44results about How to "Avoid experimentation" patented technology

The invention discloses a method with a strong discrimination power, which is used for calculating and recognizing active ingredient of a natural product on the basis of compound characteristics. The method is characterized in that whether a molecule has biological activity or not can be calculated and recognized in an extractive molecule of the natural product with a known structure by utilizing descriptors of compound characteristics. The method comprises the following steps: step A, a training set and a predicting set of natural product extractive molecules used for model building are structured; step B, molecular structure files of the training set and the predicting set are collected; step C, the descriptors of the compound characteristics can be figured out by utilizing the molecular structure files; step D, classification modeling can be performed on the training set by using a machine learning software according to the descriptors of the compound characteristics; and step E, the molecule of the predicting set has biological activity or not can be identified by the machine learning software with the combination of step D. The method of the invention has a good application prospect in high flux virtual screening and the study of synergistic effect of natural products.

The invention provides an artificial slope surface structure for physical resistance experiment measurement,which comprises a base,an angle adjusting disc and a sliding block; an upward side guard board is fixedly connected with the front end and the rear end of the base respectively,a charging head is arranged in the front end plane of the side guard board at the front end of the base,and a datatransmission interface is arranged in the rear end plane of the side guard board at the rear end of the base. According to the invention,an inclined plate capable of ignoring force of friction,a first-stage friction bottom plate,a second-stage friction bottom plate and a third-stage friction bottom plate are arranged,and the friction resistance relationship among the first-stage friction bottom plate,the second-stage friction bottom plate and the third-stage friction bottom plate is 1-fold,2-fold and 3-fold sequentially,thereby being capable of forming a sharp contrast experiment at the time of performing the physical experiment,and facilitating accepting more vivid learning experience for students,regular growth of friction resistance facilitates summarizing various stress relationships on the inclined plate for the students.

The invention discloses a novel stimulating electrode based in-vitro brain slice electrical signal recording device which comprises a brain slice recording slot, a brain slice storage slot, a gas supply device, a perfusing drug-delivery device, a temperature control device, an electrode fixing and operating device, a neural network signal recording device and a dissecting microscope. Two microelectrodes are fixed to the electrode fixing and operating device and are inserted into a feeding groove from a top cover of the brain slice recording slot, the other end of each microelectrode is connected with the neural network signal recording device through a lead, the two microelectrodes are a stimulating microelectrode and a recording microelectrode respectively, and the stimulating microelectrode is an epoxy resin/graphene/nano-copper composite material electrode. The novel stimulating electrode based in-vitro brain slice electrical signal recording device effectively overcomes the defect that an old in-vitro brain slice recording device cannot automatically control the temperature, is provided with a smaller feeding groove, is inconvenient to use and makes records inaccurate and does not efficiently stimulate microelectrodes due to shake caused by various situations.

The invention provides a regulation and control method for producing xylose by transforming xylan by use of escherichia coli and belongs to the field of biochemical engineering. According to the regulation and control method, the constitutive promoters gapA, pgi, pykA and pykE of the escherichia coli are cloned and characterized, and applied to constructing an approach of producing the xylose by transforming the xylan; and the expression of OsmY-Xyn (the fusion protein of carrier protein and xylanase) and the expression of OsmY-Xyl (the fusion protein of carrier protein and xylosidase) are controlled, respectively, and the transcription of the two fusion proteins is terminated by use of terminators rrnb and T7, respectively. The approach is imported into the escherichia coli BL21 (DE3) in the form of plasmids to obtain a genetic engineering strain BL21-Xyn-XYl capable of producing the xylose from the xylan. The method of regulating and controlling the approach of producing the xylose by transforming the xylan by use of the escherichia coli by adopting the characterized constitutive promoters is adopted, the addition of an inducer and the optimization of induction conditions are avoided, and then the production process can be simplified and the production cost can be reduced.

