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Three-dimensional cell scaffold printing method based on cell state feedback

A technology of three-dimensional cells and cell states, applied in the field of cell printing, can solve problems such as experimental result deviation, cell death, and scaffold structure problems, and achieve the effect of avoiding experiments, shortening research cycle and cost, and detecting stem cell differentiation in real time

Active Publication Date: 2014-09-17
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Under the influence of many external factors, it is likely to cause premature cell death. Such a result does not prove that there is a problem with the structural formula of the scaffold itself, and it will cause deviations in the experimental results.
Bias in experimental results can lead to more ineffective iterative scaffolding processes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Cell culture part:

[0032] (I) Prepare a neutral red staining solution, dissolve 0.1g of Jianna Green B powder in 1L of normal saline and stir evenly to prepare a Jianna Green B solution with a concentration of 0.01%.

[0033] (II) Take 100ml of the above solution and immerse it in a petri dish, soak the skeletal stem cells for 1 hour, then filter out the skeletal stem cells through filter paper.

[0034] (III) In the filtered skeletal stem cells, separate them by centrifuge, select the cells that precipitate green, and separate the colorless cells. The green cells are living bone stem cells. Then add cell culture medium to configure 100ml cell green solution.

[0035] (2) Gel bracket part:

[0036] The invention adopts seaweed glue material as the basic material of the scaffold, and mixes phenolphthalein to detect the alkaline phosphatase secreted by osteoblasts in the early stage.

[0037] (1) Dissolve 4 g of sodium alginate in 100 ml of deionized water to pr...

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Abstract

The invention discloses a three-dimensional cell scaffold printing method based on cell state feedback. According to the invention, a marker is added during cell sap culture, so that the purpose of real-time tracking of cell distribution is realized; in a process of manufacturing a support material, test solutions for detecting cell secretion are mixed, so that the purpose of real-time detection of a differentiation process of stem cells is realized. Through the cell tracking and the real-time detection of the cell differentiation process, real-time monitoring of sedimentation of gelatin carrying cells is realized, so that the study of a direct relation between cell survival and differentiation and all parameters (such as deposition velocity and piezoelectric pulse width) of the sedimentation technology is facilitated, thereby greatly shortening the study period and reducing the cost.

Description

technical field [0001] The invention relates to the field of cell printing, in particular to a three-dimensional cell scaffold printing method based on cell state feedback. Background technique [0002] Rapid prototyping (RP) technology is an advanced manufacturing technology developed in the 1990s. Under the control of the computer, the RP system can make a section with a certain small thickness and a specific shape each time according to the shape of the part, and then They are bonded layer by layer to obtain the three-dimensional parts that need to be manufactured. The RP systems manufactured by different companies use different forming materials, and the working principles of the systems are also different, but the basic principles are the same, that is, "layer-by-layer manufacturing and layer-by-layer superposition". Using rapid prototyping (RP) technology, you can directly accept product design (CAD) data without preparing any molds, tools and fixtures, and quickly ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N11/10C12N11/04
Inventor 贺永夏冰傅建中赵朋金育安
Owner ZHEJIANG UNIV
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