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A degradation rate and feather technology, applied in the field of feather degradation rate determination, can solve the problems of poor parallelism and repeatability of measurement, low measurement efficiency, inaccurate detection results of weight loss method, etc., achieves good parallelism and repeatability, and improves efficiency. , the effect of reducing the difficulty of the experiment
Inactive Publication Date: 2018-09-18
NORTHEAST AGRICULTURAL UNIVERSITY
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[0003] In order to solve the problems of inaccurate detection results of the existing weight reduction method, poor measurement parallelism and repeatability, and low measurement efficiency, the invention provides a method for measuring feather degradation rate, and the adopted technical scheme is as follows:
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Embodiment 1
[0027] The present embodiment provides a kind of method of measuring feather degradation rate, and this method comprises the steps:
[0028] 1) Mark 30 400-mesh nylon bags (6cm*5cm) with a pencil and dry them at 105°C to constant weight. Weigh each nylon bag and record it as M1;
[0029] 2) Wash and dry the feathers, and after crushing, pass through a 30-mesh sieve and then a 40-mesh sieve to obtain feather powder (about 500 μm);
[0030] 3) Weigh 2g of feather powder, place it in the nylon bag of step 1), seal it and dry it at 105°C to constant weight, weigh it separately, and record it as M2;
[0031] 4) After the nylon bags are sterilized, at first, get 10 nylon bags and completely immerse in 10 parts of MRS medium, then inoculate feather-degrading bacteria (equivalent to 10 parallel treatments) in 10 parts of MRS medium; secondly, Take 10 nylon bags and submerge them completely in 10 parts of liquid LB medium, then inoculate Escherichia coli (equivalent to 10 parallel tre...
Embodiment 2
[0040] The present embodiment provides a kind of method of measuring feather degradation rate, and this method comprises the steps:
[0041] 1) Mark a 400-mesh nylon bag (6cm*5cm) with a pencil and dry it at 105°C to a constant weight. Weigh each nylon bag and record it as M1;
[0042] 2) Wash and dry the feathers, and after crushing, pass through a 30-mesh sieve and then a 40-mesh sieve to obtain feather powder (about 500 μm);
[0043] 3) Weigh 2g of feather powder, place it in the nylon bag of step 1), seal it and dry it at 105°C until constant weight, weigh it, and record it as M2;
[0044]4) After the nylon bag is sterilized, place the nylon bag in the liquid MRS medium, and inoculate the lactococcus isolated and screened by our laboratory, so that the medium is completely submerged in the nylon bag, and ferment under the optimum growth conditions of microorganisms. After completion, remove the nylon bag and place it in a container, then add 500mL of distilled water to co...
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Abstract
The invention discloses a method for detecting the feather degradation rate, and belongs to the technical field of feather degradation rate detection methods. The method comprises the following steps:drying a nylon bag to a constant weight, weighing the weight of the nylon bag, and recording the weight as M1; cleaning feathers, drying the feathers, crushing the feathers, and sieving the crushed feathers to obtain feather powder; weighing the feather powder, placing the feather powder in the nylon bag, sealing the nylon bag, drying the nylon bag containing the feather powder to a constant weight, weighing the weight, and recording the weight as M2; immersing the nylon bag in a liquid medium, inoculating the liquid medium with a bacterial liquid, performing fermentation, taking out the nylon bag after the fermentation, adding distilled water to immerse the nylon bag, placing a container in a water bath shaker, performing cleaning, repeating above steps multiple times, drying the cleanednylon bag, weighing the weight of the nylon bag, and recording the weight as M3; and calculating the feather degradation rate according to a formula. The method has the advantages of accuracy in thedetection result of the feather degradation rate, good parallelism and repeatability, simplicity in operation, and high detection efficiency, is suitable for evaluating the ability of microorganisms to degrade feathers, or is used for studying the degradation of feathers by the microorganisms.
Description
technical field [0001] The invention relates to a method for measuring the degradation rate of feathers, and belongs to the technical field of detection methods for the degradation rate of feathers. Background technique [0002] Feathers are rich in keratin, which is a good source of animal protein feed, but keratin is not easily digested by livestock and poultry, and the utilization rate is reduced. Therefore, before the feathers are fed, methods need to be taken to degrade the keratin of the feathers, and the biological fermentation method is the development direction of degrading the feathers to improve their feeding value. But there is no uniform experimental method for detecting feather degradation rate at present. At present, the commonly used experimental method for measuring the degradation rate of feathers is the weight loss method, but the traditional detection method places the whole feather in the fermentation broth, causing the broken feathers that have not bee...
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