The invention relates to an isolated organ perfusion system, which comprises a liquid storage container, a centrifugal pump, a membrane lung, a first peristaltic pump, a leukocyte filter, a hemodialyzer and a waste liquid bag, wherein the liquid inlet end of the first peristaltic pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the first peristaltic pump is communicated with the liquid inlet end of the hemodialyzer through a pipeline, the liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, the waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path, and the leukocyte filter is arranged on the filtering circulation auxiliary path. According to the invention, when the leukocyte filter is blocked due to long-time work,the leukocyte filter can block the filtering circulation auxiliary path and cannot block the perfusion circulation main path, the perfusion circulation main path can still continue to work at the moment, the in-vitro organs can be continuously protected, and the leukocyte filter on the filtering circulation auxiliary path can be replaced in time.