Kit for detection of abortion chlamydia psittaci and method

A technology of Chlamydia psittacosis and a kit, which is applied in the field of kits for detecting microorganisms, can solve the problems of cumbersome operation, unsuitable for clinical and grassroots production and use, insufficient sensitivity and the like, and achieves the effect of shortening the reaction time.

Inactive Publication Date: 2012-11-21
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

With the development of molecular biology, the common PCR method (Zhou Jizhang et al., 2007) has been widely used in the detection of this pathogen because of its advantages of sensitivity, rapidity, and no need for live bacteria operation, but it requires special instruments and is cumbersome to operate, so it is not suitable for clinical practice. an

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  • Kit for detection of abortion chlamydia psittaci and method
  • Kit for detection of abortion chlamydia psittaci and method
  • Kit for detection of abortion chlamydia psittaci and method

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Embodiment Construction

[0018] The present invention is further described below in conjunction with embodiment, but the present invention is not limited to the content of embodiment.

[0019] (1) Preparation of test samples

[0020] C. psittaci vaccine strains D13, A and SX5 of Chlamydia psittaci were isolated from pigs, sheep and cattle with abortion symptoms, and were isolated, identified and preserved by the Key Open Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences ; The plasmid pMD-MOMP containing the MOMP gene of SX5 strain was constructed by our laboratory; the plasmid Cpn0695-pGEX-6p containing the MOMP gene of Chlamydia pneumoniae (C.pneumoniae) was donated by Professor Zhong Guangming, Department of Immunology and Microbiology, University of Texas Health Science Center; Trachoma Chlamydia, Brucella, Escherichia coli, Vibrio cholerae and Clostridium perfringens strains were kept by our laboratory.

[0021] (2) Establish a...

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Abstract

The present invention relates to a kit for detecting microorganisms, particularly a kit for detection of abortion chlamydia psittaci for pigs, sheep and cattle detection, and a preparation method and a use method thereof. The kit for detection of abortion chlamydia psittaci of the present invention comprises two pairs of specific primers composed of an outer primer and an inner primer, wherein the sequence of an upstream outer primer F3 is SEQ ID No. 1; the sequence of a downstream outer primer B3 is SEQ ID No. 2; the sequence of an upstream inner primer FIP is SEQ ID No. 3; and the sequence of an downstream inner primer BIP is SEQ ID No. 4. The kit of the invention has high sensitivity and high specificity, is simple and convenient in the detection method, and can be applicable to detection of pigs, sheep and cattle.

Description

technical field [0001] The invention relates to a kit for detecting microorganisms, in particular to a kit for detecting aborted chlamydia psittaci in pigs, sheep and cattle, as well as its preparation and use methods. Background technique [0002] Chlamydia (Chiamydiaceae) is a class of obligate intracellular parasitic Gram-negative microorganisms between bacteria and viruses, with a size of 0.2-0.4 μm. Chlamydia psittaci (C. psittaci), as a very important species in the genus Chlamydia, is the main pathogenic bacterium of animal chlamydial disease and has a wide range of hosts. Among domestic animals, cattle, sheep, and pigs are more susceptible. It can cause huge economic losses caused by fetal death, greatly reduced milk production, waste of feed and manpower, and has caused great harm to the breeding industry. In addition, aborted Chlamydia psittaci can also infect people and threaten human health. [0003] The study of animal chlamydial disease began at the end of th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 蔺国珍邱昌庆郑福英周继章曹小安宫晓炜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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