20 results about "Dendritic spine" patented technology

The invention relates to the technical field of brain science image processing, and discloses a brain tissue neuronal morphology three-dimensional reconstruction and connection group analysis method and system, and the method comprises the steps: carrying out the feature point extraction and matching and optical flow registration of continuous ultrathin section electron microscope images, and obtaining three-dimensional image volume data; carrying out semantic segmentation on cell bodies, dendrites, axons and dendritic spines through a three-dimensional deep convolutional neural network, and driving secondary registration through confidence feedback; detecting synapses and classifying the synapses as excitatory or inhibitory types; executing topology perception skeletonized reconstruction and performing segmentation correction through topology anomaly feedback constraint; and constructing a synaptic connection matrix and executing graph theory analysis.

The invention relates to a tracer compound for whole-cell labelling, comprising: a non-streptavidin- binding biotin analogue, at least one connecting moiety comprising a functional group configured for a click-chemistry reaction, and at least one cross-linkable moiety, preferably wherein the cross-linkable moiety is a (para)formaldehyde cross-linkable group, and the non-streptavidin-binding biotin analogue enables the tracer compound to diffuse within a biological cell with essentially the same properties as biocytin and / or neurobiotin, preferably enabling the detection of cellular protrusions, such as dendrites, dendritic spines and axons. The invention further relates to methods for constructing such tracer compound and for labelling a cell therewith. The invention further relates to kits for carrying out the method according to the invention.

The present disclosure relates to the use of compounds of formula (I), compounds of formula (II), and related analogs for enhancing neurogenesis, enhancing neurite outgrowth, enhancing nerve growth, nerve connectivity, synaptic density, dendritic spine density, dendritic spine size, and excitatory neurotransmission in the brain (e.g., in the medial prefrontal cortex), and to methods of enhancing neurogenesis, enhancing neurite outgrowth, enhancing nerve growth, nerve connectivity, synaptic density, dendritic spine density, dendritic spine size, and excitatory neurotransmission in the brain (e.g., in the medial prefrontal cortex). And other methods and uses related thereto.

The invention discloses a method for observing the shape and number of synapses in a dentate gyrus region based on image analysis, and relates to the technical field of neurobiology. The method comprises the following steps: S1, tissue treatment: after an experimental animal is anesthetized, pouring a buffer solution and a stationary solution into the heart in stages, separating hippocampus tissue, trimming and fixing, pre-cooling, slicing and preserving at low temperature; s2, dyeing and marking: performing targeted marking on the dentate gyrus area of the sea horse by adopting DiI particles through a fine tungsten steel through needle, and preserving at low temperature after incubation; s3, fixing and mounting: fixing for the second time, and mounting with an anti-fluorescence quenching agent after DAPI nuclear dyeing; s4, confocal imaging is carried out, exclusive parameter layer scanning is set, and qualified dendritic spine images are screened; and image quantitative analysis can be further carried out, and accurate quantification is realized according to classification standards. The method is standard in operation, greatly improves sample quality and experimental result credibility, optimizes experimental efficiency and controllability and reduces technical threshold by means of targeted marking and standardized processes, and is suitable for relevant basis and application research of the hippocampal synapses.

Changes in Reelin levels as well as Reelin signaling alter cognitive function. This can be accomplished by administering a therapeutically effective amount of a repeat fragment of Reelin, or a construct formed from fragment repeats of Reelin to a patient or subject. Changes to Reelin levels can be used to treat various neurodegenerative diseases, neuronal insults, or stroke, such as fragile X syndrome, William's syndrome, Rett syndrome, Down's syndrome, Angelman syndrome, autism, ischemia, hypoxia, Alzheimer's disease, and schizophrenia: Reelin can also be used to alter dendritic spine density, diminished long-term potentiation, and diminished synaptic plasticity and associative learning deficits. Constructs formed from repeat region 3 of full length Reelin and repeat region 5 of full length Reeling or repeat region 3 of full length Reelin and repeat region 6 of full length Reelin have been found particularly useful.

The application relates to the technical field of brain science image processing, and discloses a brain tissue neuron morphology three-dimensional reconstruction and connection group analysis method and system. The method comprises the following steps: feature point extraction and matching and optical flow registration are performed on continuous ultrathin section electron microscope images to obtain three-dimensional image body data; a three-dimensional deep convolutional neural network is used for semantic segmentation of cell bodies, dendrites, axons and dendritic spines, and secondary registration is driven through confidence feedback; synapses are detected and classified into excitatory or inhibitory types; topological perception skeletonization reconstruction is performed, and segmentation correction is constrained through topological anomaly feedback; and a synapse connection matrix is constructed, and graph theory analysis is performed.

