Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing highly-pure Porphyra yezoensis polysaccharide alpha-amylase inhibitor

An amylase inhibitor, a technology of laver striatellus, applied in the field of preparation of plant active ingredients, can solve the problems of incomplete removal of lectin, intolerant of high temperature treatment, ecological environment impact, etc., so as to prevent and manage type 2 diabetes, Good stability and high biological activity

Active Publication Date: 2018-03-23
JIANGNAN UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Proteins and peptides have high α-AI activity but incomplete removal of lectin, or the need for acid-base conditions in the extraction process will seriously affect the ecological environment, and they are not resistant to high temperature treatment, and in severe cases, anaphylactic shock, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing highly-pure Porphyra yezoensis polysaccharide alpha-amylase inhibitor
  • Method for preparing highly-pure Porphyra yezoensis polysaccharide alpha-amylase inhibitor
  • Method for preparing highly-pure Porphyra yezoensis polysaccharide alpha-amylase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Preparation of polysaccharide α-amylase inhibitor of Porphyra zebra

[0047] Pulverize Porphyra zebra, pass through an 80-mesh sieve, and collect Porphyra zebra powder;

[0048] Take 50g laver powder and add 1000mL deionized mixture, stir and extract at 70°C for 2h;

[0049] Centrifuge the extract at 4°C, 8000r / min for 20min, and collect the supernatant;

[0050] Use 1mol / L HCl solution to adjust the pH of the supernatant to 3.0, add 1% protease to enzymatically hydrolyze the miscellaneous protein, stir at 60°C for 2 hours, then use 1mol / L NaOH solution to adjust the pH of the enzymatic hydrolyzate to 6.5, inactivate the enzyme in a water bath at 80°C for 20min, and centrifuge Centrifuge at 8000r / min for 20min, and take the supernatant;

[0051] The supernatant is ultrafiltered, and the pore size of the ultrafiltration membrane is 10kDa, and the retentate is collected;

[0052]The retentate is spray-dried to obtain a powdered α-amylase inhibitor product...

Embodiment 2

[0053] Embodiment 2: Preparation of polysaccharide α-amylase inhibitor of Porphyra zebra

[0054] Pulverize Porphyra zebra, pass through a 60-mesh sieve, and collect Porphyra zebra powder;

[0055] Take 50g laver powder and add 1000mL deionized mixture, stir and extract at 50°C for 2h;

[0056] Centrifuge the extract at 4°C, 8000r / min for 20min, and collect the supernatant;

[0057] Use 1mol / L NaOH solution to adjust the pH of the supernatant to 8.0, add 2% protease to enzymolyze the miscellaneous protein, stir at 50°C for 2h, adjust the pH of the enzymolysis solution to 7.5 with 1mol / L HCl solution, and inactivate the enzyme in a water bath at 80°C for 20min. Centrifuge at 8000r / min for 20min, and take the supernatant;

[0058] The supernatant was ultrafiltered, and the pore size of the ultrafiltration membrane was 10kDa, and the retentate was collected;

[0059] The retentate is spray-dried to obtain a powdered α-amylase inhibitor product.

[0060] An appropriate amount ...

Embodiment 3

[0061] Embodiment 3: Preparation of polysaccharide α-amylase inhibitor of Porphyra zebra

[0062] Pulverize Porphyra zebra, pass through an 80-mesh sieve, and collect Porphyra zebra powder;

[0063] Take 50g laver powder and add 1500mL deionized mixture, stir and extract at 60°C for 2h;

[0064] Centrifuge the extract at 4°C, 8000r / min for 20min, and collect the supernatant;

[0065] Use 1mol / L HCl solution to adjust the pH of the supernatant to 5.0, add 4% protease to enzymatically hydrolyze the miscellaneous protein, stir at 40°C for 2 hours, then use 1mol / L NaOH solution to adjust the pH of the enzymatic hydrolyzate to 7.0, inactivate the enzyme in a water bath at 60°C for 20min, and centrifuge Centrifuge at 8000r / min for 20min, and take the supernatant;

[0066] The supernatant was ultrafiltered, and the pore size of the ultrafiltration membrane was 1kDa, and the retentate was collected;

[0067] The retentate is spray-dried to obtain a powdered α-amylase inhibitor prod...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a highly-pure Porphyra yezoensis polysaccharide alpha-amylase inhibitor, and belongs to the field of preparation of effective components in plants. The method comprises the following steps: milling Porphyra yezoensis, sieving the milled Porphyra yezoensis, performing water extraction, adding a supernatant obtained by centrifugation into protease to carry out enzymatic hydrolysis, performing enzyme deactivation, performing ultrafiltration on a supernatant obtained by centrifugation, and performing spray drying on the obtained retentate to obtain the Porphyra yezoensis polysaccharide alpha-amylase inhibitor product. The alpha-amylase inhibitor prepared in the invention has the advantages of non-toxicity, high purity, high vitality, good safety,high efficiency, simple process, low cost and good economic and social benefits.

Description

technical field [0001] The invention relates to a preparation method of a high-purity Porphyra zebra polysaccharide alpha-amylase inhibitor, which belongs to the field of preparation of plant active ingredients. Background technique [0002] Porphyra yezoensis algal body is oval, long oval, purple or slightly green. Seaweed is rich in vitamins, minerals, proteins, fatty acids, seaweed polysaccharides and other nutrients and physiologically active substances. Porphyra is one of the seaweeds with the richest polysaccharide content. The polysaccharide content in laver varies with the type of algae, growth time, and location. Usually, the polysaccharide content is 20%-40%. [0003] Diabetes is one of the major diseases that seriously threaten human life and health in the 21st century. Its incidence is related to factors such as genetic factors, lifestyle, diet structure and age, and it is a common metabolic disease. Epidemiological studies in recent years have shown that post...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61P3/10A23L33/125
CPCA23L33/125A23V2002/00C08B37/0003A23V2200/328
Inventor 赵伟曾傲琼杨瑞金
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com