 minutes; the supernatant is concentrated to obtain a concentrated solution, which is then purified by AB-8 macroporous resin and then dried to obtain a solid chrysanthemum flavonoid extract.

[0011] In one aspect of the embodiments of the present disclosure, the water chestnut peel extract is prepared by the following steps: Step 1-c: providing water chestnut peels, crushing the peels, placing them in a 95% ethanol solution, and extracting them for 3-7 days, then filtering them using a Buchner funnel, and concentrating the obtained filtrate to an extract to obtain a water chestnut peel crude extract; Step 2-c: adding the crude water chestnut peel extract to water, extracting with petroleum ether and ethyl acetate respectively, and concentrating to obtain a solid of the water chestnut peel extract.

[0012] In one aspect of the embodiments of the present disclosure, specifically, the water chestnut peel extract is prepared by the following steps: Step 1-c: providing water chestnut peel, crushing the water chestnut peel and placing it in a 95% ethanol solution with a mass ratio of 16 times, extracting for 4-6 days, then filtering with a Buchner funnel, and concentrating the obtained filtrate to an extract to obtain a water chestnut peel crude extract; Step 2-c: The crude water chestnut peel extract is added to 5 times the mass of water to dissolve, and then an equal volume of petroleum ether is added for extraction, and the aqueous phase is retained. After adding an equal volume of ethyl acetate to the aqueous phase for extraction, the aqueous phase is retained, and the obtained aqueous phase is concentrated to obtain a solid of the water chestnut peel extract.

[0013] In one aspect of the embodiments of the present disclosure, the mass ratio of the Cudrania tricuspidata leaf extract, the Chrysanthemum flavonoids extract and the water chestnut peel crude extract is selected from 1:1:2.

[0014] In one aspect of an embodiment of the present disclosure, the oil phase component includes oil, and the oil is selected from one of hydrogenated castor oil, avocado oil, camellia seed oil, corn oil, olive oil, sesame oil, peach kernel oil, wheat germ oil, camellia oil, castor oil, linseed oil, safflower oil, cottonseed oil, perilla oil, soybean oil, peanut oil, ginkgo oil, paulownia oil, Japanese tung oil, germ oil, tricaprylin and triisopalmitin.

[0015] In one aspect of the embodiments of the present disclosure, the oil is selected from perilla oil.

[0016] In one aspect of the embodiments of the present disclosure, the phospholipid is selected from one of lecithin, soybean lecithin, cephalin, dioleoylphosphatidylcholine, hydrogenated soybean lecithin, dipalmitoylphosphatidylcholine, dioleoylphosphatidylcholine and distearoylphosphatidylglycerol.

[0017] In one aspect of the embodiments of the present disclosure, the surfactant is selected from whey protein or low-ester pectin.

[0018] In one aspect of the embodiments of the present disclosure, the stabilizer is a polyvinyl alcohol-nadic anhydride graft copolymer; the polyvinyl alcohol-nadic anhydride graft copolymer is prepared by the following steps: Step 1-d: Add polyvinyl alcohol to anhydrous ethanol, and after complete dissolution, add nadic anhydride; then add isoamyl formate and heat to 50°C-60°C for 1-3 hours; Step 2-d: After the reaction is completed, the solvent is removed and the mixture is dried to obtain the polyvinyl alcohol-nadic anhydride graft copolymer.

[0019] According to a second aspect of the embodiments of the present disclosure, there is provided a method for preparing the aforementioned skin barrier strengthening and anti-aging composition, wherein the composition comprises an oil-in-water emulsion, and the method comprises the following steps: Step 1: preparing a Cudrania tricuspidata extract and a Chrysanthemum flavonoids extract, and providing oil as an oil phase component; dissolving the Cudrania tricuspidata extract, the Chrysanthemum flavonoids extract and phospholipids in the oil phase component to obtain a first solution; Step 2: preparing a water chestnut peel extract and a polyvinyl alcohol-nadic anhydride graft copolymer, and providing a surfactant; dissolving the water chestnut peel extract, the polyvinyl alcohol-nadic anhydride graft copolymer, and the surfactant in water to obtain a second solution; Step 3: adding the first solution dropwise to the second solution at a stirring rate of 1500-2000 r / min to obtain the oil-in-water emulsion.

[0020] According to a third aspect of the embodiments of the present disclosure, a method for preparing the aforementioned composition for strengthening the skin barrier and anti-aging is provided, or the role of the composition for strengthening the skin barrier and anti-aging prepared by the aforementioned method in the field of cosmetic preparation is provided.

