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Use of MAGE-A9

A MAGE-A9, 1. MAGE-A9 technology, applied in the field of cancer drugs, can solve the problem that the impact of lung cancer invasion and migration has not been studied, and achieve the effect of reducing cell migration and invasion ability

Inactive Publication Date: 2015-12-23
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing literature, the expression of MAGE-A9 in NSCLC adenocarcinoma and its relationship with clinicopathological parameters, and the effect of MAGE-A9 on the invasion and migration of lung cancer have not been studied yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 180 lung adenocarcinoma tissue slices and 94 paracancerous tissue slices were taken. All slices were taken from patients who were hospitalized for surgical treatment from January 2004 to December 2009 in the Department of Cardiothoracic Surgery of the Affiliated Hospital of Nantong University. All patients had not undergone radiotherapy and chemotherapy before operation, and clinical data (including age, sex, stage, tumor size, differentiation, lymph node metastasis and distant metastasis) were recorded in writing. The above-mentioned 180 cases have complete medical history and follow-up records. The cut-off date of follow-up is May 2014, and the follow-up rate is 100%.

[0022] Immunohistochemical method specimens: 180 cases of lung adenocarcinoma tissue above, all specimens were confirmed by pathology, tumor specimens were taken from the central part of tumor resection during operation, and 94 cases of corresponding paracancerous tissues, all specimens were from the De...

Embodiment 2

[0045] 1) siRNA design

[0046] Three different siRNA sequences against human MAGE-A9 and negative control siRNA (negative control, NC) were designed and completed by Shanghai Invitrogen Company, the sequences are as follows:

[0047] MAGE-A9#1: sense strand: 5'-GGUGGCUGAGUUGGUUCAUTT-3';

[0048] Antisense strand: 5'-AUGAACCAACUCAGCCACCTT-3';

[0049] MAGE-A9#2: sense strand: 5'-GCAAAGCCUCCGAGUUCAUTT-3';

[0050] Antisense strand: 5'-AUGAACUCGGAGGCUUUGCTT-3';

[0051] MAGE-A9#3: sense strand: 5'-CCAGCUAUGAGAAGGUCAUTT-3';

[0052] Antisense strand: 5'-AUGACCUUCUCAUAGCUGGTT-3'.

[0053] 2) Cell recovery:

[0054] (1) Take out the cryopreservation tubes of A549, SPC-A-1, NCI-H1975, and NCI-H1650 cells from the -80°C ultra-low temperature freezer, and place them in a 37°C water bath for rapid shaking to completely thaw the cells within 1 min. (2) Take out the cryopreservation tube, disinfect it with alcohol, and put it into the ultra-clean bench. (3) Pipette the cell suspen...

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Abstract

The invention discloses an application of MAGE-A9 in the preparation of lung cancer treatment drugs. The MAGE-A9 is a target for non-small cell lung cancer gene therapy, and the drugs are designed against the target. Influences of down-regulation of MAGE-A9 gene expression on NSCLC (non-small cell lung cancer) cell invasion, migration and other biological behaviors are researched in test of in vitro environment, and a result of the researches shows that a specific siRNA sequence can effectively inhibit expression of the MAGE-A9 protein in the NSCLC cell strains SPC-A-1 and NCI-H1975. After the MAGE-A9 expression is inhibited through RNA interference, propagation of the SPC-A-1 and NCI-H1975 cells is slow, apoptosis increases, and the cell migration and invasion ability is reduced. The MAGE-A9 is the target for non-small cell lung cancer gene therapy, and can be widely applied in the preparation of the lung cancer treatment drugs.

Description

technical field [0001] The invention belongs to the technical field of cancer drugs, and specifically relates to a use of MAGE-A9. Background technique [0002] Lung cancer is the most common type of cancer and the leading cause of cancer-related deaths worldwide. Lung cancer can be divided into non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). The prognosis of patients with NSCLC depends largely on the stage at diagnosis. Despite early screening, most lung cancers are detected at an advanced stage, and approximately 85% of these lung cancers are NSCLC. Although a variety of treatment methods are used, including surgery, radiotherapy, chemotherapy, and biological therapy, the prognosis of these patients is still poor, with a 5-year survival rate of about 10% and a median survival time of 16 to 18 months. With the development of gene transfer technology, gene targeted therapy has become a new method of NSCLC treatment. Therefore, exploring new biomolec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57423
Inventor 茅国新黄剑飞翟晓璐许立芹张思雅
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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