An immortalized human liver cancer vascular endothelial cell line and its preparation method and application

A vascular endothelium and immortalization technology, applied in the biological field
CN105219732BActive Publication Date: 2018-06-29杨俐萍 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
杨俐萍
Publication Date
2018-06-29

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Abstract

The invention belongs to the field of biotechnology. The present invention provides an immortalized human liver cancer vascular endothelial cell line ECDHCC‑1, the preservation number of which is CCTCC NO: C2015172, which was preserved in China Center for Type Culture Collection on October 15, 2015. The invention also provides the construction method and identification of ECDHCC-1. The cell line obtained by the present invention has been subcultured by monoclonal subculture, and the subculture lasted for 18 months. There are 119 generations in total until the date of storage, and its biological characteristics remain consistent; The cumbersome procedures of "use now and take now" and the lack of resources for human liver cancer surgical specimens provide relevant researchers with a commercially stable tumor-derived vascular endothelial cell line, which is truly time-saving, labor-saving, efficient and convenient.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to the construction, in vitro culture system, identification and application of an immortalized human liver cancer vascular endothelial cell line. Background technique

[0002] Cancer is a major disease that seriously endangers human health, and the task of overcoming cancer and curing it is still very arduous. So far, although anticancer drugs have sprung up in the market, the problem of radical cure of cancer has not yet been solved. Studies have shown that tumor growth and metastasis are closely related to tumor blood vessels. In recent years, "starvation therapy" combined with chemotherapy has been used clinically to treat cancer, which is to block the neovascularization of tumors so that the supply of nutrients and oxygen required by tumor cells is lacking, resulting in starvation of cancer cells. However, anti-cancer drugs targeting blood vessels such as bevacizumab ...

Claims

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