Rat brain bilateral optical fiber implanting device

Abstract

The invention relates to the field of experimental instruments, and discloses a mouse brain bilateral optical fiber implantation device which comprises an operation platform and further comprises an optical fiber clamping mechanism and a mouse head restraining mechanism which are installed on the operation platform, the optical fiber clamping mechanism is used for clamping and fixing an optical fiber, and the mouse head restraining mechanism is used for fixing the head of a mouse; the optical fiber clamping mechanism comprises a lifting platform installed on an operation platform; the vertical sliding block is arranged on the lifting platform, and the lifting platform is connected with the vertical sliding block through a driving component. An optical fiber can be directly and rigidly clamped through the optical fiber fixator of the optical fiber clamp, the horizontal position and the inclination angle of the optical fiber can be locked through the connection sliding block self-locking piece and the connection sleeve fastening piece of the dial, and it is ensured that the optical fiber is kept in a stable posture in the whole implanting process; the outer surface of the horizontal guide rod is marked with a symmetrical dial, and the horizontal distance between the optical fibers on the two sides can be accurately controlled.

Description

Technical Field

[0001] This invention relates to the field of experimental equipment technology, and in particular to a bilateral fiber optic implantation device for the rat brain. Background Technology

[0002] To study brain mechanisms, scientists typically conduct experiments using live mice. This involves specifically targeting neurons and combining viral tracing and optogenetics to explore the long-range circuit connections and functions of neurons in different subregions of the cerebral cortex. Optogenetics utilizes light-sensitive proteins and genetic engineering to precisely control the activity of specific cells through light exposure. Its core involves introducing light-sensitive channel protein genes into target cells, activating or inhibiting them under specific wavelengths of light, thereby studying or intervening in biological functions. In practice, one end of an optical fiber is implanted within the target neuron to transmit light signals. Because the brain is bilaterally symmetrical, it is often necessary to simultaneously inject viruses and implant optical fibers in the same brain region on both sides of the same experimental animal to achieve the effect of regulating neuronal activity in that brain region.

[0003] A search revealed that Chinese invention patent publication number CN116392727A discloses an implantable fiber optic device, comprising a laser generator, a first optical fiber connected to the output interface of the laser generator, and a second optical fiber connected to the end of the first optical fiber away from the laser generator. The end of the second optical fiber away from the first optical fiber is implanted into the deep subcortical region of an experimental animal via an implantation component. When the laser transmitter is activated, the end of the second optical fiber away from the first optical fiber emits a light source of a set wavelength to excite a photosensitive dye in the deep subcortical region.

[0004] The following problems exist when using fiber optic implantation devices to fix optical fibers: When symmetrically embedding optical fibers, it is necessary to first fix one side of the optical fiber with dental cement. When the two target points are close to each other, the dental cement will overflow everywhere when it is in liquid state. The dental cement at one embedding point will interfere with the next embedding point, causing inconvenience in operation. If dental cement is not used for fixation, the optical fiber embedded first may be displaced due to operation. Summary of the Invention

[0005] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a bilateral optical fiber implantation device for the mouse brain, which solves the problem of inconvenient optical fiber placement at similar locations in existing technologies.

[0006] (II) Technical Solution To achieve the above objectives, the present invention is implemented through the following technical solutions.

[0007] A bilateral fiber optic implantation device for mouse brain includes an operating platform, and also includes a fiber optic clamping mechanism and a mouse head restraint mechanism installed on the operating platform. The fiber optic clamping mechanism is used to clamp and fix the fiber optic cable, and the mouse head restraint mechanism is used to fix the head of the mouse. The fiber optic clamping mechanism includes: A lifting platform, installed on the operating platform; A vertical slider is installed on the lifting platform. The lifting platform is connected to the vertical slider through a drive component. The vertical slider is set to move along the vertical direction of the lifting platform. A horizontal guide rod is mounted on a vertical slider, and the outer surface of the horizontal guide rod is marked. Two dials are symmetrically arranged on the horizontal guide rod; Two fiber optic clamps are mounted on two corresponding dials via a connecting shaft. The fiber optic clamps are used to hold the fiber optic cable.

