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Kit for detecting prognosis of patients with esophageal squamous cell carcinoma in Xinjiang region

A technique for esophageal squamous cell carcinoma and a kit, which is applied in the field of biology, can solve the problems of cumbersome methods for the prognosis effect of patients with esophageal squamous cell carcinoma, and achieve the effect of solving the lack of prognostic evaluation methods, rapid prediction methods and high prediction effect.

Inactive Publication Date: 2018-04-27
SHIHEZI UNIVERSITY
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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a test kit for detecting the prognosis of patients with esophageal squamous cell carcinoma in Xinjiang. cumbersome technical issues

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  • Kit for detecting prognosis of patients with esophageal squamous cell carcinoma in Xinjiang region
  • Kit for detecting prognosis of patients with esophageal squamous cell carcinoma in Xinjiang region
  • Kit for detecting prognosis of patients with esophageal squamous cell carcinoma in Xinjiang region

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Embodiment Construction

[0026] The present invention will be further described below through specific embodiments in conjunction with the accompanying drawings, but the present invention is not limited.

[0027] The 168 wax blocks of esophageal cancer tissues used in the examples of the present invention were all from the Department of Cardiothoracic Surgery, the First Affiliated Hospital of Shihezi University School of Medicine. This experiment was approved by the Ethics Committee of the First Affiliated Hospital of Shihezi University School of Medicine, and each patient signed an informed consent.

[0028] For immunohistochemical methods, refer to Identification of MXRA5 as a noveltissue biomarker for colorectal cancer progression by Wang Guanghui in 2013.

[0029] The graphics software used is SPSS 20.0.

[0030] The reagents used in the embodiments of the present invention are shown in Table 1, the main experimental instruments and consumables are shown in Table 2, and the reagents used in the e...

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Abstract

The invention provides a kit for detecting the prognosis of patients with esophageal squamous cell carcinoma in the Xinjiang region. The kit comprises a phospholipase epsilon 1 protein, an IKB alpha protein and a mutant P53 protein. The kit also comprises a citrate buffer solution with the content of 0.01M, an ethylenediaminetetraacetic acid repair solution with the content of 10mM, a H2O2 solution with the mass concentration of 3%, a phosphate buffer solution with the content of 0.01M, a BSA blocking solution with the mass concentration of 5%, an immunohistochemical Envision kit and a DAB developing reagent. The kit can forecast the prognosis of patients through detecting the expression case of the paraffin-embedded surgical specimen of the patients with esophageal squamous cell carcinomathrough PLCE1, IKB alpha and P53 proteins. Compared with the existing kit realizing single index detection, the kit has higher prediction effects and has the prediction ability similar to the TNM staged prediction ability. The two-step immunohistochemical method using the kit is a mature and reliable method that can be widely used in primary hospitals.

Description

technical field [0001] The invention belongs to the field of biology and relates to a detection kit, in particular to a kit for detecting the prognosis of patients with esophageal squamous cell carcinoma in Xinjiang. Background technique [0002] Esophageal cancer ranks ninth among the top ten common malignant tumors in humans. According to the 2012 global cancer epidemiological statistics (GLOBOCAN2012), there are 323,008 new cases of esophageal cancer and 281,217 deaths in the world. China is an area with a high incidence of esophageal cancer in the world, especially male patients, and the main tissue type is squamous cell carcinoma (referred to as esophageal squamous cell carcinoma). According to the latest data from the "Annual Report on Cancer Registration in China" published in 2012, esophageal squamous cell carcinoma ranks fifth in incidence and fourth in death among malignant tumors in my country, posing a serious threat to the quality of life and even life safety of...

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 崔晓宾禹洁彭昊韩雪平陈曦李军陈云昭李锋
Owner SHIHEZI UNIVERSITY
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