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Wintergreen pure polysaccharide LTC-0-1 and application thereof

A kind of staghorn grass, pure polysaccharide technology, applied in the application, medical preparations containing active ingredients, food preparation and other directions

Active Publication Date: 2012-12-26
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When we used the GFP green fluorescent protein transgenic mouse model and the rat pheochromocytoma PC12 cell line model to carry out research on substances with neurotrophic effects in ethnic medicines, we screened wintergreen polysaccharide LTC-0-1, which can make GFP small The average length of neurites in rat hippocampal neurons increases, the number of neurites increases, the density of dendritic spines increases significantly, and the expression of neuronal postsynaptic membrane proteins increases, which promotes neuronal maturation and other pathways to exert their neurotrophic activity; this result has not been seen at home and abroad. Related reports

Method used

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  • Wintergreen pure polysaccharide LTC-0-1 and application thereof
  • Wintergreen pure polysaccharide LTC-0-1 and application thereof
  • Wintergreen pure polysaccharide LTC-0-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of wintergreen crude polysaccharide

[0032] Dry the whole herb of Wintergreen, add 80% alcohol to reflux and extract 3 times, 2 hours each time. The residue after alcohol extraction was evaporated to remove ethanol, and extracted in a boiling water bath with distilled water for 3 times, each time for 2 h. The combined water extracts were concentrated under reduced pressure, precipitated with ethanol, the final concentration of ethanol was not lower than 80%, and the precipitate was vacuum-dried to obtain crude wintergreen polysaccharide.

Embodiment 2

[0033] Embodiment 2, the preparation of pure polysaccharide LTC-0-1

[0034] The crude polysaccharide was chromatographed on DEAE-cellulose column, eluted with distilled water, developed by sulfuric acid-phenol method, and the polysaccharide part was collected to obtain pure polysaccharide. The pure polysaccharide eluate was concentrated to a small volume, separated by Sephacryl S-300 HR column chromatography, 0.2 mol / L sodium chloride solution was used as the eluent, the flow rate was 0.8 mL / min, each tube was automatically collected for 10 min, and the sulfuric acid-phenol method was used For detection, at a wavelength of 490nm, the absorbance was plotted against the number of collection tubes, and two single peaks were collected, one of which was freeze-dried to obtain the polysaccharide component LTC-0-1.

Embodiment 3

[0035] Example 3, the purity, physical and chemical properties, spectral analysis, gas chromatography analysis and molecular weight of the polysaccharide component LTC-0-1

[0036]The polysaccharide component LTC-0-1 is proved to be a polysaccharide structure by conventional methods such as total hydrolysis, molecular weight determination, nuclear magnetic resonance, infrared spectroscopy and other chemical and spectroscopic methods.

[0037] (1) Confirmation of the purity of polysaccharide components

[0038] The polysaccharide component LTC-0-1 obtained above was analyzed for purity by Sephacryl S-300 HR gel column chromatography with 0.2 mol / L sodium chloride solution as the mobile phase at a flow rate of 0.5 mL / min. The polysaccharide component LTC- 0-1 is a single symmetrical peak. Gel permeation chromatography (GPC) analysis, 0.1 mol / L phosphate buffer (pH 7.8 -8.0), the same result.

[0039] (2) Physicochemical properties of wintergreen polysaccharide LTC-0-1 componen...

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Abstract

The invention provides a wintergreen pure polysaccharide LTC-0-1 and an application thereof, which belong to the field of medicines and healthcare foods. The invention discloses an extracting method of the wintergreen pure polysaccharide LTC-0-1, a composition of the LTC-0-1 and a molecular weight of the LTC-0-1; it is proved by experiments that the LTC-0-1 can be used for increasing average lengths of hippocampal neurons of a GFP green fluorescent protein transgenic mouse, increasing the number of the neurons, obviously increasing dendritic spine densities of neuron dendrons in a unit length, increasing neuronal post-synaptic membrane protein expression and promoting the neurons to be more mature; the LTC-0-1 can be also used for inducing neuron-like differentiation of PC12 cells, promoting enation of the cells, enhancing activity of cell acetylcholinesterase AchE and increasing expression index of cell growth related protein GAP-43 and obtains neurotrophic activity; and the LTC-0-1 shows a growth promoting effect, a differentiation effect, a maturing effect and a damaged nerve cell function recovery effect on nerve cells and has broad prospects in development and application in the technical field of medicines and healthcare foods.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food development, and relates to pure wintergreen polysaccharide LTC-0-1 which is a traditional Chinese medicine wintergreen extract and its application in medicine and health food. Background technique [0002] wintergreen Pyrola corbieri Levl is a plant of the Wintergreen family Wintergreen. It is used in the folk to treat rheumatic joint pain, kidney deficiency low back pain, waist and knee weakness, cough due to fatigue, traumatic bleeding, carbuncle sores, snake bites, etc. Modern studies have shown that wintergreen has obvious antibacterial, anti-inflammatory, immune-stimulating, and improving coronary heart disease symptoms (Zheng Huzhan, Dong Zehong, She Jing. Modern Research and Application of Traditional Chinese Medicine. Volume 6. Beijing: Xueyuan Press, 1999:5844; Li Dong, Yang Song, Song Baoan, et al. Research on Wintergreen. Journal of Guizhou University: Natural Science...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P25/28A61P9/10A23L1/09
Inventor 杨娟莫正昌邓靖吴兰芳彭梅
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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