[0008] In one embodiment, the lifting platform includes: The bracket mounting base is installed on the operating platform; The damping shaft is rotatably mounted on the bracket mounting base; The damping shaft is provided with a first support rod and a second support rod that are parallel to each other. The first support rod and the second support rod form a slide rail. A vertical slider is connected to the slide rail. The bottom and top of the slide rail are respectively provided with a lower bracket and an upper bracket for connecting the two ends of the first support rod and the second support rod. The lower end of the first support rod passes through the lower bracket and is connected to the damping shaft.

[0009] In one embodiment, the driving component includes: A vertical threaded rod passes through the upper bracket. The lower end of the vertical threaded rod extends between the first support rod and the second support rod. The upper end of the vertical threaded rod is connected to a knob for rotation. Rotating the knob can drive the vertical slider to move along the vertical direction of the lifting platform through the vertical threaded rod.

[0010] Preferably, the dial includes: The connecting slider is slidably connected to the outer surface of the horizontal guide rod, and the connecting slider is locked to the surface of the horizontal guide rod by a self-locking component. A disc is mounted on a connecting slider. One side of the disc is provided with angle indicator scales distributed in a circumferential array. The connecting shaft is mounted on the center of the disc. The connecting sleeve is mounted on the fiber optic clamp at one end and rotated and sleeved on the connecting shaft at the other end. Fasteners are provided on the side of the connecting sleeve to limit the angle of the connecting sleeve. The fiber optic retainer is installed at the end of the connecting sleeve away from the disc. The fiber optic retainer is used to clamp and fix the fiber optic cable. An angle pointer is mounted on the outer surface of the connecting sleeve. The angle pointer is attached to the disc and is matched with the angle indication scale.

[0011] In one embodiment, it also includes: The limit mechanism is installed on the operating platform; A mouse support mechanism is installed on the operating platform, and the limiting mechanism is used to support and limit the mouse support mechanism; The scalp-supporting mechanism, mounted on the limiting mechanism, is used to support the mouse's scalp.

[0012] In one embodiment, the limiting mechanism is a U-shaped bracket, the mouse support mechanism is embedded in the U-shaped bracket, the mouse head restraint mechanism and the scalp spreading mechanism are both installed on the top of the U-shaped bracket, the closed end of the U-shaped bracket faces the fiber optic clamping mechanism, the bottom of the U-shaped bracket is provided with a support leg, and the support leg is connected to the operating platform, the top of the U-shaped bracket is provided with a limiting groove, and the bottom wall of the limiting groove is provided with a locking hole.

[0013] In one embodiment, the mouse support mechanism is a mouse tray, which is set in the middle of a U-shaped support. The bottom of the mouse tray is provided with tray legs, so that the mouse tray and the U-shaped support are on the same plane. A connecting wing plate extends from the side of the mouse tray near the limiting groove. The connecting wing plate is embedded in the limiting groove. A straight groove hole is opened on the connecting wing plate, which corresponds to a locking hole. A bolt is provided in the straight groove hole, and the connecting wing plate is connected to the U-shaped support by the bolt.

[0014] In a preferred embodiment, the walls on both sides of the mouse tray are symmetrically provided with several restraint strap fixing grooves for the restraint straps to pass through.

[0015] In one embodiment, the mouse head restraint mechanism is a fixed base, which is located on the top of a U-shaped bracket. A support guide rod is vertically arranged on the top of the fixed base, and an ear rod connector that can slide up and down is provided on the support guide rod. The ear rod connector is provided with a limiting ear rod for fixing the mouse's head.

[0016] In one embodiment, the scalp stretching mechanism is a guide support frame, which is located on top of a U-shaped bracket. The guide support frame has an internal slide rail containing a guide slider. One end of the guide slider extends to the outside of the guide support frame and is fitted with a support sleeve. A horizontally adjustable scalp traction rod passes through the support sleeve. One end of the scalp traction rod is configured as a hook. A height adjuster is vertically mounted on the top of the guide support frame, with the lower end of the height adjuster passing through the guide slider. Rotating the height adjuster drives the guide slider to move up and down along the guide support frame.