[0021] According to a fourth aspect of an embodiment of the present disclosure, a cosmetic composition is provided, comprising the aforementioned composition for strengthening the skin barrier and anti-aging; and the cosmetic composition further comprises at least one cosmetic excipient; the cosmetic excipient may be selected from at least one of antioxidants, moisturizers, thickeners, foaming agents, preservatives, cooling agents, buffers, pH regulators, colorants, excipients, dispersants, emulsifiers, solubilizers, surfactants, astringents, lubricants, stabilizers, and solvents, but is not limited thereto.

[0022] The technical solutions provided by the embodiments of the present disclosure may have the following beneficial effects: As can be seen from the above examples, the present disclosure prepares an ionic strength-sensitive emulsion, which encapsulates easily oxidizable anti-aging ingredients in the internal oil phase, and has good storage resistance when not in use; and the polyvinyl alcohol-nadic anhydride graft copolymer stabilizer prepared in the present disclosure has ion sensitivity to human skin. After the emulsion is applied to the skin surface, the stabilizer loses its effectiveness, the emulsion breaks, and the active ingredients are released and applied to the skin surface. DETAILED DESCRIPTION

[0023] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present application will be described in detail below with reference to Tables 1-3 and in combination with the embodiments.

[0024] In order to enable those skilled in the art to better understand the present invention, the following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with Tables 1-3 of the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

[0025] To make the purpose, technical solutions, and advantages of this application more clear, the technical solutions of this application will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of this application, rather than all the embodiments. The relevant embodiments described herein are illustrative and are used to provide a basic understanding of this application. The embodiments of this application should not be interpreted as limiting this application.

[0026] For the sake of clarity, only some numerical ranges are specifically disclosed herein. However, any lower limit may be combined with any upper limit to form an unspecified range; and any lower limit may be combined with other lower limits to form an unspecified range, and similarly, any upper limit may be combined with any other upper limit to form an unspecified range. In addition, each individually disclosed point or single value may itself serve as a lower limit or upper limit and be combined with any other point or single value, or with other lower limits or upper limits, to form an unspecified range.

[0027] As used herein, the terms "comprises," "comprising," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not preclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0028] In the description herein, unless otherwise specified, “above” and “below” include the number itself.

[0029] Unless otherwise specified, the terms used in this disclosure have the commonly understood meanings commonly understood by those skilled in the art. Unless otherwise specified, the numerical values ​​of the various parameters mentioned in this disclosure can be measured using various measurement methods commonly used in the art (for example, they can be tested according to the methods given in the examples of this disclosure).

[0030] The term "about" is used to describe and illustrate small changes. When used in conjunction with an event or situation, the term may refer to an example in which the event or situation occurs precisely and an example in which the event or situation occurs very approximately. For example, when used in conjunction with a numerical value, the term may refer to a variation range of less than or equal to ±10% of the numerical value, such as less than or equal to ±5%, less than or equal to ±4%, less than or equal to ±3%, less than or equal to ±2%, less than or equal to ±1%, less than or equal to ±0.5%, less than or equal to ±0.1%, or less than or equal to ±0.05%. In addition, amounts, ratios, and other numerical values ​​are sometimes presented in this article in a range format. It should be understood that such range formats are for convenience and brevity, and should be flexibly understood to include not only numerical values ​​explicitly designated as range limits, but also all individual numerical values ​​or subranges encompassed within the range, as if each numerical value and subrange were explicitly designated.

[0031] A list of items connected by the terms "at least one of," "at least one of," "at least one of," or other similar terms can mean any combination of the listed items. For example, if items A and B are listed, the phrase "at least one of A and B" means only A; only B; or A and B. In another example, if items A, B, and C are listed, the phrase "at least one of A, B, and C" means only A; or only B; only C; A and B (excluding C); A and C (excluding B); B and C (excluding A); or all of A, B, and C. Item A can contain a single component or multiple components. Item B can contain a single component or multiple components. Item C can contain a single component or multiple components.

[0032] In the present disclosure, the mulberry leaf extract prepared in the present disclosure contains rich oil-soluble components such as flavonoids and saponins; the chrysanthemum flavonoid extract contains rich flavonoid components; both have good anti-aging ability, and the water chestnut peel extract contains rich water-soluble components such as polysaccharides and oligosaccharides, and has good skin barrier strengthening ability; the present disclosure encapsulates the mulberry leaf extract and the chrysanthemum flavonoid extract in the oil phase, and encapsulates the water chestnut peel extract in the water phase, thereby preserving easily oxidized components such as flavonoids inside, so that they are not easily inactivated by oxidation.

