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Industrial preparation method of safe and efficient white kidney bean alpha-amylase inhibitor

A technology of amylase inhibitor and white kidney bean, which is applied in the field of preparation of plant active ingredients, can solve the problem that it is not suitable for the large-scale industrial production of white kidney bean α-amylase inhibitor, and the removal of lectin and trypsin inhibitor is not considered 、 It is difficult to guarantee the safety and effectiveness of white kidney bean α-amylase inhibitor products

Active Publication Date: 2015-08-26
无锡华康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The preparation methods of many white kidney bean α-amylase inhibitors reported at present, or do not consider the removal of lectin and trypsin inhibitors, it is difficult to ensure the safety and effectiveness of white kidney bean α-amylase inhibitor products, or use Salting out, dialysis, chromatographic separation and other processes are not suitable for industrialized large-scale production of white kidney bean α-amylase inhibitors

Method used

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  • Industrial preparation method of safe and efficient white kidney bean alpha-amylase inhibitor
  • Industrial preparation method of safe and efficient white kidney bean alpha-amylase inhibitor
  • Industrial preparation method of safe and efficient white kidney bean alpha-amylase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] White kidney beans are crushed, and the bean flour that has been collected through a 50-mesh sieve;

[0062] Mix 100g soybean powder with 500mL distilled water, adjust the pH to 8.0 with 1mol / L NaOH solution, stir and extract at room temperature for 2h;

[0063] The mixture was centrifuged at 4°C, 8000r / min for 40min, and the supernatant was collected;

[0064] Use 1mol / L HCl solution to adjust the pH of the supernatant to 3.0, inactivate the enzyme in a water bath at 60°C for 20 minutes, and centrifuge (5000r / min, 10min) to take the supernatant;

[0065] Adjust the pH of the supernatant to 8.0 with 1mol / L NaOH solution, stir at room temperature for 2 hours, adjust the pH of the supernatant to 6.9 with 1mol / L HCl solution, and centrifuge (5000r / min, 10min) to take the supernatant;

[0066] The supernatant was ultrafiltered, and the pore size of the ultrafiltration membrane used was 10kDa, and the retentate was collected;

[0067] The retentate is freeze-dried and ultr...

Embodiment 2

[0070] White kidney beans are crushed, and the bean flour that has been collected through a 40-mesh sieve;

[0071] Mix 100g of soybean powder with 500mL of distilled water, adjust the pH to 9.0 with 1mol / L NaOH solution, stir and extract at room temperature for 2h;

[0072] The mixture was centrifuged at 4°C, 8000r / min for 60min, and the supernatant was collected;

[0073] Use 1mol / L HCl solution to adjust the pH of the supernatant to 5.0, inactivate the enzyme in a water bath at 60°C for 20 minutes, and centrifuge (5000r / min, 10min) to take the supernatant;

[0074] Adjust the pH of the supernatant to 9.0 with 1mol / L NaOH solution, stir at room temperature for 2 hours, adjust the pH of the supernatant to 6.9 with 1mol / L HCl solution, and centrifuge (5000r / min, 10min) to take the supernatant;

[0075] The supernatant was ultrafiltered, and the pore size of the ultrafiltration membrane used was 20kDa, and the retentate was collected;

[0076] The retentate is spray-dried and...

Embodiment 3

[0079] White kidney beans are crushed, and the bean flour that has been collected through a 60-mesh sieve;

[0080] Mix 100g soybean powder with 500mL distilled water, adjust the pH to 10.0 with 1mol / L NaOH solution, stir and extract at room temperature for 2h;

[0081] The mixture was centrifuged at 4°C, 8000r / min for 30min, and the supernatant was collected;

[0082] Use 1mol / L HCl solution to adjust the pH of the supernatant to 4.0, inactivate the enzyme in a water bath at 60°C for 20 minutes, and centrifuge (5000r / min, 10min) to take the supernatant;

[0083] Use 1mol / L NaOH solution to adjust the pH of the supernatant to 10.0, stir at room temperature for 2 hours, use 1mol / L HCl solution to adjust the pH of the supernatant to 6.9, and centrifuge (5000r / min, 10min) to take the supernatant;

[0084] The supernatant was ultrafiltered, and the pore size of the ultrafiltration membrane used was 30kDa, and the retentate was collected;

[0085] The retentate is freeze-dried an...

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Abstract

The invention discloses an industrial preparation method of a safe and efficient white kidney bean alpha-amylase inhibitor and belongs to the field of preparation of active plant ingredients. The industrial preparation method of the safe and efficient white kidney bean alpha-amylase inhibitor comprises the following steps: firstly grinding white kidney beans into powder, extracting by alkaline water, then adjusting supernatant liquor prepared by centrifuging till the supernatant liquor is acidic, carrying out enzyme deactivation, alkali treatment and ultra-filtration to obtain a retention liquid, and drying the retention liquid to obtain the white kidney bean alpha-amylase inhibitor product free of phytolectins and trypsin inhibitors. The alpha-amylase inhibitor product prepared by the method is free of activity of phytolectins and trypsin inhibitors; the alpha-amylase inhibitor product is high in activity, so that the white kidney bean alpha-amylase inhibitor product is safe and efficient; meanwhile, the industrial preparation method of the safe and efficient white kidney bean alpha-amylase inhibitor is simple in process and low in cost, is applicable to industrial large-scale production, and has high economic and social benefits.

Description

technical field [0001] The invention relates to a safe and efficient industrial preparation method of white kidney bean α-amylase inhibitor, which belongs to the field of preparation of plant active ingredients. Background technique [0002] With the rapid development of social economy, diabetes is becoming more and more popular, and it has shown a trend of younger age. Diabetes mellitus is a systemic disease mainly characterized by disorders of glucose metabolism, and one of its typical features is postprandial hyperglycemia. The continuous rise of blood sugar after a meal will produce glucose toxicity, damage the cardiovascular system, and eventually cause irreversible damage to the human body. Therefore, controlling postprandial blood sugar rise is crucial for controlling and delaying the onset and development of diabetes. White kidney bean α-amylase inhibitor is a glycoprotein that can specifically inhibit human saliva and intestinal amylase activity. It can reduce pos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P3/10A61P3/04
Inventor 赵伟让一峰杨瑞金
Owner 无锡华康生物科技有限公司
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