Supramolecular hydrogel gene vector material, and preparation method and application thereof

A technology of supramolecular hydrogel and gene carrier, applied in the field of biomedical engineering materials, can solve the problems that the application of supramolecular hydrogel has not been reported yet, and achieve convenient and fast application, good shear thinning, low concentration and temperature Effect
CN101716346AInactive Publication Date: 2010-06-02SUN YAT SEN UNIV
0 Cites 13 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2010-06-02
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a supramolecular structural hydrogel gene vector material and a preparation method and application thereof. The method comprises the following operation steps: synthesizing a multi-block copolymer with cation chain segments through modification of a terminal group of triblock copolymer of a polyethylene glycol-polypropylene glycol-polyethylene glycol which is modified by polylysine; then, mixing the copolymer with DNA solution to obtain DNA compound micelle; further, mixing and then stirring the micelle solution and solution of alpha-cyclodextrin; and keeping mixed solution at the room temperature to obtain hydrogel. The hydrogel can be used for preparing an injectable gene vector. The method has the advantages that the method has simple operation, can adjust the strength of the hydrogel and the gelatination time, can mold at the room temperature and does not relate to chemical crosslinking reaction and the use of organic solvent; and the obtained hydrogel has the advantages of temperature sensitivity, good bio-compatibility, obvious transfection effect and the like; and the method is hopeful to be widely applied in the field of a biomedical engineering material.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to a supramolecular hydrogel which can be used as a gene carrier and its preparation method and application. Background technique

[0002] Gene therapy is an effective method currently used in the treatment of cancer and innate immune system diseases. The key to the implementation of this technology is to select the appropriate gene carrier and gene introduction method, so that the gene can be expressed in cells safely, efficiently, controllably and stably. As a class of biomedical materials that can be used as drug carriers, hydrogels have the characteristics of high loading rate, protection of macromolecular drugs from degradation or removal by organisms, and sustainable release of drug molecules. Research for gene loading and controlled release. For example, Park et al. used double-bond-modified polyethylene glycol-polypropylene glycol-polyethylene gl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More