47 results about "Surgical specimen" patented technology

The present invention provides a surgical specimen retrieval device that allows one-handed use by incorporating a geared spool to amplify take up of a flexible line to close the mouth of the specimen bag after the surgical specimen is deposited in the bag. The retrieval device includes a spool with an attached gear that is rotated by movement of a deployment rod with serrated teeth. A flexible line has a proximal end attached to the spool and the distal end attached to the mouth of the bag. When the deployment reel is moved from an extended position to an inserted position, the spool rotates and pays out flexible line to allow the mouth of the deployed bag to open. Once a specimen is taken and deposited in the bag, the deployment rod is moved from the inserted position toward the extended position. This rod movement rotates the spool to take up the line and close the mouth of the bag. When the rod is moved to a fully retracted position, the teeth on the rod disengage from the gear, which allows the spool to rotate freely. The device is then removed from the trocar and the line is used to pull the bagged specimen from the body cavity.

The invention discloses a non-small cell lung cancer cell line, and an establishment method and application thereof. The human non-small cell lung cancer cell line is preserved in China Center for Type Culture Collection with an accession number of CCTCC NO: C2012128. The establishment method comprises the following steps: step 1, acquiring a fresh clinical human non-small cell lung cancer surgical specimen, cutting the specimen into small blocks with a size of 20 to 50 mg and inoculation mammals with the block through subcutaneous puncture; and step 2, in 70 to 90 days after the puncture-inoculation, killing a tumor-bearing animal, taking tumor tissue out of the tumor-bearing animal and carrying out primary culture and subculture of cancer cells. According to the invention, the human non-small cell lung cancer cell line has the advantages of stable properties, capability of realizing stable multiple passage, a high tumor formation rate, a short latent period and good homogeneity; meanwhile, the human non-small cell lung cancer cell line has acquired drug resistance to gefitinib, can be used to analyze correlation between in-vitro and in-vivo drug sensibilities and drug resistance and is the ideal cell line for basic research on and preclinical application of human non-small cell lung cancer.

The present invention relates to diagnostic methods that are relevant to various solid tumor cancers that are not amenable to traditional BIH3 profiling diagnostic methods. In some embodiments, the methods described herein are useful in the evaluation of a patient, for example, for evaluating diagnosis, prognosis, and response to treatment. In various aspects, the present disclosure includes evaluating a solid tumor or cancer. In various embodiments, the evaluation may be selected from diagnosis, prognosis, and response to treatment. In various embodiments, the present disclosure directs the treatment of a cancer patient, including, for example, what type of treatment should be administered or withheld. In some embodiments, the present disclosure includes the measurement of a tumor cell, sample, and/or specimen, including biopsy or surgical specimen samples.

The invention relates to a disposable specimen taking-out device under a cystoscope, a ureteroscope and a nephroscope. The specimen taking-out device is composed of an inner sleeve, a push-pull rod, a handle, a metal ring and a filtering object taking-out bag. The length of the inner sleeve is greater than that of a cystscope sheath, the length of the push-pull rod is greater than that of the inner sleeve, one end of the push-pull rod is provided with the handle, the other end is provided with the metal ring, the metal ring is sleeved by the filtering object taking-out bag, the filtering object taking-out bag only can filter liquids and cannot filter tumor cells and tissues, the metal ring is elastic, the push-pull rod, the metal ring and the filtering object taking-out bag can be placed in the inner sleeve, and when the metal ring is pushed out from the inner sleeve, the metal ring is expanded so as to open the bag mouth of the filtering object taking-out bag. The disposable specimen taking-out device provided by the invention has the following advantages: compared to the prior art, the disposable specimen taking-out device is reasonable in structural design, can effectively take operation specimens completely out of bladders, ureters or renal pelvis and renal calyces, does not cause avulsion when the specimens are taken out, is more convenient and rapid to take the specimens out, takes out the specimens completely without residuals, and improves the efficiency and the treatment effect of an operation.

Differentially methylated oral squamous cell carcinoma (OSCC) biomarkers, identified in-vitro and validated in well-characterized surgical specimens, have shown poor clinical correlation in cohorts with different risk profiles. To overcome this lack of relevance we used the HumanMethylation27 BeadChip, publicly available methylation and expression array data, and Quantitative Methylation Specific PCR to uncover differential methylation in OSCC clinical samples with heterogeneous risk profiles. A two stage-design consisting of Discovery and Prevalence screens was used to identify differential promoter methylation and deregulated pathways in patients diagnosed with OSCC and head and neck squamous cell carcinoma. This Phase I Biomarker Development Trial identified a panel of differentially methylated genes in normal and OSCC clinical samples from patients with heterogeneous risk profiles. This panel may be useful for early detection and cancer prevention studies.

