Novel stimulating electrode based in-vitro brain slice electrical signal recording device

Abstract

The invention discloses a novel stimulating electrode based in-vitro brain slice electrical signal recording device which comprises a brain slice recording slot, a brain slice storage slot, a gas supply device, a perfusing drug-delivery device, a temperature control device, an electrode fixing and operating device, a neural network signal recording device and a dissecting microscope. Two microelectrodes are fixed to the electrode fixing and operating device and are inserted into a feeding groove from a top cover of the brain slice recording slot, the other end of each microelectrode is connected with the neural network signal recording device through a lead, the two microelectrodes are a stimulating microelectrode and a recording microelectrode respectively, and the stimulating microelectrode is an epoxy resin/graphene/nano-copper composite material electrode. The novel stimulating electrode based in-vitro brain slice electrical signal recording device effectively overcomes the defect that an old in-vitro brain slice recording device cannot automatically control the temperature, is provided with a smaller feeding groove, is inconvenient to use and makes records inaccurate and does not efficiently stimulate microelectrodes due to shake caused by various situations.

Description

technical field [0001] The invention belongs to the technical field of local preservation and testing of human or animal tissues, and in particular relates to an electrical signal recording device for isolated brain slices based on a novel stimulating electrode. Background technique [0002] Gamma neural oscillation is a 30-80Hz synchronous signal that exists in many areas of the human and animal brains. Studying neural oscillations can understand the neural characteristics of animal and human cognition, learning, memory, excitement and arousal, and is a hotspot in medical and psychological research. Currently, known isolated neural network oscillation techniques include brain slice recording devices, brain slice recording tanks, gas supply devices, perfusion drug delivery devices, lighting and temperature control devices, electrode fixation and manipulation devices, neural network signal recording devices, shielding And grounding device and system shockproof device. After...

AI Technical Summary

Problems solved by technology

[0003] The technical problem solved by the present invention is to provide an isolated brain slice electrical signal recording device based on a new type of stimulating electrode. This system overcomes the inability of the old isolated brain slice recording device to automatically control temperature, small incubation tank, and inconvenient use. , Inaccurate recording due to vibration caused by various situations, and lack of efficient stimulation of microelectrodes and other defects

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