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Solid mixture containing alpha-amylase and its prepn process and application in pharmaceutical industry

The technology of an amylase inhibitor and mixture is applied in the field of solid mixture, and achieves the effects of simple process operation, easy production and amplification, and not easy to absorb moisture

Active Publication Date: 2006-05-17
南京生命原健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on adding sugar alcohols to the crude extract of α-amylase inhibitors to maintain the activity of α-amylase inhibitors after drying

Method used

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  • Solid mixture containing alpha-amylase and its prepn process and application in pharmaceutical industry
  • Solid mixture containing alpha-amylase and its prepn process and application in pharmaceutical industry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1α-amylase inhibitor and mannitol spray drying mixture

[0039] 1. Crude extraction of alpha-amylase inhibitors.

[0040]●Weigh 100kg of flour, add 400L, 0.1mol / L NaCl solution, put it in a stirring tank with a rotation speed of 100rpm / min and stir and extract for 3 hours, centrifuge the extract with a tubular centrifuge to obtain a clear liquid of 365L, and measure the protein concentration It is 2.39mg / ml, and the inhibition specific activity is 41.3U / mgpro.

[0041] ●The centrifugal supernatant was concentrated to a volume of 270 liters with an ultrafiltration membrane with a cut-off molecular weight of 6000, the measured protein concentration was 5.61 mg / ml, and the inhibition specific activity was 56.9. The ultrafiltration concentrate was salted out with ammonium sulfate of 25% saturation and centrifuged to obtain a solid, then dissolved with 0.1mol / ml phosphate buffer and centrifuged again to obtain 100L of clear liquid, the recorded...

Embodiment 2

[0056] Preparation of Example 2 α-amylase inhibitor and xylitol spray-dried mixture

[0057] 1. The crude extract of α-amylase inhibitor: as in Example 1.

[0058] 2. Preparation of α-amylase inhibitor and xylitol spray-dried mixture

[0059] Take 10L of the crude extract of α-amylase inhibitor, add xylitol according to 5% of the weight of the crude extract of α-amylase inhibitor, stir evenly, spray dry at 70-180°C, and control the flow rate at 20-120ml / min, compressed air 0.1-0.4MPa. Thereby the α-amylase inhibitor product is obtained.

[0060] 3. The test results are as follows:

[0061] The protein content of the dry powder obtained by direct spray drying without adding xylitol is 20%, and the inhibition specific activity is 43.6U / mgpro;

[0062] Add xylitol at a weight ratio of 5% and then spray-dry the product: the protein content is 17.8%, and the inhibition specific activity is 71.9U / mgpro.

Embodiment 3

[0063] The preparation of embodiment 3α-amylase inhibitor and sorbitol spray drying mixture

[0064] 1. The crude extract of α-amylase inhibitor: as in Example 1.

[0065] 2. Preparation of α-amylase inhibitor and sorbitol spray-dried mixture

[0066] Take 10L of the crude extract of the α-amylase inhibitor, add sorbitol according to 70% of the weight of the crude extract of the α-amylase inhibitor, stir evenly, spray dry at 70-180°C, and control the flow rate at 20-120ml / min, compressed air 0.1-0.4MPa. Thereby the α-amylase inhibitor product is obtained.

[0067] 3. The test results are as follows:

[0068] The protein content of the dry powder obtained by direct spray drying without adding sorbitol is 20%, and the inhibition specific activity is 43.6U / mgpro;

[0069] After adding sorbitol in a weight ratio of 70%, spray-dried product: the protein content is 0.8%, and the inhibition specific activity is 82.9U / mgpro.

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Abstract

The present invention discloses one kind of solid mixture containing alpha-amylase and its preparation process and application in preparing medicine. The solid mixture is prepared through preparing coarse extract of alpha-amylase inhibitor via conventional process; adding sugar alcohol in the weight ratio to the coarse extract of alpha-amylase inhibitor organic 0.1-99.9 % to the coarse extract of alpha-amylase inhibitor via stirring and drying to obtain the mixture. The mixture may be used in preparing medicine for preventing and treating obesity, lipomatosis, atherosclerosis, hyperlipemia and diabetes. The mixture can maintain the bioactivity of alpha-amylase inhibitor with less moisture absorption and high stability. The preparation process is simple and easy use in industrial production.

Description

technical field [0001] The invention relates to a solid mixture containing an α-amylase inhibitor, and also relates to a preparation process and application of the mixture in pharmacy. Background technique [0002] α-amylase inhibitor is a pure natural biologically active substance extracted from wheat flour, which belongs to a kind of glycohydrolase inhibitor, exists in the endosperm of plant seeds, and is called "starch blocker" abroad. α-amylase inhibitor exerts weight loss effect through the inhibition of amylase, and is excreted from the body through the gastrointestinal tract, so it does not need to enter the blood circulation system, does not act on the brain center, and does not suppress appetite while losing weight, and the dosage is very high There is no side effect at any time, and it is very similar to the weight loss mechanism of Roche Xenical, which is in line with the weight loss principles of the World Health Organization. Because α-amylase inhibitors can in...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P3/04A61P3/06A61P3/10
Inventor 应汉杰吕浩徐国梅魏秀丽吴永宏赵谷林陈晓春欧阳平凯
Owner 南京生命原健康科技有限公司
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