[0017] (III) Beneficial Effects The bilateral fiber optic implantation device for the mouse brain of the present invention has the following beneficial effects: The fiber optic clamp can directly and rigidly hold the fiber optic cable. With the help of the dial-mounted connecting slider self-locking component and the connecting sleeve fastener, the horizontal position and tilt angle of the fiber optic cable can be locked respectively, ensuring that the fiber optic cable maintains a stable posture throughout the implantation process. At the same time, the marking on the outer surface of the horizontal guide rod and the symmetrical dial design can precisely control the horizontal distance between the two fibers. Even if the two target points are close to each other, the positioning boundaries of the two fibers can be clearly distinguished by quantitative adjustment, avoiding target point confusion caused by cement overflow. The scalp opening mechanism symmetrically opens the scalp with traction rods, clearly exposing the skull target area and further reducing operation obstruction.

[0018] The damping shaft of the lifting platform can stably adjust the slide rail angle. Together with the parallel slide rail composed of the first support rod and the second support rod, it provides bidirectional guidance for the vertical slider, ensuring that the optical fiber is always aligned with the target point when it is tilted during implantation. The vertical threaded rod of the drive component realizes the micro-displacement adjustment of the vertical slider through thread transmission. The threaded structure has a self-locking characteristic. After adjustment to the target depth, no additional locking is required, which can prevent the optical fiber from being deviated in depth due to gravity or vibration. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a perspective view of the present invention.

[0021] Figure 2 This is a top view of the present invention.

[0022] Figure 3 This is the right view of the present invention.

[0023] Figure 4 This is a side view of the present invention.

[0024] Figure 5 This is a schematic diagram of the optical fiber clamping mechanism of the present invention.

[0025] Figure 6 This is a schematic diagram of the connection of the dial of the present invention.

[0026] Figure 7 For the present invention Figure 6 Enlarged view of the structure at point A in the middle.

[0027] Figure 8This is a schematic diagram of the assembly of the dial and the optical fiber clamp of the present invention.

[0028] Figure 9 This is a schematic diagram of the limiting mechanism of the present invention.

[0029] Figure 10 This is a schematic diagram of the mouse support mechanism of the present invention.

[0030] Figure 11 For the present invention Figure 1 Enlarged view of the structure at point B.

[0031] Figure 12 For the present invention Figure 4 Enlarged view of the structure at point C.

[0032] The attached figures are labeled as follows: 1. Operating platform; 2. Limiting mechanism; 21. U-shaped bracket; 22. Support leg; 23. Limiting groove; 24. Locking hole; 3. Mouse support mechanism; 31. Mouse tray; 32. Tray leg support; 33. Connecting wing plate; 34. Straight groove hole; 35. Restraint strap fixing groove; 4. Fiber optic clamping mechanism; 41. Bracket mounting base; 42. Damping pivot; 43. First support rod; 44. Lower bracket; 45. Second support rod; 46. Upper bracket; 47. Vertical threaded rod; 48. Vertical slider; 49. Horizontal guide rod; 410. Dial; 4101. Disc; 4102. Connecting slider; 4103. Angle indicator scale; 4104. Connecting shaft; 411. Fiber optic clamp; 4111. Connecting sleeve; 4112. Fiber optic fixer; 4113. Angle pointer; 5. Mouse head restraint mechanism; 51. Fixed base; 52. Support guide rod; 53. Ear rod connector; 54. Limiting ear rod; 6. Scalp spreading mechanism; 61. Guide support frame; 62. Guide slider; 63. Support sleeve; 64. Scalp traction rod; 65. Height adjuster. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] like Figure 1 - Figure 12As shown, the present invention provides a bilateral optical fiber implantation device for mouse brain, including an operating platform 1, and further including an optical fiber clamping mechanism 4 and a mouse head restraint mechanism 5 installed on the operating platform 1. The optical fiber clamping mechanism 4 is used to clamp and fix the optical fiber, and the mouse head restraint mechanism 5 is used to fix the head of the mouse. The optical fiber clamping mechanism 4 includes: a lifting platform installed on the operating platform 1; a vertical slider 48 disposed on the lifting platform, the lifting platform being connected to the vertical slider 48 via a driving component, the vertical slider 48 being configured to move along the vertical direction of the lifting platform; a horizontal guide rod 49 installed on the vertical slider 48, the outer surface of the horizontal guide rod 49 being marked; two scales 410 symmetrically disposed on the horizontal guide rod 49; and two optical fiber clamps 411 mounted on the corresponding two scales 410 via a connecting shaft 4104, the optical fiber clamps 411 being used to clamp the optical fiber.

