The invention relates to naphthopyrone compounds, and a preparation method and application thereof, belonging to the field of pharmaceutical chemistry. The six naphthopyrone compounds have the advantages of simple preparation process, simple raw materials, convenient after-treatment, high yield and high purity (up to 98% or above), and the structure can be verified through nuclear magnetism and mass spectroscopy. After the six naphthopyrone compounds are used for preparing antineoplastic drugs and the obtained antineoplastic drugs are subjected to anticancer activity research on A549 cells (human non-small cell lung cancer cells), the research result on the apoptosis by the real-time cell analysis technique (RTCA) proves that the compounds L2 and L3 have strong killing actions on the A549 cells, the compound L1 has weaker killing actions, and the compounds L4, L5 and L5 have the weakest killing actions. Therefore, the invention has substantial meanings for research into screening of the antineoplastic drugs of the compounds.