Phaseolus vulgaris alpha-amylase inhibitor extraction and separation method

The technology of an amylase inhibitor and a separation method, which is applied in the field of extraction and separation of white kidney bean alpha-amylase inhibitors, can solve the problem of high cost, and achieve the effects of increasing yield, inhibiting hydrolysis reaction and light color.

Inactive Publication Date: 2015-07-15
SHAANXI XINMAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them are used in scientific research or expensive medicines, but they cannot be used in food and health care products because of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] White kidney beans are crushed, passed through a 10-mesh sieve, weighed 50 kg of white kidney bean coarse powder, added 10 times of tap water adjusted to pH 7.5 with aqueous sodium bicarbonate solution, stirred and extracted at 20°C for 4 hours, filtered, and the filtrate was collected for the first time. Add 8 times of tap water with sodium bicarbonate aqueous solution to adjust the pH to 7.5 to the first residue, stir and extract at 20°C for 2 hours, filter, collect the second filtrate, add sodium bicarbonate aqueous solution to the second residue to adjust the pH to 7.5 8 times of tap water, stirred and extracted at 20°C for 2h, filtered, collected the third filtrate, combined the three filtrates to form a mixed filtrate, and concentrated the mixed filtrate at 65°C under reduced pressure to form a concentrate, which was the mixture 3 times the amount of the raw material of the filtrate, heat and boil the concentrated solution for 5-10min, add 1% gluconolactone in the ...

Embodiment 2

[0029] White kidney beans are crushed, passed through a 10-mesh sieve, weighed 50 kg of white kidney bean coarse powder, added 15 times of tap water adjusted to pH 7.5 with aqueous sodium bicarbonate solution, stirred and extracted at 20°C for 6 hours, filtered, and the filtrate was collected for the first time. Add 12 times of tap water with sodium bicarbonate aqueous solution to adjust the pH to 7.5 to the first residue, stir and extract at 20°C for 4 hours, filter, collect the second filtrate, add sodium bicarbonate aqueous solution to the second residue to adjust the pH to 7.5 tap water 12 times, stirred and extracted at 20°C for 4h, filtered, collected the third filtrate, combined the three filtrates to form a mixed filtrate, and concentrated the mixed filtrate under reduced pressure at 65°C to form a concentrate, which was the mixture 3 times the amount of the raw material of the filtrate, heat and boil the concentrated solution for 5-10min, add 1% gluconolactone in the c...

Embodiment 3

[0031] White kidney beans are crushed, passed through a 10-mesh sieve, weighed 50 kg of white kidney bean coarse powder, added 15 times of tap water adjusted to pH 7.5 with aqueous sodium bicarbonate solution, stirred and extracted at 35°C for 6 hours, filtered, and the filtrate was collected for the first time. Add 12 times of tap water with sodium bicarbonate aqueous solution to adjust the pH to 7.5 to the first residue, stir and extract at 35°C for 4 hours, filter, collect the second filtrate, add sodium bicarbonate aqueous solution to the second residue to adjust the pH to 7.5 tap water 12 times, stirring and extracting at 35°C for 4h, filtering, collecting the third filtrate, combining the three filtrates to form a mixed filtrate, and concentrating the mixed filtrate at 65°C under reduced pressure to form a concentrate, which is the mixture 3 times the amount of the raw material of the filtrate, heat and boil the concentrated solution for 5-10min, add 1% gluconolactone in ...

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Abstract

The present invention provides a phaseolus vulgaris alpha-amylase inhibitor extraction separation method, and relates to the field of food science biological active component extraction and separation, wherein the obtaining rate can be increased, and the yield can be improved. The method comprises: crushing phaseolus vulgaris to obtain phaseolus vulgaris crude powder, carrying out stirring extraction on the phaseolus vulgaris crude powder 3 times with water at a room temperature, filtering to obtain a filtrate, carrying out pressure reducing concentration on the filtrate to obtain a concentrated solution, carrying out boiling sterilization on the concentrated solution, adding a glucono delta-lactone coagulant, precipitating, filtering to obtain a precipitate, and sequentially carrying out decolorizing, dewatering, filtration draining, vacuum drying, and crushing so as to obtain the phaseolus vulgaris alpha-amylase inhibitor product.

Description

technical field [0001] The invention relates to the field of extraction and separation of bioactive ingredients in food science, in particular to a method for extraction and separation of white kidney bean α-amylase inhibitors. Background technique [0002] The white kidney bean a-amylase inhibitor is extracted from the white kidney bean (phaseolus coccineus L., P. multiflorus willd.) of the leguminous family Kidney Bean. The biological name of white kidney bean is multi-flowered bean. It is native to Mexico and Argentina in the Americas. After artificial cultivation and domestication, it has adapted to the cold and humid plateau. The countries with large planting areas are Argentina, the United States, and Mexico in the Americas, the United Kingdom in Europe, China, and Japan in Asia. Now it is planted in various provinces and regions in China, and the provinces with larger areas are Yunnan, Guizhou, and Sichuan. [0003] White kidney bean a-amylase inhibitor is a natural...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/20A61K36/48A61P3/10
Inventor 李多伟刘长霞
Owner SHAANXI XINMAO BIOTECH CO LTD
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