[0035] In the above technical solution, the device is based on the principle of mechanical cooperative positioning and parameter quantification control, and uses a hierarchical structure to achieve precise implantation of bilateral optical fibers. First, the mouse head restraint mechanism 5 serves as the positioning reference layer, establishing a stable spatial coordinate system by fixing the mouse's head, providing a reference for subsequent optical fiber positioning. Second, the lifting platform and the vertical slider 48 constitute a depth adjustment layer, and the driving component converts the rotational motion into the vertical linear motion of the vertical slider 48, driving the horizontal guide rod 49 and the optical fiber to move up and down, thereby achieving precise control of the optical fiber implantation depth. Furthermore, the horizontal guide rod 49 and the symmetrical scale 410 form a symmetrical positioning and angle adjustment system. The markings on the horizontal guide rod 49 convert the horizontal spacing of the optical fibers on both sides into quantifiable values, ensuring the symmetrical distribution of the dial 410. The dial 410 is connected to the optical fiber clamp 411 via the connecting shaft 4104. With the help of the angle indicator scale 4103 and the angle pointer 4113, the tilt angle of the optical fiber can be converted into a visual scale, enabling precise angle adjustment and locking with fasteners. Finally, the optical fiber clamp 411, as the clamping execution layer, is rigidly connected to the dial 410 via the connecting shaft 4104, stably clamping the optical fiber to prevent displacement, and quantifying and controlling the key parameters of the optical fiber burial depth, horizontal spacing, and tilt angle.

[0036] Specifically, the mouse head restraint mechanism 5 provides a stable fixation benchmark for the mouse head, preventing head movement from affecting target positioning; the lifting platform and vertical slider 48 work together to precisely adjust the vertical implantation depth of the optical fiber, adapting to the depth requirements of different brain region target points; the markings on the outer surface of the horizontal guide rod 49 quantify the horizontal spacing of the bilateral scales 410, and combined with the symmetrically arranged scales 410 and optical fiber clamps 411, it can ensure that the optical fibers on both sides are strictly symmetrical in horizontal position and tilt angle, improving the accuracy of the implantation position; at the same time, the optical fiber clamps 411 are fixed to the scales 410 through the connecting shaft 4104, which can stably hold the optical fiber during implantation, eliminating the need for pre-fixation with dental cement, avoiding cement interference with subsequent implantation points, improving the convenience of implanting optical fibers at close points, and the overall structure can adapt to mice of different sizes and implantation needs of different brain regions. This implantation device can solve the problems of dental cement overflow interfering with close target points, easy displacement of pre-implanted optical fibers, and low positioning accuracy in bilateral optical fiber implantation.

[0037] The lifting platform includes: a bracket mounting base 41, mounted on the operating platform 1; and a damping shaft 42, rotatably mounted on the bracket mounting base 41. The damping shaft 42 is provided with a first support rod 43 and a second support rod 45 that are parallel to each other. The first support rod 43 and the second support rod 45 form a slide rail. A vertical slider 48 is connected to the slide rail. The bottom and top of the slide rail are respectively provided with a lower bracket 44 and an upper bracket 46 for connecting the two ends of the first support rod 43 and the second support rod 45. The lower end of the first support rod 43 passes through the lower bracket 44 and is connected to the damping shaft 42.

[0038] In the above technical solution, the lifting platform uses the bracket mounting base 41 as the basic load-bearing component, which is rigidly fixed to the operating platform 1 through the connecting parts, providing a stable reference for the subsequent adjustment structure; the damping shaft 42 adopts a rotary connection structure with friction damping, its inner ring is fixed to the bracket mounting base 41, and its outer ring is rigidly connected to the lower end of the first support rod 43. Utilizing the friction force generated by the damping component, the shaft can stop and maintain its position at any angle, realizing stepless adjustment of the slide rail angle to meet different implantation angle requirements. The damping component uses rubber gaskets, but friction plates can also be used; the first support rod 43 and the second support rod 45 are connected by... The lower bracket 44 and the upper bracket 46 form a closed rectangular slide rail frame, which not only avoids deformation of a single support rod under stress, but also provides symmetrical guiding constraints for the vertical slider 48, so that the vertical slider 48 can only move along the axis of the support rod. The lower bracket 44 and the upper bracket 46 not only serve to connect the support rod, but also limit the movement range of the vertical slider 48, preventing the slider from moving excessively and causing the optical fiber to exceed the safe implantation depth. At the same time, they enhance the overall structural strength of the slide rail, ensuring that the slide rail will not shake due to force when the drive component drives the vertical slider 48, and ultimately achieve precise adjustment of the optical fiber under the premise of adjustable angle.