The invention relates to a method for determining the etching time of a plow groove, which comprises the steps as follows: a first mask which has a plow groove design is provided and the light transmission ratio of the first mask is obtained; the value of the light transmission ratio is input into the functional relationship of the light transmission ratio of a second mask which is obtained beforehand, and the etching time of the plow groove to calculate the etching time of the plow groove which is corresponding to the plow groove design of the first mask, wherein, the second mask is provided with the plow groove design and the plow groove design of the second mask and the plow groove design of the first mask have the same technology nodes. The invention also provides a manufacturing method for the isolation of a shallow plow groove. The technique for obtaining the etching time of the plow groove of the invention is relatively simple and can save time.

The invention discloses a modeling approach of hydraulic mount, and belongs to the automotive technology field. The modeling approach comprises: based on the assumed condition establishing physical model of hydraulic mount, and the hydraulic mount is composed of a rubbing spring, an upward fluid chamber, a downward fluid chamber and an inertia track. The assumed condition is composed of neglecting the quality of rubbing spring, hypothesizing the fluid pressure acting inside the inertia track is equal; according to the physical model, establishing finite element model; based on the finite element model, the technology is capable of gaining model parameter and stimulation model through directly searching for excellent methods to identify system. The modeling approach has the advantages of being easier to improve the precision of the stimulation model.

The invention relates to an activity detection method of ester type catechin synthetase. The method comprises five steps, namely preparation of enzyme solution, preparation of reaction solution, preparation of contrast solution, detection of enzyme activity, and calculation of the enzyme activity. The ester type catechin serving as an enzyme reaction product is detected by using high performance liquid chromatography so as to detect the enzyme activity, and interference of oxidase and ester type catechin hydrolase on enzyme activity detection can be avoided by adding antioxidant and hydrolaseinhibitor in the reaction system can be avoided. The method is simple, has strong accuracy and high sensitivity, and can accurately detect the reaction product of the ester type catechin synthetase; and the operation is convenient, the detection time is short, fussy experiment operation is avoided, and the detection can be finished in 2 to 3 hours.

The invention provides an automobile fuel pump. According to the automobile fuel pump, by means of a rotary turbine, the automobile fuel pump comprises pumping fuel into a housing of the pump and pressurizing the fuel, and discharging the pressurized fuel out of the housing of the pump; 37 blade grooves are formed in the periphery of the turbine, and the blade grooves are of rounding and fine-tuning on base of a sinusoidal distribution rule, in line with a mathematical formula; a non-equally spaced blade distribution way is selected in a targeted mode, the pressure pulsation of the turbine with non-equally spaced blades can be perfectly reduced without a large number of experiments or analog computation, and the static balance of the turbine is perfectly guaranteed.

The invention discloses a method and a device for testing the amount of degassed gas lost in a shale gas well, comprising a coring barrel and a coring drill bit (11), and the coring barrel has an outer wall (1) of the coring barrel and an inner wall (5) of the coring barrel ), a rubber cylinder (10), a partition (12), a liquid level sensor (9), a high-pressure micro-constant flow pump (6), and a control unit are sequentially installed in the inner wall (5) of the upper part of the coring drill bit (11) (4), battery (3), coring and pitching (2), high-pressure micro-constant flow pump (6) is installed in the inner wall (5) of the coring cylinder by a support (7), and the support (7) is provided with a pressure sensor ( 16), the high-pressure micro-constant-flow pump (6) is connected with the electromagnetic valve (15) through the liquid discharge pipeline (13), and the other end of the high-pressure micro-constant-current pump (6) is provided with a suction port (8), and the rock core barrel dividing plate (12 ) and the inner wall (5) of the coring barrel to form a test cavity (14); one side of the coring and throwing ball (2) is provided with a micro switch (17). The invention solves the problem of gas loss in shale gas content testing, and the testing method is direct, simple and reliable, and the testing cost is low.

The invention discloses a three-dimensional cell scaffold printing method based on cell state feedback. According to the invention, a marker is added during cell sap culture, so that the purpose of real-time tracking of cell distribution is realized; in a process of manufacturing a support material, test solutions for detecting cell secretion are mixed, so that the purpose of real-time detection of a differentiation process of stem cells is realized. Through the cell tracking and the real-time detection of the cell differentiation process, real-time monitoring of sedimentation of gelatin carrying cells is realized, so that the study of a direct relation between cell survival and differentiation and all parameters (such as deposition velocity and piezoelectric pulse width) of the sedimentation technology is facilitated, thereby greatly shortening the study period and reducing the cost.