The invention discloses application of Trolox in preparation of a medicine for treating neurodevelopment disorder related diseases, and belongs to the technical field of biological medicine. The invention firstly discovers that Trolox has new application of drugs for preventing and / or treating neurodevelopment disorder related diseases; furthermore, the Trolox restores the neural connectivity by repairing the densities of the damaged neuron dendritic spines of the brain hippocampus and / or striatum; abnormal dopaminergic signal channels are corrected, so that expression of key receptors and downstream molecules thereof is recovered to be normal, and therefore, diseases related to neurodevelopment disorders are prevented and / or treated; the neuroprotective effect of Trolox depends on the activation of dopamine D1 and D2 receptor pathways; in addition, the invention discloses the potential of Trolox in the aspect of targeted repair of the core pathology link of the neurodevelopmental disorder for the first time, and a clear candidate compound and an action target are provided for developing a novel medicine for treating the diseases.

The application belongs to the technical field of biological medicine, and particularly relates to application of quinacrine, a pharmaceutically acceptable salt or a solvate thereof in preparation of a medicine for treating hepatic encephalopathy. The application finds that quinacrine can target and inhibit a PLA2G3 / cGAS-STING signal axis of microglia cells, and is used for treating hepatic encephalopathy. Specifically, quinacrine blocks a decrease of mitochondrial membrane phospholipid PE and activation of a cGAS-STING pathway induced by high ammonia by inhibiting PLA2G3 activity, thereby reducing release of proinflammatory factors (IL-1beta, IL-6, TNF-alpha, etc.), reversing abnormal activation of microglia cells to proinflammatory type, and inhibiting synaptic damage of neurons (restoring expression of PSD95 and SYN and morphology of dendritic spines), and finally improving cognitive function.

The application discloses application of a rabies virus mutant in traditional two-photon microscope deep brain cortex neuron imaging, and the rabies virus mutant is CVS-N2c-ΔG-GCaM6s. The application also discloses a calcium imaging virus containing the rabies virus mutant. The rabies virus mutant in the application is used as a retrograde labeling virus, has the advantages of high brightness of fluorescent expression of the labeled neuron, and has fast expression speed, thereby greatly saving time cost. In addition, the rabies virus mutant has the ability of labeling the complete structure of a cell, can completely record the functional signal from a dendritic spine to a cell body, provides a suitable method for studying input-output integration of single neuron information, especially deep neuron. The rabies virus mutant can realize high-quality functional imaging of deep neuron under the conventional two-photon microscope, and has the technical characteristics of high safety and low use cost.

The application relates to the technical field of image recognition and discloses a dendritic spine continuous learning mapping method and device for two-dimensional two-photon calcium imaging, which comprises the following steps: acquiring a two-photon calcium imaging dendritic spine image data set, pre-processing the two-photon calcium imaging dendritic spine image data set to obtain a first image data set, randomly dividing the first image data set to obtain k second image data sets, generating pseudo labels for second image data in the first second image data set under single-point prompting by a basic segmentation model to obtain a first second label image data set, training the first second label image data set to obtain the basic segmentation model, predicting the jth second image data set by the basic segmentation model, correcting the jth second image data set to form a jth second label image data set, integrating the second labels of all rounds into incremental training to update the segmentation model, and obtaining a continuous learning model; and processing the first image data set on a multi-scale feature map by using the model to obtain a dendritic spine instance mask set.

The invention relates to the technical field of image recognition, and discloses a two-dimensional two-photon calcium imaging dendritic spine continuous learning mapping method and device, and the method comprises the steps: obtaining a two-photon calcium imaging dendritic spine image data set, carrying out the preprocessing of the two-photon calcium imaging dendritic spine image data set, obtaining a first image data set, carrying out the random division of the first data set, and obtaining k second image data sets; generating a pseudo label for second image data in the first second image data set through the basic segmentation large model under single-point prompt to obtain a first second label image data set, and training according to the first second label image data set to obtain a basic segmentation model; the jth second image data set is predicted through the basic segmentation model, a jth second label image data set is formed through correction, all rounds of second labels are merged into incremental training to update the segmentation model, and a continuous learning model is obtained; and processing the first image data set on a multi-scale feature map by using the model to obtain a dendritic spine instance mask set.

The disclosure provides a dendritic spine annotation method and tracking method of a neuron fluorescence microscopic image, which can be applied to the technical field of image processing and the technical field of neurobiology. The annotation method comprises: determining a neuron region in the neuron fluorescence microscopic image according to a pixel value of a pixel point in the neuron fluorescence microscopic image; determining a candidate peak point located in the neuron region from the pixel point based on a local peak value of the pixel value, wherein the distance between adjacent local peak values is greater than a preset threshold; determining a target peak point matched with the dendritic spine in the neuron fluorescence microscopic image according to the pixel value change information of the candidate peak point; and annotating a dendritic spine region in the neuron fluorescence microscopic image based on the target peak point and using gradient change information of the pixel points in a preset search box to obtain a target annotation image.

Provided herein are tetracyclic heterocyclic compounds which can be useful for methods of treating a disease or for increasing neural plasticity. The compounds can also be useful for increasing dendritic spine density.