[0039] The bracket mounting base 41 provides a stable bottom support for the entire lifting structure, preventing the optical fiber positioning accuracy from being affected by base swaying during operation. The damping shaft 42 can drive the first support rod 43, the second support rod 45, and the slide rail to rotate around the bracket mounting base 41, flexibly adjusting the angle between the slide rail and the mouse head restraint mechanism 5, and changing the tilt direction of the horizontal guide rod 49 and the optical fiber. This can adapt to the differences in head contours of mice of different sizes and meet the special requirements of different brain regions for optical fiber implantation angles. For example, some deep brain regions require tilted implantation to avoid blood vessels, and the damping characteristics... It can keep the adjusted angle stable without the need for additional locking components; the parallel slide rail composed of the first support rod 43 and the second support rod 45 can provide bidirectional guidance for the vertical slider 48, ensuring that the vertical slider 48 moves smoothly in the vertical direction and avoiding slider deviation caused by unilateral support. This ensures that the optical fiber is always aligned with the target point during the depth adjustment process. The connection and fixation of the two ends of the support rod by the lower bracket 44 and the upper bracket 46 can enhance the overall rigidity of the slide rail, prevent the support rod from deforming during long-term use, and improve the straightness of the vertical slider 48 movement and the stability of optical fiber implantation.

[0040] The driving component includes a vertical threaded rod 47 that passes through the upper bracket 46. The lower end of the vertical threaded rod 47 extends between the first support rod 43 and the second support rod 45. The upper end of the vertical threaded rod 47 is connected to a knob for rotation. Rotating the knob can drive the vertical slider 48 to move along the vertical direction of the lifting platform through the vertical threaded rod 47.

[0041] In the above technical solution, rotational motion is converted into linear motion through the threaded engagement of the vertical threaded rod 47 and the vertical slider 48. The vertical threaded rod 47 and the upper bracket 46 are connected by threads, and the upper bracket 46 has a pre-set internal threaded hole that matches the threaded rod, which not only provides axial support for the threaded rod but also restricts its radial sway, ensuring that the threaded rod can only rotate around its own axis. The vertical slider 48 has an internal threaded hole that matches the vertical threaded rod 47. The lower end of the threaded rod extends between the first support rod 43 and the second support rod 45 and engages with the slider threadedly. When the upper knob is rotated, the knob drives the threaded rod to rotate synchronously. Under the action of the threaded pair, the rotational motion is converted into linear motion. The vertical slider 48 moves in a straight line along the axis of the threaded rod. The parallel slide rail formed by the first support rod 43 and the second support rod 45 provides bidirectional guidance and constraint for the vertical slider 48, preventing the slider from rotating with the threaded rod or shifting laterally, and ensuring that the slider moves only in the vertical direction. In addition, the pitch of the threaded rod is designed to be minute, so that each rotation of the knob corresponds to a fixed minute displacement of the slider, realizing micro-adjustment of the depth and accurately matching the depth requirements of the target point in the mouse brain region. At the same time, the upper bracket 46 and the lower bracket 44 limit the upper and lower limit positions of the threaded rod respectively, preventing the slider from moving excessively beyond the safe range, and finally achieving controllable and precise adjustment of the fiber optic implantation depth.

[0042] Specifically, the drive component provides a precise, stable, and easy-to-operate vertical driving force for the vertical slider 48, effectively ensuring precise control of the fiber optic implantation depth and meeting the needs of brain target points for microscale depth adjustment. The structural design of the vertical threaded rod 47 penetrating the upper bracket 46 allows for radial limiting of the threaded rod by the upper bracket 46, preventing lateral displacement during rotation and ensuring that the driving force is always transmitted vertically. The knob at the upper end increases the operating torque, allowing the experimenter to easily control the adjustment speed and amplitude by manually rotating the knob. At the same time, the threaded drive has a natural self-locking characteristic. After adjustment to the target depth, the vertical slider 48 can maintain a stable position under the action of thread friction, preventing depth displacement caused by gravity or slight vibration without additional locking components. Combined with the guide rail composed of the first support rod 43 and the second support rod 45, it ensures the straightness of the vertical slider 48's movement, preventing the fiber optic from deviating from the target point due to slider tilting, and improving the accuracy and stability of fiber optic implantation depth.

