The present invention belongs to field of
biology and
medicine, and especially relates to a novel antibiotic comprising a mimetic
antibody, its preparation methods and uses thereof. The novel antibiotic comprising comprising an
antibody mimetic and a
colicin,or comprising an
antibody mimetic and a channel-forming domain of a
colicin, the
antibody mimetic covalently bonded to the carboxyl end of the polypeptide of the
colicin or the channel-forming domain of the colicin, wherein the colicin is selected from a group consisting of colicin E1, Ia, Ib, A, B, N; wherein said
antibody mimetic being yielded by fusing two complementarity determining regions (CDRs), V H CDR 1 ) and V L CDR through a cognate
framework region (V H FR 2 ) of an immunoglobulin; wherein the immunoglobulin specifically recognizes bacterium porins. Its antibacterial ability is a thousandfold powerful than normal
antibiotics. Due to its unique action mechanism,
drug resistance resulted in
mutation can hardly be acquired by
pathogenic bacteria. And the antibiotic will not hurt normal human cells when it kills
pathogenic bacteria. Therefore, it can be used for manufacturing antibacterial medicament of killing
Diplococcus intracellularis,
vancomycin-resistant enterococci, Methicillin-resistant
Staphylococcus aureus, or
multidrug resistant Pseudomonas aeruginosa.