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Application of salvia miltiorrhiza formula granules in preparing medicine for preventing and treating AIDS (acquired immune deficiency syndrome) encephalopathy

A technology of Danshen formula granules and AIDS encephalopathy, which is applied in the application field of drugs for the prevention and treatment of nerve damage caused by HIV-1 Tat protein, and achieves a good therapeutic effect

Inactive Publication Date: 2014-06-11
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no related reports about the protection of Danshen Formula Granules on nerve damage caused by HIV-1 Tat protein or its application in HAND treatment.

Method used

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  • Application of salvia miltiorrhiza formula granules in preparing medicine for preventing and treating AIDS (acquired immune deficiency syndrome) encephalopathy
  • Application of salvia miltiorrhiza formula granules in preparing medicine for preventing and treating AIDS (acquired immune deficiency syndrome) encephalopathy
  • Application of salvia miltiorrhiza formula granules in preparing medicine for preventing and treating AIDS (acquired immune deficiency syndrome) encephalopathy

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Embodiment 1

[0028] (1) Main reagents, cells and instruments

[0029] HIV-1 Tat protein was purchased from Shenzhen Baiwansen Bio-Trading Co., Ltd. (Prospec, USA, batch number: 212PCLADEB28); traditional Chinese medicine Danshen formula granules were purchased from Guangdong Yifang Pharmaceutical Co., Ltd. (product batch number: 1002014; prepared with sterile water) required concentration). Rat adrenal pheochromoma cell line (PC12) was purchased from Shanghai Institute of Cells, Chinese Academy of Sciences. MTT was purchased from Sigma (batch number: MTBF7873V); LDH (lactate dehydrogenase) detection kit was purchased from Nanjing Jiancheng Bioengineering Research Institute; Tunel detection kit was purchased from Promega, USA (batch number: 0000073091). Inactivated fetal bovine serum (batch number: 111023) was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; horse serum (batch number: 841843), DMEM medium (batch number: 823186) and MEM medium (batch number: 793870) were...

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Abstract

The invention belongs to the technical field of pharmacy, and in particular relates to an application of salvia miltiorrhiza formula granules in preparing medicine for preventing and treating nerve injury caused by AIDS (acquired immune deficiency syndrome) encephalopathy and HIV-1Tat (human immunodeficiency virus-1Tat) proteins. According to the application an HIV-1 Tat protein injured neural cell line PC12 and a neural cell on a mouse brain slice are protected with the salvia miltiorrhiza formula granules in different concentrations, and the effect of the salvia miltiorrhiza formula granules in reducing neural cell death due to Tat protein is respectively detected by an MTT (thiazolyl blue) experiment, LDH (lactate dehydrogenase) detection and a Tunel experiment; and the effect of the salvia miltiorrhiza formula granules in reducing Tat protein injury neural cell synaptic connection function is detected by using Dil golden granule diOlistic labeling dendritic spine. Statistic analysis on experiment results discover that the salvia miltiorrhiza formula granules have a good protecting effect to neural cell injury caused by Tat protein, and can be used for preparing a medicine for preventing and treating AIDS encephalopathy and Tat protein.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and specifically relates to a new application of traditional Chinese medicine Danshen formula granules, that is, the application of the traditional Chinese medicine in the preparation of drugs for the prevention and treatment of AIDS encephalopathy, especially in the prevention and treatment of nerve damage caused by HIV-1 Tat protein Pharmaceutical applications. Background technique [0002] AIDS encephalopathy, now also known as HIV-associated neurocognitive disorders (HAND, HIV-associated neurocognitive disorders), is characterized by movement, behavior and cognitive impairment, and has recently been divided into three forms according to the severity of the disease: asymptomatic neurocognitive disorders Cognitive impairment (Asymptomatic neurocognitive impairment, ANI), mild neurocognitive disorder (mild neurocognitive disorder, MND) and HIV-associated dementia (HIV associated dementia, HAD)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61K9/16A61P31/18A61P25/28
Inventor 刘红亮王靖凯石贞玉胡磊刘彬邓锦波徐括喜
Owner HENAN UNIVERSITY
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