Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Preparation of magnetic silicon dioxide microsphere with metallic ion chelated surface and use thereof

A technology of silica and metal ions, which is applied in the field of materials for separating hexahistidine proteins, can solve problems such as metal ion omission, weak interaction force, and weak protein interaction force

Inactive Publication Date: 2008-12-17
SHAANXI NORMAL UNIV
View PDF0 Cites 32 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In common complexing agents, IDA is three-coordinated, and the force between it and metal ions is relatively weak, which can easily cause the problem of missing metal ions. N, N, N'-tris(carboxymethyl)ethylenediamine TED It is a five-coordinate, and has a strong interaction with metal ions. Since only one bond interacts with protein, the interaction with protein is too weak.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of magnetic silicon dioxide microsphere with metallic ion chelated surface and use thereof
  • Preparation of magnetic silicon dioxide microsphere with metallic ion chelated surface and use thereof
  • Preparation of magnetic silicon dioxide microsphere with metallic ion chelated surface and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides the preparation method of magnetic silica microspheres with surface chelated metal ions, and its process steps are as follows:

[0037] 1. Preparation of magnetic silica microspheres

[0038] Take 7.2892g of cetyltrimethylammonium bromide and 100.0g of toluene into a four-necked flask, the mass ratio of cetyltrimethylammonium bromide to toluene is 1:15, and use a mixer to Stir well; 0.329g FeCl 2 4H 2 O with 0.987g FeCl 3 ·6H 2 O was dissolved in 9.4397g of secondary water to prepare an aqueous solution with a concentration of 140g / L, FeCl 2 4H 2 O and FeCl 3 ·6H 2 O mass ratio is 1:3, at 2~3mL / min N 2 Add this solution dropwise to the above mixture at 4-5mL / h under air protection, and continue stirring at 800 rpm for 4-6 hours; then add 2mL of ammonia water with a mass concentration of 25%, cetyltrimethylammonium bromide The mass ratio of ammonia water with a mass concentration of 25% is 1:0.17, and the stirring is continued at 800 rpm...

Embodiment 2

[0047] In the preparation of magnetic silica microspheres process step 1, get 7.2892g cetyltrimethylammonium bromide and 87.47g toluene and join in the four-necked flask, cetyltrimethylammonium bromide and toluene The mass ratio is 1:12, stir evenly with a stirrer at 1000 rpm; mix 0.305g FeCl 2 4H 2 O with 0.916g FeCl 3 ·6H 2 O was dissolved in 9.4397g of secondary water to prepare an aqueous solution with a concentration of 130g / L, FeCl 2 4H 2 O and FeCl 3 ·6H 2 O mass ratio is 1:3, at 2~3mL / min N 2 Add this solution dropwise to the above mixture at 4-5mL / h under air protection, and continue stirring at 800 rpm for 4-6 hours; The mass ratio of ammonium to ammonia water with a mass concentration of 25% is 1:0.1, and the stirring is continued at 800 rpm for 2 hours. In the mixture, ferric oxide nanoparticles are formed; slowly add ethyl orthosilicate at 2 to 3 mL / h Ester 6.78mL, hexadecyltrimethylammonium bromide and tetraethyl orthosilicate in a mass ratio of 1:1, stir...

Embodiment 3

[0052] In the preparation of magnetic silica microspheres process step 1, get 7.2892g cetyltrimethylammonium bromide and 145.784g toluene and join in the four-necked flask, cetyltrimethylammonium bromide and toluene The mass ratio is 1:20, stir evenly with a stirrer at 1000 rpm; mix 0.354g FeCl 2 4H 2 O with 1.060g FeCl 3 ·6H 2 O was dissolved in 9.4397g of secondary water to prepare an aqueous solution with a concentration of 150g / L, FeCl 2 4H 2 O and FeCl 3 ·6H 2 O mass ratio is 1:3, at 2~3mL / min N 2 Add this solution dropwise to the above mixture at 4~5mL / h under air protection, and continue stirring at 800 rpm for 4~6 hours; The mass ratio of ammonium to 25% ammonia water is 1:0.2, and the mixture is continuously stirred at 800 rpm for 2 hours. In the mixture, iron ferric oxide nanoparticles are formed; slowly add ethyl orthosilicate at 2 to 3 mL / h Esters 11.76mL, hexadecyltrimethylammonium bromide and tetraethyl orthosilicate at a mass ratio of 1:1.5, stirred and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Saturation magnetizationaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for preparing magnetic silicon dioxide microspheres with the surfaces chelated with metal ions; the method of the inventioncomprises the following steps of preparation of the magnetic silicon dioxide microspheres, epoxidation of the magnetic silicon dioxide microspheres, carboxylation of the magnetic silicon dioxide microspheres and chelation technique of metal ions. Compared with the existing preparing method, the method of the invention has the advantages of reasonable design, simple operation and large amount of chelation of metal, etc. The magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions, which are prepared by adopting the method, contain cobalt ions, have stronger bonding force with the modified microspheres so as to lead the cobalt ions to be not easy to leak out, and have higher selectivity and proper bonding strength with histidine on the protein, thus leading the magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions to have high selectivity to hexameric histidine tag protein, and being easy to realize the separation and the purification of the target protein; the magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions, which are prepared by adopting the method, can be used for separating the hexameric histidine tag protein.

Description

technical field [0001] The invention belongs to the technical field of materials, and in particular relates to a material used for isolating hexahistidine protein from Escherichia coli. Background technique [0002] Biomagnetic separation technology is a high-efficiency separation technology developed on the basis of traditional magnetic separation technology, which is applied to organisms such as cells, bacteria, nucleic acids and proteins. It uses the characteristics of magnetic or magnetically labeled organisms to perform qualitative movements under the action of an external magnetic field to extract, enrich, separate and purify target organisms. Early work mainly focused on the development of preparation techniques for magnetic markers, including the adsorption of magnetic nanoparticles to polymer microspheres such as agarose, polyacrylamide, polyglutaraldehyde, and polyvinylaldehyde by immersion in ferrofluid. The microspheres are characterized by a uniform particle si...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C01B33/18C07K1/13
Inventor 冯国栋胡道道崔亚丽杨柳杨磊
Owner SHAANXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com