45 results about "Dendritic spine" patented technology

A method for visualizing an active synapse wherein said method comprises: (a) exposing cells forming the active synapse to a biomarker comprising at least fragment C of tetanus toxin and a reporter protein; and (b) visualizing the biomarker; wherein the accumulation of the biomarker into dendritic spines of the cells allows visualization of an active synapse. Also, a method for screening molecules capable of modulating synapse activity is provided. A kit useful for the early diagnosis of neurodegenerative disease comprises a biomarker comprising at least fragment C of tetanus toxin and a reporter protein.

A method for visualizing an active synapse wherein said method comprises: (a) exposing cells forming the active synapse to a biomarker comprising at least fragment C of tetanus toxin and a reporter protein; and (b) visualizing the biomarker; wherein the accumulation of the biomarker into dendritic spines of the cells allows visualization of an active synapse. Also, a method for screening molecules capable of modulating synapse activity is provided. A kit useful for the early diagnosis of neurodegenerative disease comprises a biomarker comprising at least fragment C of tetanus toxin and a reporter protein.

The invention discloses a nerve dendritic spine image classification method based on multiresolution fractal features. The method includes the following steps of (1) extracting features of nerve dendritic spine images to obtain the multiresolution fractal features of the nerve dendritic spine images; and (2) classifying the nerve dendritic spine images based on the multiresolution fractal features of the nerve dendritic spine images in the method of linear discriminant analysis (LDA). According to the method, classification precision is high and classification results are stable.

The invention discloses a femtosecond pulse laser modulator and a fast high resolution miniature two-photon microscope having the same. The femtosecond pulse laser modulator comprises a negative dispersion optical path and a positive dispersion optical path, wherein the negative dispersion optical path comprises a laser input optical fiber for transmitting a pulse-widened prechirp compensated laser beam to a scanning imaging portion; and the positive dispersion optical path is located between a femtosecond pulse laser device and the negative dispersion optical path and is used for compensating the negative dispersion caused by the laser input optical fiber in a laser transmission process. The femtosecond pulse laser modulator is capable of providing a favorable condition for the fast high resolution miniature two-photon microscope to stably observe the dendrites and the dendritic spine of unrestrained animals in a natural physiological environment.

Provided herein are pyrido[2,3-D]pyrimidin-7(8H)-one compounds and compositions useful as PAK inhibitors. Also provided herein are methods of utilizing these compounds for the treatment of neuropsychiatric conditions. The compounds modulate dendritic spine morphology and / or synaptic function.

The invention provides a wintergreen pure polysaccharide LTC-0-1 and an application thereof, which belong to the field of medicines and healthcare foods. The invention discloses an extracting method of the wintergreen pure polysaccharide LTC-0-1, a composition of the LTC-0-1 and a molecular weight of the LTC-0-1; it is proved by experiments that the LTC-0-1 can be used for increasing average lengths of hippocampal neurons of a GFP green fluorescent protein transgenic mouse, increasing the number of the neurons, obviously increasing dendritic spine densities of neuron dendrons in a unit length, increasing neuronal post-synaptic membrane protein expression and promoting the neurons to be more mature; the LTC-0-1 can be also used for inducing neuron-like differentiation of PC12 cells, promoting enation of the cells, enhancing activity of cell acetylcholinesterase AchE and increasing expression index of cell growth related protein GAP-43 and obtains neurotrophic activity; and the LTC-0-1 shows a growth promoting effect, a differentiation effect, a maturing effect and a damaged nerve cell function recovery effect on nerve cells and has broad prospects in development and application in the technical field of medicines and healthcare foods.

The invention belongs to the technical field of pharmacy, and particularly relates to an application of danshinolic acid A in preparation of medicines for preventing and treating AIDS encephalopathy as well as nerve damages caused by an HIV-1Tat protein. According to the invention, an HIV-1Tat protein damage type nerve cell line PC 12 and nerve cells on mouse brain slices are protected by using danshinolic acid A with different concentrations, and the effect of reducing the death of the nerve cells caused by the Tat protein of the danshinolic acid A is respectively detected by an MTT experiment, LDH detection and a Tunnel experiment; and dendritic spines are labeled by scattering Dil gold particles, and the effect of reducing Tat protein damage synaptic connectivity of the danshinolic acid A is detected. Through statistical analysis of experimental results, the danshinolic acid A is found to have a good protection effect against damages of the nerve cells caused by the Tat protein and can be used for preparing the medicines for preventing and treating the nerve damages caused by the Tat protein as well as the AIDS encephalopathy.