[0033] The present invention discloses a polyvinyl alcohol-nadic anhydride graft copolymer prepared as a stabilizer; first, polyvinyl alcohol itself has certain hydrophilicity and film-forming properties, and the introduction of nadic anhydride gives the graft copolymer a lipophilic hydrophobic group; this amphiphilic structure enables it to be oriented at the oil-water interface, reducing the surface tension between oil and water, and helping to form a stable emulsion, which can coexist with phospholipid molecules at the oil-water interface and form a film; in the storage state, there is a certain interaction between the polyvinyl alcohol main chain and the nadic anhydride side chains in the graft copolymer, and the hydroxyl groups of polyvinyl alcohol can interact with the anhydride groups of nadic anhydride through electrostatic interaction and hydrogen bonding to form a cross-linked structure, which can enhance the thermal stability and mechanical stability of the graft copolymer; and when the emulsion is applied to the skin surface, due to the presence of sodium ions and potassium ions on the skin surface, and the rich oxygen anions (O - ), the addition of sodium / potassium ions will combine with oxygen anions, thereby destroying the electrostatic interaction and hydrogen bonding between the polyvinyl alcohol-nadic anhydride graft copolymer, causing the conformation of the polyvinyl alcohol-nadic anhydride graft copolymer chain segments to change, causing it to transition from an extended state to a contracted state, thereby affecting the film-forming properties, and ultimately leading to emulsion demulsification and the release of effective ingredients such as flavonoids from the oil phase.

[0034] The present disclosure is further described below with reference to the following examples. It should be understood that these examples are only used to illustrate the present disclosure and are not intended to limit the scope of the present disclosure.

[0035] Examples and Comparative Examples: Example 1 The first embodiment includes the following steps: 1. Preparation of Cudrania tricuspidata extract: 50 g of dried Cudrania chinensis leaves were provided, which were further dried and then pulverized through a 60-mesh sieve to obtain Cudrania chinensis leaf powder; the Cudrania chinensis leaf powder was wrapped with filter paper, placed in a Soxhlet extractor, and petroleum ether was added. The extract was refluxed at 50° C. for 3 h to obtain defatted Cudrania chinensis leaf powder; The defatted Cudrania chinensis leaf powder was added to 200 mL of water, the pH was adjusted to 5.8, and then papain was added to deproteinize the Cudrania chinensis leaf at 60° C. After deproteinization, the enzyme was inactivated to obtain the deproteinized Cudrania chinensis leaf powder; wherein the deproteinization time was 1.5 h and the amount of papain used was 120 U / mg; The deproteinized Cudrania leaf powder was added to 10 times the mass of anhydrous ethanol, heated to 70°C for reflux extraction, and the extraction time was 3 hours. After the extraction, the powder was centrifuged at 3000 r / min for 5 minutes, and the supernatant was collected. The supernatant was concentrated to obtain a concentrated solution, which was then dried to obtain a solid Cudrania leaf extract (a total of 0.87 g).

[0036] 2. Preparation of chrysanthemum flavonoids extract: 35 g of dried chrysanthemum is provided, dried, and then crushed through a 50-mesh sieve to obtain chrysanthemum powder; the chrysanthemum powder is added to water, the pH is adjusted to 5.8, and then papain is added to deproteinize at 60° C. After deproteinization, the enzyme is inactivated to obtain deproteinized chrysanthemum powder; wherein the deproteinization time is selected from 1.5 hours, and the amount of papain used is 60 U / mg; The deproteinized chrysanthemum powder was added to 150 mL of water, and then 3 g of activated carbon was added. The mixture was allowed to stand for 6 hours for decolorization to obtain decolorized chrysanthemum powder. The decolorized chrysanthemum powder was subjected to ultrasonic extraction for 2.5 hours, the ultrasonic frequency was selected from 25 kHz, and the extraction temperature was 40°C. After the extraction, the mixture was centrifuged at 3000 r / min for 5 minutes, and the supernatant was taken. The supernatant was concentrated to 1 / 7 of the original volume to obtain a concentrated solution. The concentrated solution was purified by AB-8 macroporous resin and then dried to obtain a solid (0.55 g) of chrysanthemum flavonoid extract.

