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Human non-small cell lung cancer cell line, and establishment method and application thereof

A non-small cell lung cancer, cell line technology, applied in the field of human non-small cell lung cancer cell line and its establishment, can solve the problem of large difference in biological characteristics, low success rate of squamous cell carcinoma cell line establishment, and human non-small cell lung cancer cell line. It is related to the problem of low biodiversity, and achieves the effects of stable drug resistance characteristics, high scientific research value and development significance, and high tumor formation rate.

Pending Publication Date: 2015-06-10
SHANGHAI CHEMPARTNER CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to solve the low biological diversity of existing human non-small cell lung cancer cell lines, especially the low success rate of establishment of squamous cell carcinoma cell lines, and at the same time, the biological characteristics of existing cell lines are quite different from those of clinical lung cancer To provide a new squamous cell line of human non-small cell lung cancer and its establishment method and application

Method used

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  • Human non-small cell lung cancer cell line, and establishment method and application thereof
  • Human non-small cell lung cancer cell line, and establishment method and application thereof
  • Human non-small cell lung cancer cell line, and establishment method and application thereof

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Embodiment 1

[0043] The preparation of embodiment 1LUXC-001 cell

[0044] A fresh resection specimen of clinical non-small cell lung cancer was obtained from Nantong Cancer Hospital (the clinical diagnosis of the specimen was lung non-small cell carcinoma: moderately differentiated squamous cell carcinoma), and fresh HBSS buffer (containing 500 U / ml penicillin G, 500 μg / ml streptomycin sulfate and 1.25μg / ml amphotericin B) after rinsing, cut into small pieces of 20-50 mg, subcutaneously puncture and inoculate in immunodeficiency animal SCID mice to obtain the parental tumor model LUX001; puncture inoculate non-small cell lung cancer After 70-90 days, the tumor-bearing animals were sacrificed, and the LUX001 subcutaneous tumor was cut into 1-2mm under sterile conditions 3 small pieces.

[0045] Primary culture method: Digest and resuspend the tumor tissue for 2 to 4 hours, stop the digestion, and collect the cells by sieve filtration; purify the cell suspension by gradient centrifugation ...

Embodiment 2

[0048] The biological characteristics and application of embodiment 2LUXC-001 cells

[0049] The invention adopts PC-1 culture medium to cultivate and purify LUXC-001 cells, so that they can grow and pass stably in vitro for a long time. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. According to experimental observation and verification, LUXC-001 grown in vitro has a typical epithelial-like morphology, the main body is paving stone-like, and grows in a clonal overlay, and can exhibit adhesive aggregation when the density is high. Genetic studies confirmed that the cells were aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The cells can form tumors in nude mice and have tumorigenicity. The correspo...

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Abstract

The invention discloses a non-small cell lung cancer cell line, and an establishment method and application thereof. The human non-small cell lung cancer cell line is preserved in China Center for Type Culture Collection with an accession number of CCTCC NO: C2012128. The establishment method comprises the following steps: step 1, acquiring a fresh clinical human non-small cell lung cancer surgical specimen, cutting the specimen into small blocks with a size of 20 to 50 mg and inoculation mammals with the block through subcutaneous puncture; and step 2, in 70 to 90 days after the puncture-inoculation, killing a tumor-bearing animal, taking tumor tissue out of the tumor-bearing animal and carrying out primary culture and subculture of cancer cells. According to the invention, the human non-small cell lung cancer cell line has the advantages of stable properties, capability of realizing stable multiple passage, a high tumor formation rate, a short latent period and good homogeneity; meanwhile, the human non-small cell lung cancer cell line has acquired drug resistance to gefitinib, can be used to analyze correlation between in-vitro and in-vivo drug sensibilities and drug resistance and is the ideal cell line for basic research on and preclinical application of human non-small cell lung cancer.

Description

technical field [0001] The invention belongs to the field of cell lines, in particular to a human non-small cell lung cancer cell line and its establishment method and application. Background technique [0002] In recent years, with the development of industry, the deterioration of people's living environment, the development of unhealthy lifestyles and the aging of society, the incidence of lung cancer has increased year by year. At present, lung cancer has become the first malignant tumor in my country. At the same time, lung cancer is one of the malignant tumors with the highest mortality rate in the world. Although its diagnosis and treatment level has been greatly improved in recent years, the 5-year survival rate is still lower than 15%. Therefore, there is still great urgency and space for lung cancer related research, and the establishment of new lung cancer cell lines is very important for the study of lung cancer carcinogenesis, invasion and metastasis, drug resist...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027A61K49/00C12Q1/02C12R1/91
Inventor 强福林汤旭蓁胡刚张一心许春花谢付波秦宵然
Owner SHANGHAI CHEMPARTNER CO LTD
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