Application of danshinolic acid A in preparation of medicines for preventing and treating AIDS encephalopathy

A technology of AIDS encephalopathy and salvianolic acid, applied in the application field of drugs for the prevention and treatment of nerve damage caused by HIV-1 Tat protein

Inactive Publication Date: 2014-06-18
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no related reports about the protection of salvia miltiorrhiza or salvianolic acid A on nerve damage caused by HIV-1 Tat protein or its application in HAND treatment.

Method used

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  • Application of danshinolic acid A in preparation of medicines for preventing and treating AIDS encephalopathy
  • Application of danshinolic acid A in preparation of medicines for preventing and treating AIDS encephalopathy
  • Application of danshinolic acid A in preparation of medicines for preventing and treating AIDS encephalopathy

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Embodiment 1

[0027] (1) Main reagents, cells and instruments

[0028] HIV-1Tat protein was purchased from Shenzhen Baiwansen Bio-Trade Co., Ltd. (Prospec, USA, batch number: 212PCLADEB28). Salvianolic acid A (batch number: 96574-01-5) and tanshinone IIA (batch number: 568-72-9) were purchased from Zhengzhou Fengyao Agriculture Co., Ltd. Rat adrenal pheochromoma cell line (PC12) was purchased from Shanghai Institute of Cells, Chinese Academy of Sciences. MTT was purchased from Sigma (batch number: MTBF7873V); LDH (lactate dehydrogenase) detection kit was purchased from Nanjing Jiancheng Institute of Bioengineering; Tunel detection kit was purchased from Promega, USA (batch number: 0000073091). Inactivated fetal bovine serum (batch number: 111023) was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; horse serum (batch number: 841843), DMEM medium (batch number: 823186) and MEM medium (batch number: 793870) were purchased from From Gibco; other reagents were of analytica...

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Abstract

The invention belongs to the technical field of pharmacy, and particularly relates to an application of danshinolic acid A in preparation of medicines for preventing and treating AIDS encephalopathy as well as nerve damages caused by an HIV-1Tat protein. According to the invention, an HIV-1Tat protein damage type nerve cell line PC 12 and nerve cells on mouse brain slices are protected by using danshinolic acid A with different concentrations, and the effect of reducing the death of the nerve cells caused by the Tat protein of the danshinolic acid A is respectively detected by an MTT experiment, LDH detection and a Tunnel experiment; and dendritic spines are labeled by scattering Dil gold particles, and the effect of reducing Tat protein damage synaptic connectivity of the danshinolic acid A is detected. Through statistical analysis of experimental results, the danshinolic acid A is found to have a good protection effect against damages of the nerve cells caused by the Tat protein and can be used for preparing the medicines for preventing and treating the nerve damages caused by the Tat protein as well as the AIDS encephalopathy.

Description

technical field [0001] The invention belongs to the field of pharmaceutical technology, and relates to a new application of salvianolic acid A, a water-soluble component of traditional Chinese medicine Salvia miltiorrhiza Application of protein-induced nerve injury drugs. Background technique [0002] AIDS encephalopathy, now also known as HIV-associated neurocognitive disorders (HAND, HIV-associated neurocognitive disorders), is characterized by motor, behavioral and cognitive impairments, and has recently been divided into three forms according to the severity of the disease: asymptomatic neurocognitive disorders Cognitive impairment (Asymptomatic neurocognitive impairment, ANI), mild neurocognitive disorder (mild neurocognitive disorder, MND) and HIV-associated dementia (HIV associated dementia, HAD). [0003] The detailed mechanism of HIV-1 causing nerve damage remains to be further studied, but it is certain that the neurotoxic protein of HIV-1 damages neurons directly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P31/18A61P25/00
Inventor 刘红亮王靖凯史朝晖胡磊刘彬邓锦波徐括喜
Owner HENAN UNIVERSITY
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