Kit for detection of abortion chlamydia psittaci and method

Abstract

The present invention relates to a kit for detecting microorganisms, particularly a kit for detection of abortion chlamydia psittaci for pigs, sheep and cattle detection, and a preparation method and a use method thereof. The kit for detection of abortion chlamydia psittaci of the present invention comprises two pairs of specific primers composed of an outer primer and an inner primer, wherein the sequence of an upstream outer primer F3 is SEQ ID No. 1; the sequence of a downstream outer primer B3 is SEQ ID No. 2; the sequence of an upstream inner primer FIP is SEQ ID No. 3; and the sequence of an downstream inner primer BIP is SEQ ID No. 4. The kit of the invention has high sensitivity and high specificity, is simple and convenient in the detection method, and can be applicable to detection of pigs, sheep and cattle.

Description

technical field [0001] The invention relates to a kit for detecting microorganisms, in particular to a kit for detecting aborted chlamydia psittaci in pigs, sheep and cattle, as well as its preparation and use methods. Background technique [0002] Chlamydia (Chiamydiaceae) is a class of obligate intracellular parasitic Gram-negative microorganisms between bacteria and viruses, with a size of 0.2-0.4 μm. Chlamydia psittaci (C. psittaci), as a very important species in the genus Chlamydia, is the main pathogenic bacterium of animal chlamydial disease and has a wide range of hosts. Among domestic animals, cattle, sheep, and pigs are more susceptible. It can cause huge economic losses caused by fetal death, greatly reduced milk production, waste of feed and manpower, and has caused great harm to the breeding industry. In addition, aborted Chlamydia psittaci can also infect people and threaten human health. [0003] The study of animal chlamydial disease began at the end of th...

AI Technical Summary

Problems solved by technology

With the development of molecular biology, the common PCR method (Zhou Jizhang et al., 2007) has been widely used in the detection of this pathogen because of its advantages of sensitivity, rapidity, and no need for live bacteria operation, but it requires special instruments and is cumbersome to operate, so it is not suitable for clinical practice. and grass-roots production and use

Although the nucleic acid sequence-dependent amplification technique (NASBA) is simpler, faster, and more specific, and has been used in the detection of various viruses, there are problems such as insufficient sensitivity and false positives in the detection of some pathogens by using NASBA (Gao Lightning et al. , 2009)

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