The invention relates to rapid surgical specimen recording paper and a recording method thereof. The recording paper comprises a piece of paper, wherein the paper can be coordinate paper; a material object dissection specimen picture layer of human organs is printed in a left upper corner area of the paper; a specimen outline line picture layer which corresponds to the material object dissection specimen at the left upper corner is printed in a middle area of the paper; a basic patient information picture layer is printed on the lower part of the paper; and the basic patient information comprises names, gender, age and the like. After a surgery is finished, the cut specimen is arranged at the corresponding position of the specimen outline picture layer, an image is acquired through an image acquisition device and is stored, and related information of the patients and the specimens can be rapidly and accurately recorded, so that the recording paper can be used for filing, research, exchange and teaching and has a high application value.

The invention discloses a surgical tumor specimen collecting device. The device comprises straw, a circular suction cup is arranged at the end of the straw, adjusting rods are arranged on the upper and lower sides of the suction cup and can adjust the diameter of the suction cup, the inside of the suction cup is provided with a liquid suction pipe and a specimen grabbing pipe, the liquid suction pipe and the specimen grabbing pipe both penetrate a piston rod in the straw, the head of the liquid suction pipe is provided with a liquid suction needle located in the suction cup, the head of the specimen grasping pipe is provided with a specimen grabbing hook located in the suction cup, the inside of the specimen grabbing pipe is further provided with a cover plate capable of extending the specimen grabbing pipe to cover the specimen grabbing hook, the other side of the cover plate is provided with an operation rod extending outside the specimen grabbing pipe, the bottom of the straw is provided with a first recovery portion and a second recovery portion which are communicated with the straw, and the first recovery portion is located at the front end of the second recovery portion. Thedevice achieves rapidly tumor tissue collecting and vacuum collecting to prevent the contamination problem in the collection process and transportation process, and the collected amount is relativelylarger, which is convenient for later researches.

The invention provides a kit for detecting the prognosis of patients with esophageal squamous cell carcinoma in the Xinjiang region. The kit comprises a phospholipase epsilon 1 protein, an IKB alpha protein and a mutant P53 protein. The kit also comprises a citrate buffer solution with the content of 0.01M, an ethylenediaminetetraacetic acid repair solution with the content of 10mM, a H2O2 solution with the mass concentration of 3%, a phosphate buffer solution with the content of 0.01M, a BSA blocking solution with the mass concentration of 5%, an immunohistochemical Envision kit and a DAB developing reagent. The kit can forecast the prognosis of patients through detecting the expression case of the paraffin-embedded surgical specimen of the patients with esophageal squamous cell carcinomathrough PLCE1, IKB alpha and P53 proteins. Compared with the existing kit realizing single index detection, the kit has higher prediction effects and has the prediction ability similar to the TNM staged prediction ability. The two-step immunohistochemical method using the kit is a mature and reliable method that can be widely used in primary hospitals.

The present invention provides a method of peptide histochemical diagnosis to detect the peptide binding protein in the cancer tissue. This peptide binding specifically to tumor cells is linked to the dextran coated iron oxide nanoparticle. The peptide linked dextran coated iron oxide nanoparticle can be used to bind to the formalin-fixed and paraffin-embedded tumor surgical specimens, and the method of present invention can be used to evaluate the efficacy of peptide-targeted chemotherapy for treatment of cancer patients.

The invention discloses a sample taking-out device for assisting a colonoscopic surgery in the technical field of medical care equipment. The sample taking-out device comprises an object taking pipe,wherein a sleeve is sleeved and connected onto the outer wall of the object taking pipe; three support plates are uniformly arranged at the bottom of the outer wall of the object taking pipe; a position limiting ring is arranged on the top of the outer wall of the object taking pipe; three linkage plates are uniformly arranged in the middle part of the outer wall of the sleeve; the other ends of the linkage plates and the other ends of the support plates are provided with annular expansion plates; a rubber ring is sleeved and connected onto the top of the outer wall of the sleeve; three connecting rods are uniformly installed on the top of the rubber ring; connecting pipes are arranged on the tops of the three connecting rods; an anti-slip sleeve is sleeved and connected onto the outer wall of the connecting pipe; through the combination of devices of the expansion plate, the sleeve, the linkage plate and the like arranged on the device, the expansion on the anus is convenient; the crissum muscle contraction is relieved; the taking of giant adenoma is convenient; the influence on pathological examination is reduced.