[0037] 3. Preparation of water chestnut peel extract: 200 g of water chestnut peel is provided. The water chestnut peel is crushed and placed in a 95% ethanol solution with a volume of 16 times the mass thereof, and extracted for 5 days. The water chestnut peel is then filtered using a Buchner funnel, and the obtained filtrate is concentrated to an extract to obtain a water chestnut peel crude extract. The water chestnut peel crude extract is added to 5 times the mass of water to dissolve, and then an equal volume of petroleum ether is added for extraction, and the aqueous phase is retained. After extraction, an equal volume of ethyl acetate is added to the aqueous phase, and the aqueous phase is retained. The obtained aqueous phase is concentrated to obtain a solid (1.74 g) of the water chestnut peel extract.

[0038] The above steps can be repeated multiple times to obtain a sufficient amount of product.

[0039] 4. Preparation of polyvinyl alcohol-nadic anhydride graft copolymer: 5 g of polyvinyl alcohol (pva-2055) was added to 30 mL of anhydrous ethanol. After it was completely dissolved, 0.65 g of nadic anhydride was added. Then, 0.1 g of isoamyl formate was added and the mixture was heated to 55° C. and reacted for 1.5 hours. After the reaction was completed, the solvent was removed by rotary evaporation, and the solid of the polyvinyl alcohol-nadic anhydride graft copolymer was obtained after drying.

[0040] 5. Prepare emulsion: 1 part by weight of Cudrania tricuspidata extract and 1 part by weight of Chrysanthemum flavonoids extract were weighed and dissolved with 2 parts by weight of lecithin in 16 parts by weight of Perilla oil; 1 part by weight of low-ester pectin and 0.5 parts by weight of polyvinyl alcohol-nadic anhydride graft copolymer were dissolved in 45 parts by weight of water; the oil phase solution was added dropwise to the aqueous phase solution at a stirring rate of 1750 r / min to obtain the oil-in-water emulsion of Example 1.

[0041] Comparative Example 1 Comparative Example 1 comprises the following steps: 1. Preparation of polyvinyl alcohol-nadic anhydride graft copolymer: 5 g of polyvinyl alcohol (pva-2055) was added to 30 mL of anhydrous ethanol. After it was completely dissolved, 0.65 g of nadic anhydride was added. Then, 0.1 g of isoamyl formate was added and the mixture was heated to 55° C. and reacted for 1.5 hours. After the reaction was completed, the solvent was removed by rotary evaporation, and the solid of the polyvinyl alcohol-nadic anhydride graft copolymer was obtained after drying.

[0042] 2. Prepare emulsion: 2 parts by weight of lecithin were weighed and dissolved in 16 parts by weight of perilla oil; 1 part by weight of low-ester pectin and 0.5 parts by weight of polyvinyl alcohol-nadic anhydride graft copolymer were dissolved in 45 parts by weight of water; and the oil phase solution was added dropwise to the aqueous phase solution at a stirring rate of 1750 r / min to obtain the oil-in-water emulsion of Comparative Example 1.

[0043] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain Cudrania tricuspidata extract, Chrysanthemum flavonoids extract and Water Chestnut peel extract.

[0044] DPPH free radical scavenging activity assay: For Example 1 and Comparative Example 1, after demulsification, 500 μL of each 30 mg / mL sample solution was placed in a 96-well plate. 100 μL of a 0.04 mg / mL DPPH ethanol solution was added to each well. The reaction was incubated at room temperature in the dark for 30 minutes. In the control group, the DPPH ethanol solution was replaced with 100 μL of anhydrous ethanol, in the blank group, the sample solution was replaced with 100 μL of deionized water, and in the positive control, the sample solution was replaced with 100 μL of ascorbic acid. The remaining preparation methods were the same as for the test solutions. The absorbance was measured at 517 nm, and the experiment was repeated three times. Clearance (%) = [1 - [A sample - A control group] / A blank group] × 100%.