The invention relates to a calculation method of neural synaptic plasticity based on calcium concentration, which relates to the field of brain simulation, in particular to the calculation problem ofneural synaptic plasticity of impulse neural network in brain simulation. The first is the calculation of calcium ion concentration. According to the membrane potential values of presynaptic neurons and postsynaptic neurons at the initial time t0 and the initial connecting weight w0 of synapses, the calcium ion concentrations in dendrites and spines at the next time t1 are calculated respectivelyfor the synapses that need to be calculated. Secondly, according to the calcium ion concentration in dendritic spine, the direction of weight change was obtained by comparing with the calcium ion concentration threshold Ca0s and Ca1s. According to the synaptic state tag Tag and the plasticity related protein PRP concentration, the change of synaptic weight was calculated, and the new weight at time t1 was obtained. the above procedure is repeated to calculate the strength of synaptic connections within the simulation time. The method of the invention is applied to constructing a brain-like neural network, completing the simulation of the learning and memory process required by the brain-like intelligence, realizing the universal strong artificial intelligence, and is applied to intelligentmedia, medical treatment and the like.

The invention discloses application of a GCS inhibitor in preparation of a medicine for treating cocaine addiction, and belongs to the field of drug rehabilitation medicines. Experiments prove that the GCS inhibitor can specifically reduce the content of glucosylceramide sphingolipid in the nucleus-isolated brain region, reduce the dendritic branch number and the dendritic spine density, and effectively inhibit the behavioral effects (behavioral sensitization, reward effect and autonomous administration behavioral effect) of cocaine on mice. The GCS inhibitor is used for preparing the medicine for treating cocaine addiction, and has a good application prospect.

The present invention provides methods of slowing or reversing the loss of memory and learning comprising the steps of contacting an effective amount of a PKC activator with a protein kinase C (PKC) in a subject identified with memory loss slowing or reversing memory loss. The present invention provides methods of stimulating cellular growth, neuronal growth, dendritic growth, dendritic spine formation, dendritic spine density, and the translocation of ELAV to proximal dendrites, and synaptic remodeling. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to stimulate the synthesis of proteins sufficient to consolidate long-term memory. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to downregulate PKC.

The invention discloses a nerve dendritic spine image classification method based on multiresolution fractal features. The method includes the following steps of (1) extracting features of nerve dendritic spine images to obtain the multiresolution fractal features of the nerve dendritic spine images; and (2) classifying the nerve dendritic spine images based on the multiresolution fractal features of the nerve dendritic spine images in the method of linear discriminant analysis (LDA). According to the method, classification precision is high and classification results are stable.

The present invention provides methods of slowing or reversing the loss of memory and learning comprising the steps of contacting an effective amount of a PKC activator with a protein kinase C (PKC) in a subject identified with memory loss slowing or reversing memory loss. The present invention provides methods of stimulating cellular growth, neuronal growth, dendritic growth, dendritic spine formation, dendritic spine density, and the translocation of ELAV to proximal dendrites, and synaptic remodeling. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to stimulate the synthesis of proteins sufficient to consolidate long-term memory. The present invention also provides methods of contacting a protein kinase C (PKC) activator with a PKC activator in a manner sufficient to downregulate PKC.

The invention provides a method for establishing a neuron dendritic spine development mode based on a reaction diffusion model, which comprises the steps of formulating a dendritic spine shape classification standard by using the neuron dendritic spine shape of a rat brain hippocampus, simulating the growth process of a single dendritic spine under different conditions by using the reaction diffusion model, classifying simulation results by using a shape classification standard, and further simulating a process of growing dendritic spines with different densities by using the reaction diffusion model under different conditions so as to obtain neuron dendritic spines with different modes. Compared with an existing research method, a large number of animal experiments are effectively avoided; and meanwhile, the reaction diffusion model is applied to the establishment process of the neuron dendritic spine development mode for the first time, and a foundation is laid for subsequent research on the pathogenesis of diseases caused by abnormal dendritic spine modes.