5‑lipoxygenase pathway protein joint prediction kit for prognosis of patients with esophageal cancer

A technology of lipoxygenase and kit, which is applied in the field of 5-lipoxygenase pathway protein joint prediction kit for the prognosis of patients with esophageal cancer, can solve the problems of little research

Inactive Publication Date: 2017-12-05
CHIFENG UNIV +1
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Problems solved by technology

The expression of LTA4H in tumor tissues is poorly studied

Method used

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  • 5‑lipoxygenase pathway protein joint prediction kit for prognosis of patients with esophageal cancer
  • 5‑lipoxygenase pathway protein joint prediction kit for prognosis of patients with esophageal cancer
  • 5‑lipoxygenase pathway protein joint prediction kit for prognosis of patients with esophageal cancer

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Experimental program
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Embodiment Construction

[0018] We collected 297 surgical specimens of esophageal cancer patients from 2007 to 2011. The following is a further detailed description in conjunction with the embodiments of the drawings.

[0019] The steps of the immunohistochemical hypersensitivity two-step method are as follows.

[0020] After the specimen is serially sectioned, deparaffinized with xylene, and hydrated with gradient alcohol, the expression of the target protein is detected by the hypersensitive two-step method. The specific method is as follows.

[0021] (1) The slices are restored with 0.01M citrate repair solution, 3% H 2 O 2 After inactivating endogenous peroxidase and blocking with goat serum, they were incubated with 5-LO, FLAP, and LTA4H antibodies at 4°C overnight.

[0022] (2) The sections were incubated with Polymer enhancer and Polymer polymer and then developed by DAB.

[0023] (3) The sections were counter-stained with hematoxylin, dehydrated with gradient alcohol, and mounted with transparent xylen...

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Abstract

The invention is a kit for detecting the expression of paraffin-embedded surgical specimens of patients with esophageal cancer in combination with 5-lipoxygenase metabolic pathway protein antibodies, and then predicting the prognosis of patients. The kit includes antibodies ‑5‑LO, FLAP, and LTA4H antibodies for 3 target proteins, as well as goat serum, 0.01M citrate repair solution, 3% H2O2, Polymer enhancer, Polymer polymer, DAB chromogenic reagent, PBS solution. However, the kit of 5-lipoxygenase metabolic pathway protein used in the present invention to jointly predict the patient's prognosis has a higher predictive effect than single index detection, second only to the predictive ability of TNM staging, and the immune tissue based on the present invention The chemical hypersensitivity two-step method is a mature and reliable method that can be widely used in primary hospitals.

Description

Technical field [0001] The invention relates to a kit that combines 5-lipoxygenase pathway protein antibodies and uses an immunohistochemical hypersensitivity two-step method to detect the expression of samples of patients with esophageal cancer and predict the prognosis of the patients. Background technique [0002] Esophageal Squamous Cell Carcinoma (ESCC) is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors among Chinese. Of the more than 400,000 new cases of esophageal cancer in the world each year, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal cancer in the world. In the 1970s, a nationwide survey of malignant tumors was implemented, and Ci County in Hebei Province, Lin County in Henan Province, Yangquan County in Shanxi Province, Yanting in Sichuan Province, Chaoshan in Guangdong Province and Huai'an in Jiangsu Province we...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 白春英张俊毅史铁伟瑞云许秀娥吴健谊许丽艳李恩民
Owner CHIFENG UNIV
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