[0045] Determination of hydroxyl free radical scavenging ability: 0.2 mL of FeSO4-EDTA mixed solution (10.0 mmol / L) was placed in a test tube, 0.5 mL of 2-deoxyribose solution (10.0 mmol / L) and 1.0 mL of 30 mg / mL solution of each sample of Example 1 and Comparative Example 1 were added, the volume was made up to 1.8 mL with phosphate buffer (pH = 7.4, 0.1 mol / L), 0.2 mL of H2O2 (10.0 mol / L) was added, the mixture was mixed and placed in a 37°C constant temperature water bath to react for 1 hour; then 1.0 mL of 2.8% (w / w) trichloroacetic acid (TCA) solution was added, the mixture was centrifuged at 4000 r / min for 20 minutes, 2.0 mL of the supernatant was placed in another test tube, 1.0 mL of 1.0% (w / w) thiobarbituric acid (TBA) solution was added, the mixture was mixed and placed in a boiling water bath to react for 15 minutes, the mixture was cooled and diluted 5 times, and the absorbance was measured at a wavelength of 532 nm. Scavenging rate SA (%) = [(Ac-As) / (Ac-A0)] × 100%; where: Ac is the absorbance of the mixed solution; As is the absorbance after adding the samples of Example 1 and Comparative Example 1; A0 is the absorbance of the reagent blank.

[0046] The test results are shown in Table 1 below: Table 1 Example 2 The steps of Example 2 are the same as those of Example 1, except that when preparing the copolymer stabilizer, nadic anhydride is replaced with an equimolar amount of maleic anhydride to obtain a polyvinyl alcohol-maleic anhydride graft copolymer.

[0047] Example 3 The steps of Example 3 are the same as those of Example 1, except that when preparing the copolymer stabilizer, nadic anhydride is replaced with an equal molar amount of succinic anhydride to obtain a polyvinyl alcohol-succinic anhydride graft copolymer.

[0048] Example 4 The steps of Example 4 are the same as those of Example 1, except that when preparing the copolymer stabilizer, nadic anhydride is replaced with an equal molar amount of phthalic anhydride to obtain a polyvinyl alcohol-phthalic anhydride graft copolymer.

[0049] Example 5 The steps of Example 5 are the same as those of Example 1, except that when preparing the copolymer stabilizer, nadic anhydride is replaced with an equimolar amount of pyromellitic dianhydride to obtain a polyvinyl alcohol-pyromellitic dianhydride graft copolymer.

[0050] Storage performance test: The products of Examples 1 to 5 were stored at room temperature without light protection or sealing treatment. The storage stability of the samples of Examples 1 to 5 was observed, and whether they broke after 1 day, 3 days and 7 days respectively. The results are shown in Table 2 below.

[0051] Table 2 Among them, the “-” in Table 1 means that the next step is not recorded after the emulsion is broken.

[0052] Skin surface testing: Forty subjects, 20 males and 20 females aged 20-35, were selected to test the products of Examples 1 to 5. Approximately 5-8 drops of each of Examples 1 to 5 were applied to the subjects' facial skin. The emulsion droplets were collected with a dropper after 1 minute, 10 minutes, and 30 minutes, respectively, and observed under a microscope to determine if the emulsion had broken. The results are shown in Table 3 below.

[0053] Table 3 Those skilled in the art will readily appreciate other embodiments of the present disclosure after considering the specification and practicing the disclosure disclosed herein. This disclosure is intended to cover any variations, uses, or adaptations of the present disclosure that follow the general principles of the present disclosure and include common knowledge or customary techniques in the art not disclosed herein.

Claims

1. A composition for strengthening skin barrier and anti-aging, characterized in that: The composition comprises an oil-in-water emulsion, and the oil-in-water emulsion comprises the following components in parts by weight: 40-60 parts by weight of water, 15-20 parts by weight of oil phase components, 3-8 parts by weight of antioxidant components, 1-3 parts by weight of phospholipids, 0.1-3 parts by weight of surfactants and 0.1-1 parts by weight of stabilizers.

2. The composition for strengthening the skin barrier and resisting aging according to claim 1, characterized in that: The antioxidant component comprises Cudrania tricuspidata leaf extract, Chrysanthemum flavonoids extract and Water Chestnut peel extract.

3. The composition for strengthening the skin barrier and resisting aging according to claim 2, characterized in that: The Cudrania tricuspidata leaf extract is prepared by the following steps: Step 1-a: providing dried mulberry leaves, further drying them, and then crushing and sieving them to obtain mulberry leaf powder; Step 2-a: defatting the Cudrania tricuspidata powder to obtain defatted Cudrania tricuspidata powder; Step 3-a: subjecting the defatted Cudrania chufa powder to a deproteinization process to obtain deproteinized Cudrania chufa powder; Step 4-a: adding the deproteinized Cudrania leaf powder to anhydrous ethanol, heating to 65° C.-75° C. for reflux extraction, and extracting for 3-5 hours; Step 5-a: After the extraction is completed, the supernatant is collected after centrifugation; the supernatant is concentrated to obtain a concentrated solution, which is then dried to obtain a solid of the Cudrania trifoliata leaf extract.

