Extraction process for amylase inhibiting protein

Abstract

The invention relates to an extraction process for amylase inhibitor. A kidney bean as a raw material bean is pulverized to be ultrathin, the powdered kidney bean is subjected to primary extraction in de-ionized water, then the primary extract is separated, filtered and dried, and then the primary extract is extracted in a supercritical carbon dioxide device. By adopting the process to extract amylase inhibiting protein, the yield of a finished product can achieve 14 to 19 percent, the cost is lower, the process period is shorter, and the product is safe and environment-friendly.

Description

technical field [0001] The invention relates to an extraction process of amylase inhibitors, in particular to a process for extracting amylase inhibitory proteins from kidney beans. Background technique [0002] Most carbohydrates in the diet are starch, which is hydrolyzed into maltose by amylase in saliva and pancreatic juice in a short period of time, and then absorbed in the intestinal tract in the form of glucose. The α-amylase inhibitor reduces the activity of amylase, thereby reducing the amount of starch cracked into glycogen, reducing the intestinal absorption of glycogen, and preventing hyperglycemia after eating. Thereby reducing islet burden, reducing fat synthesis, and reducing weight, especially suitable for obese people and diabetic patients. According to previous reports, the proteins of α-amylase inhibitors are widely found in wheat, rice, sorghum, peanuts, beans and other plants, and α-amylase inhibitors can be extracted by chemical solvents, but chemical ...

AI Technical Summary

Problems solved by technology

[0005] Compared with chemical extraction, supercritical fluid extraction has the advantages of no residue and high efficiency, and is especially suitable for use in the field of food and medicine. The invention patent application with patent number 03815393.9 discloses a method for purifying amylase inhibitors using supercritical carbon dioxide. However, there are two major defects in it. One is that the coarsely ground bean raw material is directly put into supercritical carbon dioxide to remove impurities, and the efficiency is relatively low. Because the raw material kidney bean is a crop with high starch content, impurities such as starch are unnecessary to use A major impurity treated by supercritical carbon dioxide technology, but the patent 03815393.9 also processed impurities such as starch in the supercritical work, resulting in a long extraction time of supercritical carbon dioxide and excessive use of carbon dioxide, making it irrespective of cost and There is a lot of waste in terms of output

Supercritical carbon dioxide equipment is expensive, which makes the cost of single use higher, and at the same time, the amount of single extraction is limited, the extraction efficiency is low, and the cost is enlarged; the second is to repeatedly extract the treated beans twice with heated deionized water, and continue to heat each time 2 hours, then concentrated by heat exchange method and high-temperature spray-drying or freeze-drying to obtain the product, during which high-temperature heating extraction and high-temperature spray-drying are also relatively easy to destroy the activity of amylase inhibitors, and if freeze-drying is used, the efficiency is low and the cost is high