Modular animal digestive tract in-vitro simulation system and human intestinal tract simulation method thereof

Abstract

The invention discloses a modular animal digestive tract in-vitro simulation system and a human intestinal tract simulation method thereof. The modular animal digestive tract in-vitro simulation system comprises at least one animal digestive tract in-vitro simulation unit. Each animal digestive tract in-vitro simulation unit includes a frame, a reaction tank used for simulating a specific digestive tract cavity, and multiple function modules which can complete a specific and relatively independent task of intestinal tract simulation. Each frame is provided with multiple installation positions for installing the function modules and the reaction tank, and the function modules and the reaction tank are installed on the frame detachably. Each function module includes a sensor or a moving part, or includes both the sensor and the moving part. Each function module uses a separate Arduino nano 328 as a control chip, and is connected with an upper computer through a USB serial port. Each frame is equipped with a USB active hub. A USB is responsible for power supply to single chip microcomputers in the function modules and instruction transmission between upper and lower computers. Multiple function modules are integrated in one frame, so that the lab space is saved, the modules can be replaced rapidly, and a faulty module can be checked and replaced without shutdown.

Description

technical field [0001] The invention relates to the technical field of in vitro simulation of animal digestive tract, in particular to a modular animal digestive tract in vitro simulation system and a human intestinal simulation method thereof. Background technique [0002] The continuous promotion of human and animal digestive tract in vitro simulation devices has provided great convenience for the research of food and feed science, human and animal nutrition, and has gradually been accepted by the academic community. Some simulation devices can even be used as production equipment. Production needs Certain active substances activated by the intestinal environment or enterobacteria. [0003] There are many similarities in the design of the published digestive tract simulation devices: the core components are one to several sealed reaction containers, which are used to simulate specific parts of the digestive tract. A limited number of equipment, such as constant temperatur...

AI Technical Summary

Problems solved by technology

[0004] However, the following problems exist in the research and development of the in vitro digestive tract simulation device at this stage: 1. The research in this field is still in its infancy and is not yet mature.

However, the existing device structures are relatively closed, without replacement and upgrade design, and lack of scalability, which will cause difficulties for future model improvement and evolution

2. There is no model or device that is relatively mainstream or widely recognized. Different research teams often only have models developed and built by themselves, and cannot build or purchase models used by other research groups for comparison or verification. Therefore, each model Both lack the horizontal verification conditions of third-party laboratories

Reliability and reproducibility of research using simulators are compromised, negatively affecting the deployment of such devices

3. Even if different research teams use the same model, the differences may accumulate due to the different hardware used to build the simulation device, until it affects the repeatability of the experimental results

4. The software and hardware of the few devices that have been released abroad that can realize program control are strictly closed development, and the software is only aimed at a specific hardware system to complete a single task.

If it is necessary to modify or update the hardware, the existing software cannot support it and must be redeveloped, resulting in a waste of resources

5. None of the domestic existing patents for analog devices describe in detail the specific implementation methods of software control, let alone the specific implementation methods of software and hardware extensions

6. Existing devices cannot save and share settings and experimental parameters, and still need to manually input multiple parameters

7. The equipment that constitutes most of the current simulation devices, such as peristaltic pumps, magnetic stirrers, etc., are not specially designed for digestive tract simulation work, and there are many defects in function

8. The equipment that constitutes most of the existing simulation devices, such as peristaltic pumps, magnetic stirrers, and pH automatic controllers, has not been optimized in terms of appearance, volume and other parameters. Not only are the connections difficult, the control is scattered, and it takes up a huge space, some Even need to occupy the entire laboratory bench

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