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Simple micro-fluidic chip and cell analysis method

A microfluidic chip, a simple technology, applied in the direction of analytical materials, biochemical equipment and methods, tissue cell/virus culture devices, etc., can solve the problems of complicated cell culture operations, cell damage, and inability of cells to obtain nutrients. Achieve the effect of avoiding perfusion equipment, reducing processing cost and difficulty, and simplifying the complexity of chip structure

Inactive Publication Date: 2016-03-23
NEIJIANG NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the fluid shear force generated by the flow of the culture medium will cause certain damage to the cells, and at the same time, the interface between the microfluidic chip and the peripheral fluid circuit makes the cell culture operation complicated.
Another method of cell culture in microchannels is to use static culture, which is the most commonly used method for cell culture. Although this method does not cause damage to cells, it applies static culture technology to microchannels with limited space. Diffusion cells often do not get enough nutrients

Method used

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  • Simple micro-fluidic chip and cell analysis method
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  • Simple micro-fluidic chip and cell analysis method

Examples

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preparation example Construction

[0050] The preparation method of the microfluidic chip in this embodiment:

[0051] Firstly, three layers of photosensitive dry film are sequentially laminated on a glass plate through a laminating machine so that the thickness of the photosensitive layer is about 100 μm, and the microchannel pattern is printed on a transparent film by an Epson inkjet printer to make a photomask, through which ultraviolet light is transmitted. The mask is irradiated on the photosensitive dry film for 70s, and developed with 1% sodium carbonate to make a microchannel male mold; the prepolymer obtained by mixing the PDMS matrix and curing agent by weight 10:1 is cast on the microchannel male mold, and vacuumized to remove Bubbles, cured at 60°C for 3h. The cured PDMS substrate was peeled off from the photosensitive dry film positive mold, and a flat hole punch (5 mm in diameter) was used to punch holes on both sides of the microchannel to form a liquid storage pool, and oxygen plasma surface tre...

Embodiment 3

[0053] Example 3 Microfluidic Chip Cell Analysis Method: Taking the Analysis of the Apoptotic Effect of the Natural Anticancer Drug Saponin I on Human Lung Cancer Cell A549 as an Example

[0054] Operate with the microfluidic chip prepared in Example 2.

[0055] Paprika saponin I is a natural drug molecule that has been proven to have anticancer effects. On this basis, this example takes the analysis of the apoptosis-promoting effect of papaya saponin I on human lung adenocarcinoma A549 as an example to verify the invented microfluidic chip cell analysis technology.

[0056] Experimental materials and equipment involved in the following examples:

[0057] Materials include: saponin I (content 98.36%) purchased from Shanghai Chunyou Biotechnology Co., Ltd., dissolved in DMSO, and stored at 4°C; human laminin (Prospec, USA); dextran (70kDa) (Shanghai Sangong ); LIVE / DEADViability / Cytotoxicity Kit (Molecularprobes, USA); Lactate Dehydrogenase Cytotoxicity Detection Kit, Streppe...

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Abstract

The invention belongs to the technical field of a micro-fluidic chip, and in particular relates to a simple micro-fluidic chip for cytological analysis and a cell analysis method. The simple micro-fluidic chip comprises a basal layer and a cell culture layer which is arranged on the basal layer, wherein liquid storage tanks, which are located in a same plane, are arranged on the cell culture layer; a micro-channel is additionally arranged on the cell culture layer; and the bottoms of the at least two liquid storage tanks communicate by virtue of the micro-channel. The simple micro-fluidic chip is applied to cell culture and cytological analysis is carried out on cells cultured in the micro-channel of the micro-fluidic chip; and compared with an existing micro-fluidic chip, the micro-fluidic chip disclosed by the invention is easier to be adopted by general biology laboratories for processing and for the operations of cell culture and cell analysis.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a simple microfluidic chip for cytological analysis and a cell analysis method. Background technique [0002] Cells are the structure and life activity unit of living organisms. Cell proliferation, differentiation, migration, and metabolism are closely related to important physiological and pathological processes such as embryonic development, angiogenesis, tissue repair, immune defense, and tumorigenesis. Research is the foundation of modern life science research. [0003] In recent years, it has been reported that microfluidic chip technology is applied to cell research, which provides a new method and platform for cell research. Microfluidic chip provides a new technical platform for cell research, and its unique advantages include: microfluidic channel size similar to cells; effective cell microenvironment control and microenvironment simulation in cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12Q1/02
CPCC12M23/16C12Q1/02G01N33/5008
Inventor 霍峰
Owner NEIJIANG NORMAL UNIV
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