Provided herein are tetracyclic heterocyclic compounds useful in methods of treating a disease or for increasing neural plasticity. These compounds can also be used to increase dendritic spine density.

The invention discloses application of a maravil and CCL5 neutralizing antibody to treatment of sepsis-related encephalopathy, and belongs to the field of medicinal chemistry. In-vivo experiments prove the novel application of the CCR5 antagonist malaviif and the CCL5 neutralizing antibody in treatment of sepsis-related encephalopathy (SAE), the survival rate of sepsis mice can be remarkably increased to 60% or above from about 30%, and anxiety-like behaviors and learning and memory dysfunctions of the mice can be effectively improved; according to the core mechanism, by inhibiting CCL5-CCR5 signal channels, pathological damage of hippocampal neurons is relieved, the level of key neuroinflammatory factors (IL-6, IL-1beta, CD68 and HMBG-1) is reduced, and by protecting dendritic spine density and synaptic related protein expression, abnormal synaptic phagocytosis of microglia is inhibited, so that comprehensive protection on the structures and functions of nerve synapses is achieved, and the application prospect is wide. And a verified brand-new target spot and an effective strategy are provided for clinical SAE treatment.

The invention discloses application of lactobacillus rhamnosus to preparation of a product for treating hippocampus injury caused by chronic fatigue. The invention discloses that the lactobacillus rhamnosus BD4047 has good neuron and synaptic protection capability for the first time, and can improve hippocampus neuron damage caused by chronic fatigue, increase the density of hippocampus neuron dendritic spines, increase the length of the dendritic spines and increase the number of branch intersection points of the dendritic spines; the synaptic injury caused by chronic fatigue is improved, the synaptic gap width is reduced, the thickness of a synaptic compact region is increased, the length of a synaptic active region is prolonged, the increase of the number of synaptic vesicles and the increase of the number of SYP spots are promoted, and a remarkable synaptic repair effect is achieved; in addition, mouse hippocampus microglial cell activation caused by chronic fatigue can be inhibited, hippocampus injury caused by chronic fatigue and diseases related to the hippocampus injury can be comprehensively improved, and therefore the composition has a wide prospect.

The invention discloses an application of fluconazole in preparation of a medicine for treating autism spectrum disorder. Animal experiment research shows that fluconazole can improve the social disorder performance of an SHANK3 KO mouse, the number of dendritic small ridges of the prefrontal cortex of the SHANK3 KO mouse can be remarkably increased, the level of synaptic scaffold protein in the prefrontal cortex of the SHANK3 KO mouse can be remarkably improved, and the fluconazole has repairing and remodeling effects on synapses. Cell experiment research shows that fluconazole can reverse neurotoxicity caused by glutamic acid, so that cell activity is recovered to a relatively high level, and the fluconazole has concentration dependence; glutamic acid can enable nerve cells to lose normal cellular morphology, tree mutation is short, cell bodies become large, fluconazole can reverse the phenomenon, and the cellular morphology of neurons is recovered. Therefore, fluconazole shows a relieving effect on the core symptom of ASD, and has application potential and prospect as an ASD treatment drug.

The invention relates to the technical field of optical imaging, in particular to a microscope objective lens system and method with a large object space view and a long working distance. Comprising a microscope objective lens system, the microscope objective lens system comprises twenty optical lenses arranged on the same optical axis, two solution layers and a cover glass, and a relay group and an objective lens are sequentially arranged from the object side to the image side. Combination of a large object space visual field (the diameter ranges from 7.5 mm to 8.5 mm), a long working distance (7.5 mm to 8.5 mm), a high collection numerical aperture (0.9-1.1) and a numerical aperture (0.5-0.6) is achieved for the first time, the technical contradictions that a traditional objective lens has long working distance sacrifice resolution and the large visual field is difficult to consider high collection efficiency at the same time are solved, and the fine structure analysis requirement of the single neuron and dendritic spine level is met.

In some embodiments, a method of promoting dendritic spine generation in a patient is provided, the method comprising administering to a patient in need thereof a therapeutically effective amount of a compound that binds to fascin at least at binding site 2 or binding site 3.

This invention discloses the application of the Siegesbeckia orientalis composition in the preparation of drugs to improve cognitive impairment after heart failure (CHF). The composition consists of Astragalus membranaceus extract, Siegesbeckia orientalis extract, and Periploca sepium extract. Experiments have shown that the Siegesbeckia orientalis composition can significantly improve spatial memory and working memory in CHF rats, and improve cognitive function; it can significantly inhibit the release of tumor necrosis factor α and interleukin 1β in the hippocampus of CHF rats, and increase the release of interleukin 10 and brain-derived neurotrophic factor; it can significantly reduce the loss and damage of Nissl bodies in the brain tissue of CHF rats; and it can significantly improve the ultrastructure of neurons in the hippocampus of CHF rats, regulate synaptic plasticity, and promote the growth of dendrites and dendritic spines.