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Method for processing deproteinized rice and product and for jointly preparing alpha-amylase inhibitor and protein peptide

A combined preparation technology of amylase inhibitors, applied in the field of preparation of deproteinized rice products, can solve the problems of complex extraction process and undisclosed separation of α-amylase inhibitors

Inactive Publication Date: 2019-12-17
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural α-amylase inhibitors derived from plants have the characteristics of structural diversity, low toxicity, and wide range of sources. Therefore, screening new α-amylase inhibitors with small side effects from plants has always been a research hotspot. At present, wheat, α-amylase inhibitors are isolated from the seeds of barley, sorghum, grains, peanuts, soybeans, taro, wild amaranth, etc., but there is no disclosure of α-amylase inhibitors isolated from rice
[0007] In addition, in the existing rice deproteinization process, only different treatments are performed on rice, and finally rice suitable for a certain special population is obtained.
For other nutrients in rice, such as the extraction of protein peptides, a special process is used for separate research, and the extraction process is complicated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 provides a method for jointly preparing deproteinized rice and products of α-amylase inhibitors and protein peptides. The raw material rice is rice with amylose accounting for more than 20% of the starch. The specific preparation steps are as follows:

[0034] (1) After cleaning the rice raw material, use ozone water with an ozone concentration of 0.5 mg / L, soak it at room temperature for 30 minutes, wash it with water once, continue to use a 0.6% aqueous solution of food grade citric acid, soak it at room temperature for 15 minutes, and wash it with water 2 times;

[0035] (2) Add pure water to the sterilized rice in step (1) in a ratio of 1:3 by rice water weight, then add 1% pepsin by rice weight, and add 10% pepsin per ton of enzymolysis solution. 11 The number of bacteria in CFU / ton adds acetic acid bacteria to the enzymatic hydrolysis solution, and carries out hydrolysis and fermentation under the conditions of initial pH 4 and temperature 37°C. The pH i...

Embodiment 2

[0041] Example 2 provides a method for jointly preparing deproteinized rice and products of α-amylase inhibitors and protein peptides. The raw material rice is rice with amylose accounting for more than 20% of the starch. The specific preparation steps are as follows:

[0042] (1) rice is cleaned, adopt chlorine dioxide concentration 30mg / L, soak 25min at room temperature, clean, then use acetic acid concentration 0.3%, soak 20min at room temperature, clean;

[0043] (2) Add pure water to the washed rice at a weight ratio of 1:1.5 to rice water, then add 0.5% flavor protease by rice weight, and carry out hydrolysis at a pH of 6 and a temperature of 55° C. The hydrolysis time is 15 hours. During the hydrolysis process, the pH is adjusted to 6 by the adjustment tank of the tank external circulation system every 1 hour. After the hydrolysis is completed, the material and liquid are separated, and the solid and liquid are collected separately;

[0044] (3) Heat the liquid collecte...

Embodiment 3

[0048] Example 3 provides a method for jointly preparing deproteinized rice and products of α-amylase inhibitors and protein peptides. The raw material rice is amylose accounting for more than 20% of broken rice. The specific preparation steps are as follows:

[0049] (1) Wash the broken rice, grind it, add pure water according to the ratio of material to liquid ratio 1:1.2, and add chlorine dioxide concentration to reach 20mg / L, soak at room temperature for 20min, filter, wash with pure water, drain the water ;

[0050] (2) Add pure water to the rice slurry after cleaning by rice water weight ratio 1:3, add 3.350 acid protease of rice weight 1.0% in reaction solution, add 0.5% glucose and sucrose (weight ratio 1:1) Mixed sugar, at the same time, 10 per ton of enzymatic solution 12 The number of bacteria in CFU / ton is added to the enzymolysis solution by adding complex bacteria of acetic acid bacteria and lactic acid bacteria (the ratio of the number of bacteria is 1:2) to fe...

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Abstract

The invention provides a method for preparing deproteinized rice and a product and for jointly preparing an alpha-amylase inhibitor and a protein peptide. The preparation method specifically comprisesthe following steps: washed rice or rice flour is used as a raw material; after sterilization, first enzymolysis is carried out by using an enzymatic hydrolysate to prepare alpha-amylase inhibitory peptide; after a solid material obtained by the first enzymolysis is sterilized, second enzymolysis is conducted and a second enzymatic hydrolysate is used to prepare protein peptide; a solid materialobtained by the second enzymolysis is directly steamed, dried and dispersed to obtain deproteinized rice or rice flour containing resistant starch, or is used to prepare box lunch; specific protease is added during the first enzymolysis, or zymophyte and specific protease are simultaneously added to perform enzymolysis and fermentation; and non-specific protease and triglyceride lipase are added during the second enzymolysis. According to the invention, contents of protein, sodium, potassium and phosphorus in rice are reduced, the alpha-amylase inhibitor and the antioxidant and ACEI-activity protein peptide are jointly prepared, and the content of resistant starch in deproteinized rice is increased.

Description

technical field [0001] The invention relates to a method for preparing rice, in particular to a method for preparing a deproteinized rice product that combines an α-amylase inhibitor and a protein peptide. Background technique [0002] Epidemiological studies have shown that my country is a country with a high incidence of chronic diseases, and the incidence of chronic kidney disease in adults is 10.8%. According to the "Report on the Status of Nutrition and Chronic Diseases of Chinese Residents (2015)", the overweight and obesity rates of adults in 2012 were 11.9% and 9.6% respectively; the overweight and obesity rates of children and adolescents aged 6-17 were 30.1% and 6.4% respectively . In 2012, among adults aged 18 and above, the prevalence of hypertension was 25.2%, and the prevalence of diabetes was 9.7%. At present, there are about 110 million diabetic patients in our country, and there is an upward trend. Those with abnormal glucose tolerance are as high as 15.5%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L7/10A23L5/20A23L33/00
CPCA23L7/10A23L5/276A23L33/00A23V2002/00
Inventor 刘良忠王梦颖王杨阳胡艺涵周雪琴
Owner WUHAN POLYTECHNIC UNIVERSITY
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