The invention provides a vehicle-mounted air conditioner duct design method based on CAE emulation technique. The method comprises a modeling procedure, an emulation analysis procedure, an air distribution and judgment procedure and an optimization procedure, wherein the optimization procedure means that one or more of multiple air duct optimization ways are adopted selectively to conduct optimization to an air duct three-dimensional model, the multiple air duct optimization ways comprise the steps of changing bending angles at one or more positions of one or more divergent air ducts; changing the cross sections at the one or more positions of the one or more divergent air ducts; arranging stream guidance structures used for reducing flow or draining flow at the upstream of air duct forks of the one or more divergent air ducts. By means of the design method, the problems that in the prior art, the cycle is long and the cost is high in the classified optimization of vehicle-mounted air conditioner ducts are effectively solved.

The invention discloses a safe and efficient TCR gene targeted to CD19. The TCR gene is characterized in that a nucleotide sequence of the TCR gene is SEQ ID NO.1, and the TCR gene is formed by CD8leader, CD19scFv, TRBC, T2A, CD8leader, myc-tag and TRAC which are sequentially connected in series. The invention also discloses a preparation method of the TCR gene, a plasmid with the TCR gene, a kitand an application. The TCR gene disclosed by the invention has the advantages that clinical effectiveness and safety of a TCR technology are improved, and fussy experimental operations are also avoided.

The invention discloses a solar cell silicon wafer cutting parameter prediction method based on a neural network, which relates to the technical field of the neural network. The method comprises the following steps: collecting sample data, selecting different cutting parameters, cutting the silicon ingot with a cutting machine, and measuring the surface roughness of the silicon wafer; training theneural network according to the appropriate parameters and network structure, and continuously updating the weights of each neuron according to the error value, and achieving the optimal matching ratio until the error reaches the limited standard; performing parameter prediction through a prediction model, comparing the relationship between surface roughness and cutting parameters, and finding the optimal configuration of cutting parameters corresponding to surface roughness. The method of the invention can establish a neural network prediction model and carry out parameter prediction by relying on a small amount of sample data, and according to the prediction result, the optimal cutting parameters can be selected on the premise of considering low roughness and fast cutting.

The invention belongs to the technical field of biology, and relates to a method for quantitatively and traceably studying mussel mucoprotein degradation. The method comprises the steps that 1, aftermussel mucoprotein is combined with radioactive elements or a fluorescent reagent, a polymer is formed through intermolecular cross-linking, or the mussel mucoprotein covers a biomedical material to be cured, and mussel mucoprotein microballoon spheres are formed; 2, an in-vitro model and an in-vivo model are built; 3, by means of the in-vivo or/and in-vitro model, increase of a marker in supernate and reduction of the marker on the mussel mucoprotein polymer/covered microballoon spheres are qualitatively and quantitatively measured, and therefore the degradation characteristic and degradation rate of the mussel mucoprotein in different environment are determined. According to the method, the defect of the biomedical macromolecule degradation research methodology is overcome, and the study method is dynamic and quantitative.

The invention relates to a method for preparing and separating surfactants which have high biological safety and contain amino terminal and diamino. The method comprises the following steps: adding fatty acid solids, 1-(3-dimethylaminopropyl)-3-vinyl carbodiimide solid and 1-hydroxybenzotriazole solids into dichloromethane, and dissolving to obtain a mixed solution; dropwise adding the mixed solution into 1,2-(2-aminoethoxy) ethane, reacting in an ice-salt bath, pouring the obtained reaction solution into saturated salt water, and performing suction filtration on the separated jelly-shaped small molecule gel, thus obtaining a mixture of the surfactants containing amino terminal and diamino; and adding the obtained mixture into a chromatographic column filled with silica gel, eluting by using gradient mixed eluant of dichloromethane and methanol, and separating the mixture of the surfactants containing the amino terminal and diamino, thus respectively obtaining a surfactant containing the amino terminal and a surfactant containing the diamino.