4. The composition for strengthening the skin barrier and resisting aging according to claim 2, characterized in that: The chrysanthemum flavonoids extract is prepared by the following steps: Step 1-b: providing dried chrysanthemums, further drying, crushing and sieving to obtain chrysanthemum powder; the chrysanthemums are selected from the group consisting of chrysanthemums of the Chinese genus Chrysanthemum, Helichrysanthemum dahliae, Chrysanthemum morifolium, Chrysanthemum chinense, and Chrysanthemum truncatum; Step 2-b: subjecting the chrysanthemum powder to a deproteinization process to obtain deproteinized chrysanthemum powder; Step 3-b: subjecting the deproteinized chrysanthemum powder to a decolorization process to obtain decolorized chrysanthemum powder; Step 4-b: subjecting the decolorized chrysanthemum powder to ultrasonic extraction, wherein the extraction time is selected from 2-3 hours and the extraction temperature is selected from 35° C. to 40° C.; Step 5-b: After the extraction is completed, the supernatant is collected after centrifugation; the supernatant is concentrated to obtain a concentrated solution, which is then purified by AB-8 macroporous resin and then dried to obtain a solid chrysanthemum flavonoid extract.

5. The composition for strengthening skin barrier and resisting aging according to claim 2, characterized in that: The water chestnut peel extract is prepared by the following steps: Step 1-c: providing water chestnut peels, crushing the peels, placing them in a 95% ethanol solution, and extracting them for 3-7 days, then filtering them using a Buchner funnel, and concentrating the obtained filtrate to an extract to obtain a water chestnut peel crude extract; Step 2-c: adding the crude water chestnut peel extract to water, extracting with petroleum ether and ethyl acetate respectively, and concentrating to obtain a solid of the water chestnut peel extract.

6. The composition for strengthening skin barrier and resisting aging according to claim 1, characterized in that: The oil phase component includes oil, which is selected from one of hydrogenated castor oil, avocado oil, camellia seed oil, corn oil, olive oil, sesame oil, peach kernel oil, wheat germ oil, camellia oil, castor oil, linseed oil, safflower oil, cottonseed oil, perilla oil, soybean oil, peanut oil, torreya oil, paulownia oil, Japanese tung oil, germ oil, tricaprylin and triisopalmitin; The phospholipid is selected from one of lecithin, soybean lecithin, cephalin, dioleoylphosphatidylcholine, hydrogenated soybean lecithin, dipalmitoylphosphatidylcholine, dioleoylphosphatidylcholine and distearoylphosphatidylglycerol; The surfactant is selected from whey protein or low-ester pectin.

7. The composition for strengthening skin barrier and resisting aging according to claim 1, characterized in that: The stabilizer is a polyvinyl alcohol-nadic anhydride graft copolymer; the polyvinyl alcohol-nadic anhydride graft copolymer is prepared by the following steps: Step 1-d: Add polyvinyl alcohol to anhydrous ethanol, and after complete dissolution, add nadic anhydride; then add isoamyl formate and heat to 50°C-60°C for 1-3 hours; Step 2-d: After the reaction is completed, the solvent is removed and the mixture is dried to obtain the polyvinyl alcohol-nadic anhydride graft copolymer.

8. A method for preparing the skin barrier strengthening and anti-aging composition according to any one of claims 1 to 7, characterized in that: The composition comprises an oil-in-water emulsion, and the method comprises the following steps: Step 1: preparing a Cudrania tricuspidata extract and a Chrysanthemum flavonoids extract, and providing oil as an oil phase component; dissolving the Cudrania tricuspidata extract, the Chrysanthemum flavonoids extract and phospholipids in the oil phase component to obtain a first solution; Step 2: preparing a water chestnut peel extract and a polyvinyl alcohol-nadic anhydride graft copolymer, and providing a surfactant; dissolving the water chestnut peel extract, the polyvinyl alcohol-nadic anhydride graft copolymer, and the surfactant in water to obtain a second solution; Step 3: adding the first solution dropwise to the second solution at a stirring rate of 1500-2000 r / min to obtain the oil-in-water emulsion.

9. Use of the method for strengthening the skin barrier and anti-aging composition according to any one of claims 1 to 7, or the skin barrier strengthening and anti-aging composition prepared by the method according to claim 8 in the field of cosmetic preparation.

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