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Abortus Chlamydia psittaci vaccine and preparation method thereof

A kind of chlamydia psittacosis and chlamydia technology are applied in the field of aborted chlamydia psittacosis vaccine and preparation to achieve the effects of single antigen component and low culture cost

Inactive Publication Date: 2009-11-18
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Anti-chlamydia immunization vaccines can effectively prevent and control animal chlamydial diseases, but there is no such vaccine so far, and the market urgently needs new vaccines that are common to all kinds of livestock

Method used

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  • Abortus Chlamydia psittaci vaccine and preparation method thereof
  • Abortus Chlamydia psittaci vaccine and preparation method thereof
  • Abortus Chlamydia psittaci vaccine and preparation method thereof

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Embodiment Construction

[0033] Specific embodiments of the present invention are described below, and the main materials used in the present invention include:

[0034] Strain: Chlamydia psittaci SX5 strain of bovine abortion

[0035] Expression strain: E.coli BL21 (DE3) was purchased from Beijing Biotech Co., Ltd.

[0036] pGEX-4T-1 expression vector: purchased from Beijing Biotech Co., Ltd.

[0037] Chlamydia positive serum: prepared by our laboratory, its titer is above 1:1024.

[0038] Primers: All primers for gene amplification and transformation are designed by the applicant and synthesized by commercial biological companies.

[0039] Enzymes and reagents: RNaseA enzyme, proteinase K, Ex Taq premixed enzyme, HindIII, Sma I, SDS, IPTG, absolute ethanol, DL 2000 DNA Marker, phenol: chloroform: isoamyl alcohol (25:24:1), agarose , DNA recovery kit, etc. were purchased from Treasure Bioengineering (Dalian) Co., Ltd.; pMD18-T cloning vector, E.coli DH5α competent cells, and A-type plasmid small-sca...

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Abstract

The invention relates to a bovine abortus Chlamydia psittaci subunit vaccine and a preparation method thereof. The vaccine is the subunit vaccine formed by Chlamydophila abortus outer membrane protein A (OmpA), and the preparation method of the vaccine comprises the steps of extracting bacterial total DNA to be served as a template from the Chlamydophila abortus, designing two pairs of specific primers and conducting nested polymerase chain reaction and amplification to obtain the complete gene of the Chlamydophila abortus outer membrane protein A (OmpA), and cloning the gene to a prokaryotic expression vector to obtain a recombinant expression vector; using the recombinant expression vector to transform colon bacillus competent cells, extracting recombinant plasmid and screening out positive clone by PCR and enzyme cutting identification; and transferring the recombinant plasmid into the colon bacillus and conducting induction expression to obtain the antigen protein used for the vaccine.

Description

technical field [0001] The invention relates to a vaccine and a preparation method of the vaccine. Specifically, the invention is a subunit vaccine of bovine aborted Chlamydia psittaci. Background technique [0002] Animal chlamydial disease is a chronic infectious disease that exists widely in all parts of the world and is generally endemic or localized. Although the disease does not spread as quickly as a type of infectious disease, the harm it causes in the diseased area is no less than that of Some severe diseases, especially poultry and livestock raised under modern intensive conditions. [0003] As early as the 1920s, the Americans (1923) first reported the cattle endemic abortion syndrome; by 1956, the Germans used serological experiments and cell culture techniques to prove that the former federal German cow epidemic abortion was caused by Caused by chlamydia infection. Later, many countries in Europe, Canada, the former Soviet Union, Japan, and India also reported...

Claims

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Application Information

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IPC IPC(8): A61K39/118A61P31/00A61P15/06G01N33/53C12N15/70C12P21/02C12R1/19
Inventor 邱昌庆宋竹青周继章曹小安郑福英蔺国珍宫晓炜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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