The invention belongs to the technical field of medical instruments, and relates to a surgical specimen fixing device, in particular to a specimen storing and transferring system for an operating roomand a specimen submitting method. The system comprises a plurality of operating rooms, a specimen area and a pathological room, wherein the specimen area and the pathological room are arranged on thesame wall; a hollow area is formed in a wall shared by the specimen area and the pathological room, and a specimen cabinet is embedded in the hollow area; a specimen storage area, a pathological biopsy area and a storage area are arranged in the specimen cabinet, the specimen storage area and the pathological biopsy area are used for communicating the specimen area with the pathological room, andthe specimen storage area and the pathological biopsy area are provided with taking and delivering openings in the specimen area and the pathological room; a code making device is installed in the specimen area, a code reading device is installed in the pathological room, information codes of in-vitro specimens in the operating room are made through the code making device in the specimen area, and the information codes are used for reading information through the code reading device in the pathological room.

The invention discloses a hepatobiliary surgical specimen disinfection and manufacturing device which comprises a cylinder body, a card slot, a mounting box, a first electric pushing rod, a gate, a cotton block, an alcohol box, a disinfection hole, a ring, a connecting rod, a hook, a sleeve, a piston rod, a specimen smearing hole, a suction disc, a cover slip, a glass slide, a second electric pushing rod, a transverse plate and a through hole. The hepatobiliary surgical specimen disinfection and manufacturing device has a simple structure, and is convenient to operate, high in functionality and novel in design. By arranging the piston and the electric pushing rods, a specimen can be manufactured better, the workload of an experimenter is reduced, the working burden is reduced, and meanwhile, the cover slip and the glass slide can be fitted more neatly and a lot of time is saved; and since cover slip and the glass slide can be directly disinfected in the cylinder, bacterial infection caused in the moving process of the cover glass and the glass slide is avoided, the safety coefficient is high, the specimen detection accuracy is improved, the working efficiency of manufacturing the specimen is improved and the practicability is improved.

The invention provides a device for taking a surgical specimen through a natural orifice. A control device is hinged to a grabbing device through a hollow connecting rod, a through sliding groove is formed in the middle of the connecting rod, a control handle moving axially is arranged in the sliding groove, and the control handle slides in the sliding groove to drive a hinge base to move forwards and backwards to control the opening and closing of the grabbing device; and the grabbing device is of a claw-shaped structure formed by a plurality of dispersedly arranged grippers, each gripper consists of a first knuckle and a second knuckle which are hinged end to end, the connecting rod is hinged to the middle of the first knuckle, the control handle is hinged to the head end of the first knuckle, the length of the control handle is greater than that of the connecting rod, the portion, between the hinged portions of the control handle and the connecting rod, of the control handle is sleeved with a spring, the second knuckle of the grabbing device is sleeved with a protective sleeve, the protective sleeve is made into two layers in the corresponding position sleeving the second knuckle, the inner layer is completely closed, the outer layer is provided with a fixing piece for the second knuckle and the protective sleeve, the end of the protective sleeve is longer than the second knuckle, and a pull rope capable of tightening a protective sleeve opening is embedded in the end of the protective sleeve.

The invention relates to a method applicable to the estimation of the prognosis of ovarian cancer patients after operation. Since ovarian cancer patients has high mortality and existing prognosis evaluation systems are tedious and not accurate enough, the invention is characterized in that the expression intensity of various TRPC protein subtypes are detected combinedly via a immunohistochemical method on surgical specimens of ovarian cancer patients, and the values are processed by a friendly man-machine conversation software so as to obtain a relatively accurate evaluation of ovarian cancer prognosis.

The invention relates to an accurate dosing device for surgical specimen stationary liquid and a using method thereof. The device comprises a box body and a conveying device arranged in the box body,and the conveying device comprises a base, a supporting plate and a conveying belt. The two ends of the supporting plate are each provided with a pair of supporting rods. Rotatable rollers are arranged between the supporting rods; the conveying belt is arranged on the rollers; a clamping mechanism, a measuring container and a specimen placing disc are arranged on the conveying belt; a storage boxand an operation hole are arranged at the top end of the box body; a liquid adding pipe is arranged at the bottom of the storage box; the liquid adding pipe extends into the box body; a control valveis arranged on the liquid adding pipe; movable doors are arranged on two sides of the box body; a specimen bag is clamped on the clamping mechanism; and scales are arranged on the outer walls of the box body and the measuring container. According to the invention, accurate addition of the specimen stationary liquid can be realized, cell autolysis or DNA degradation caused by insufficient additionof the stationary liquid is prevented, and the pathological diagnosis accuracy is effectively improved.

