The invention provides
gelatin derivative microspheres coated with
stem cell exosomes, a preparation method of the
gelatin derivative microspheres and application of the
gelatin derivative microspheres in preparation of external
ophthalmic preparations, and relates to the technical field of medicines. The gelatin derivative
microsphere mainly comprises a
microsphere core and a shell, wherein the
microsphere core is a
stem cell exosome coated with a gelatin derivative; the shell is obtained by crosslinking a gelatin derivative and
sodium hyaluronate. The gelatin derivative used in the invention not only has good compatibility with the
stem cell exosome, but also has good dispersibility in
tears or periorbital
secretion after being prepared by crosslinking the gelatin derivative and
sodium hyaluronate, so that the
elimination of an
eye protection mechanism to the microspheres can be reduced, and the slow release effect of the
exosome can be improved; besides, the shell formed by the
hyaluronic acid has a very good tackifying effect, so that the
retention time of the gelatin derivative
hyaluronic acid microspheres in eyes is prolonged, and the problem of burst release of the
medicine is also avoided by virtue of the double-layer coated microsphere structure.