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Myxococcus petiole preying on plant pathogenic bacteria and its application in biological control of bacterial diseases

A technology of plant pathogenic bacteria and pathogenic bacteria, applied in the field of applied microbiology, can solve problems such as differences in antibacterial effects

Active Publication Date: 2020-10-23
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are large differences in the antibacterial effects of different species of strains. For example, Bacillus shows different antibacterial properties in terms of resistance to plant pathogens, and related strains have also applied for patent protection (201380042989.6; 201410164779.0 and 201510666311.6, etc.)

Method used

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  • Myxococcus petiole preying on plant pathogenic bacteria and its application in biological control of bacterial diseases
  • Myxococcus petiole preying on plant pathogenic bacteria and its application in biological control of bacterial diseases
  • Myxococcus petiole preying on plant pathogenic bacteria and its application in biological control of bacterial diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Isolation and purification of strains

[0032] 1.1 After sample collection, air-dry naturally at room temperature (to reduce the contamination of mold and miscellaneous bacteria).

[0033] Soil samples were collected from Anhui and other places and air-dried naturally at room temperature.

[0034] 1.2 Induction of Myxobacter Fruiting Body

[0035] 1.2.1 Fecal particle induction plate method: put about 20 grams of air-dried soil samples in a petri dish, half-embed 3-4 sterilized rabbit feces on the surface of the soil samples, add 25 μg / ml of ether sterilized Nebendron (25mg / ml) or carbendazim (30mg / l), soaked for 6h, poured off the residual liquid, incubated at 30℃, and began to observe the formation of fruiting bodies on the fecal ball after 48h.

[0036] 1.2.2 Yeast induction plate method: take WCX (cycloheximide 25mg / ml) medium as the basal medium, pour it into a sterile plate, draw a cross line with yeast on the surface of the medium, and place a small am...

Embodiment 2

[0047] Example 2. Identification of strains

[0048] 2.1 Morphological features

[0049] The myxobacteria BS isolated and screened were negative by Gram staining, and observed under oil microscope by crystal violet staining. layer of mucus layer; observed by light microscope at 10×, it was found that the strain had a colony effect in the process of growth, and the colonies gathered together to form fruiting bodies at the later stage of growth ( figure 2 ).

[0050] 2.2 Identification

[0051] The 16S rDNA gene sequence of the strain was obtained by PCR amplification of the 16S rDNA of the strain, and the 16S rDNA sequence of the typical strain similar to the experimental strain was obtained by logging in to NCBI (www.ncbi.nlm.nih.govPblastP). The obtained sequencing results were compared with the known sequences in the database in the GenBank database using BLAST software, and the strain was preliminarily identified as Myxococcus stipitatus and named BS through sequence al...

Embodiment 3

[0052] Example 3. Research on the predation effect of Myxococcus stipitatus BS on phytopathogenic bacteria 3.1 Research on the predation effect of strain BS on various phytopathogenic bacteria.

[0053] Ea, Dickeya fangzhongdai, Dickeya chrysanthemi, Dickeya solani, Pcc, and RS were inoculated into LB liquid medium (tryptone 1%, NaCl 1%, yeast extract 0.5%), cultured at 30°C overnight, centrifuged at 8000 rpm for 3 min, and the cells were collected with Resuspend in sterile water to make it OD 600 is 10. It was suspended in TPM (10 mM Tris-HCl [pH 7.6], 1 mM KH2PO4 [pH 7.6], 8 mM MgSO 4 )) on a solid plate to dry before use. Strain BS (CCTCCM2017496) was inoculated into CYE liquid medium (Casitione 1%, Yaest extract 0.5% MgSO 4 ·7H 2 (00.1% pH 7.2-7.6), culture at 30°C for 2 days, centrifuge at 8000rpm for 3min, collect the cells, resuspend the cells with sterile water, and the cell concentration is 10 8 cells / l, hanging drop on the dried pathogenic bacteria moss, and cul...

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Abstract

The invention discloses a petiole myxococcus which preys on plant pathogenic bacteria and its application in biological control of bacterial diseases. A Myxobacterium BS strain antagonizing plant pathogenic bacteria, the preservation number is CCTCCNO.M2017496. Myxococcus petiole BS of the present invention inhibits the growth of various plant pathogenic bacteria such as cabbage soft rot fungus, calla lily soft rot fungus, tomato bacterial wilt, potato blackleg fungus and amylovora fungus by predating, showing Myxobacteria BS broad-spectrum antipathogenic bacterial capacity. Pot experiments show that myxobacteria BS can effectively inhibit the infection of potatoes and calla lilies by Pectobacterium carotovorum subsp. carotovorum, reduce the incidence of soft rot, and can be used for plant pathogenic bacteria biological control of plant diseases.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and relates to a strain of Myxococcus petioides that preys on plant pathogenic bacteria and its application in the biological control of bacterial diseases. Background technique [0002] Plant disease is one of the important constraints to high quality and high yield of crops. It is estimated that the average disease loss of major crops in the world accounts for nearly 14% of the total output, and the annual direct economic loss is as high as hundreds of billions of dollars. In order to deal with the harm caused by plant diseases, chemical pesticide control and crop breeding have become important means at present. However, due to the limitations of environmental pollution and long breeding cycle caused by chemical pesticides, environment-friendly biocontrol methods have become the focus of research. focus. Microbial biological control technology has the characteristics of high efficiency, no ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N63/20A01P1/00C05G3/00C12R1/01
CPCA01N63/00A01N63/10C05G3/00C12N1/20C12P1/04C12N1/205C12R2001/01
Inventor 崔中利李周坤王婷罗雪黄彦
Owner NANJING AGRICULTURAL UNIVERSITY
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