The invention relates to a surgical specimen taking-out kit. The surgical specimen taking-out kit comprises a storage bag, an operating rod and an operating handle, wherein the storage bag comprises abag body and an opening device, the opening device is of an annular structure, the two ends of the opening device are provided with connectors respectively, and a bag opening of the bag body sleevesthe opening device. The operating rod comprises a sleeve and a transmission rod, the transmission rod is arranged in the sleeve, the sleeve is detachably fixed to the operating handle, and one end ofthe transmission rod is used for detachably connecting the corresponding connector; the operating handle is of a gun-shaped structure and comprises a shell, a handle body, a trigger and a tightening device, wherein the other end of the transmission rod extends into the shell and is connected with the tightening device, and the tightening device is used for tightening or releasing the bag body under the control of the trigger. The provided surgical specimen taking-out kit is compact in structure, easy to operate and convenient to use; not only can isolation of tumors be achieved, but also the surgical specimen taking-out kit can be repeatedly used by replacing the storage bag, so that the cost is effectively reduced.

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. Novel treatment strategies derived from increased knowledge of molecular oncology are constantly being developed to cure this disease. Here, we used phage display to identify novel peptides, including (SP94), which binds specifically to HCC cells. In vitro, the phage clone PC94 binds to HCC cell lines. In vivo, PC94 homed specifically to tumor tissues but not to normal visceral organs in SCID mice bearing human HCC xenografts. The homing ability could be competitively inhibited by synthetic peptide, SP94. PC94 localized to tumor tissues but could not be detected in SP94-competed tumor tissues or in normal organs. In addition, PC94 recognized the tumor tissue but not non-tumor tissue in surgical specimens from HCC patients, with a positive rate of 61.3% (19/31). With the conjugation of SP94 and liposomal doxorubicin, a targeted drug delivery system enhanced the therapeutic efficacy against HCC xenografts through enhanced tumor apoptosis and decreased tumor angiogenesis. Our results indicate that SP94 can improve the systemic treatment of patients with advanced HCC.

The invention discloses a laparoscope specimen fetching channel. The laparoscope specimen fetching channel comprises a puncture outfit and a conical core. The puncture outfit is of a tubular structureand is made of flexible materials, and the conical core can pass through the interior of the tube. The puncture outfit is hollow in the middle. Two groups of supporting rod are symmetrically arrangedin the puncture outfit. The upper end of each group of supporting rod is in transmission connection with a transmission rod. Curve-shaped metal rods are connected between the two groups of supportingrods in a crossed manner. The lower end of the metal rods are fixedly connected with the supporting rods through fixing screws, and the upper ends are vertically up and down movably connected with the supporting rods through movable screws. After a laparoscopic surgery specimen is amputated, the puncture outfit is placed, and the transmission rods drive the supporting rods to rotate, so movable screws on the supporting rods slide towards the lower end of the supporting rods, so that the inclination angle of metal rods is increased and puncture outfit wall flexible materials are expanded, making an abdominal wall puncture channel expanded, thereby being beneficial to taking out the surgery specimen. Meanwhile, abdominal wall muscles are not damaged, surgical incisions are not enlarged, thereby being beneficial to the recovery of patients. At the same time, operation time is saved and pains of patients are reduced.

The invention discloses a single-port laparoscopic surgical instrument channel, and relates to the field of medical instruments. For overcoming the defects that an existing single-port laparoscopic surgical instrument channel cannot be well fixed to an incision part, surgical specimens are inconvenient to take out, and the price is high, the invention provides the single-port laparoscopic surgical instrument channel which comprises an upper-layer module and a lower-layer module. The upper end of the upper-layer module is provided with a smoke outlet, a gas inlet and three or more instrument channels. The lower-layer module is provided with a gas injection hole, an upper-layer chuck and a lower-layer chuck, the gas injection hole is located in the upper-layer chuck, and the upper-layer chuck is connected with the lower-layer chuck through a hollow supporting belt; gas is injected through the gas injection hole so that the hollow supporting belt can expand, the upper-layer chuck expands, the lower face of the upper-layer chuck makes close contact with the skin, the lower-layer chuck expands, and the upper face of the lower-layer chuck makes close contact with the peritoneum. The single-port laparoscopic surgical instrument channel has the beneficial effects that the single-port laparoscopic surgical instrument channel can be well fixed at an operative incision part, a specimen can be conveniently taken out, and the price is low.