Use of MAGE-A9
A MAGE-A9, 1. MAGE-A9 technology, applied in the field of cancer drugs, can solve the problem that the impact of lung cancer invasion and migration has not been studied, and achieve the effect of reducing cell migration and invasion ability
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Embodiment 1
[0021] 180 lung adenocarcinoma tissue slices and 94 paracancerous tissue slices were taken. All slices were taken from patients who were hospitalized for surgical treatment from January 2004 to December 2009 in the Department of Cardiothoracic Surgery of the Affiliated Hospital of Nantong University. All patients had not undergone radiotherapy and chemotherapy before operation, and clinical data (including age, sex, stage, tumor size, differentiation, lymph node metastasis and distant metastasis) were recorded in writing. The above-mentioned 180 cases have complete medical history and follow-up records. The cut-off date of follow-up is May 2014, and the follow-up rate is 100%.
[0022] Immunohistochemical method specimens: 180 cases of lung adenocarcinoma tissue above, all specimens were confirmed by pathology, tumor specimens were taken from the central part of tumor resection during operation, and 94 cases of corresponding paracancerous tissues, all specimens were from the De...
Embodiment 2
[0045] 1) siRNA design
[0046] Three different siRNA sequences against human MAGE-A9 and negative control siRNA (negative control, NC) were designed and completed by Shanghai Invitrogen Company, the sequences are as follows:
[0047] MAGE-A9#1: sense strand: 5'-GGUGGCUGAGUUGGUUCAUTT-3';
[0048] Antisense strand: 5'-AUGAACCAACUCAGCCACCTT-3';
[0049] MAGE-A9#2: sense strand: 5'-GCAAAGCCUCCGAGUUCAUTT-3';
[0050] Antisense strand: 5'-AUGAACUCGGAGGCUUUGCTT-3';
[0051] MAGE-A9#3: sense strand: 5'-CCAGCUAUGAGAAGGUCAUTT-3';
[0052] Antisense strand: 5'-AUGACCUUCUCAUAGCUGGTT-3'.
[0054] (1) Take out the cryopreservation tubes of A549, SPC-A-1, NCI-H1975, and NCI-H1650 cells from the -80°C ultra-low temperature freezer, and place them in a 37°C water bath for rapid shaking to completely thaw the cells within 1 min. (2) Take out the cryopreservation tube, disinfect it with alcohol, and put it into the ultra-clean bench. (3) Pipette the cell suspen...
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