[0043] The dial 410 includes: a connecting slider 4102, slidably connected to the outer surface of the horizontal guide rod 49, the connecting slider 4102 being locked to the surface of the horizontal guide rod 49 by a self-locking component; a disc 4101, mounted on the connecting slider 4102, one side of the disc 4101 being provided with angle indicating scales 4103 distributed in a circumferential array, the connecting shaft 4104 being mounted on the axis of the disc 4101; and a connecting sleeve 4111, one end of which is mounted on the fiber optic clamp 411, and the other end of which rotates... The connecting sleeve 4111 is mounted on the connecting shaft 4104, and fasteners are provided on the side of the connecting sleeve 4111 to limit the angle of the connecting sleeve 4111; the fiber optic retainer 4112 is installed at the end of the connecting sleeve 4111 away from the disc 4101, and the fiber optic retainer 4112 is used to clamp and fix the fiber optic cable; the angle pointer 4113 is installed on the outer surface of the connecting sleeve 4111, the angle pointer 4113 is attached to the disc 4101, and is adapted to the angle indication scale 4103.

[0044] In the above technical solution, a sliding adjustment pair is formed by connecting slider 4102 and horizontal guide rod 49. The inner hole of slider and the outer surface of guide rod are precisely matched to ensure smooth sliding without jamming. The self-locking component achieves position locking by the friction force generated by radially pressing the surface of guide rod. Its locking force can be intuitively controlled by the torque of the knob. The self-locking component can be a set screw with a knob. The angle scale on the disc 4101 provides a precise reference for angle adjustment. The connecting sleeve 4111 and connecting shaft 4104 form a rotary pair. The angle pointer 4113 is rigidly connected to the connecting sleeve 4111. When rotating synchronously with the sleeve, it indicates the scale of disc 4101 in real time, realizing visual feedback of angle adjustment. The fastener restricts the rotation of the sleeve by the friction force generated by axially pressing the surface of connecting shaft 4104. Its locking force can be controlled by torque to ensure that there is no angle slippage and no damage to the shaft.

[0045] It also includes: a limiting mechanism 2, installed on the operating platform 1; a mouse support mechanism 3, installed on the operating platform 1, wherein the limiting mechanism 2 is used to support and limit the mouse support mechanism 3; and a scalp spreading mechanism 6, installed on the limiting mechanism 2, used to support the mouse scalp.

[0046] The limiting mechanism 2 is a U-shaped bracket 21. The mouse support mechanism 3 is embedded in the U-shaped bracket 21. The mouse head restraint mechanism 5 and the scalp spreading mechanism 6 are both installed on the top of the U-shaped bracket 21. The closed part of the U-shaped bracket 21 faces the fiber optic clamping mechanism 4. The bottom of the U-shaped bracket 21 is provided with a support leg 22, which is connected to the operating platform 1. The top of the U-shaped bracket 21 is provided with a limiting groove 23, and the bottom wall of the limiting groove 23 is provided with a locking hole 24.

[0047] The mouse support mechanism 3 is a mouse tray 31, which is located in the middle of the U-shaped support 21. The bottom of the mouse tray 31 is provided with tray leg supports 32, so that the mouse tray 31 and the U-shaped support 21 are on the same plane. A connecting wing plate 33 extends from the side of the mouse tray 31 near the limiting groove 23. The connecting wing plate 33 is embedded in the limiting groove 23. A straight groove hole 34 is opened on the connecting wing plate 33. The straight groove hole 34 corresponds to the locking hole 24. A bolt is provided in the straight groove hole 34. The connecting wing plate 33 is connected to the U-shaped support 21 by the bolt. Several restraint strap fixing grooves 35 are symmetrically provided on the walls on both sides of the mouse tray 31 for the restraint strap to pass through.

[0048] The mouse head restraint mechanism 5 is a fixed seat 51, which is set on the top of the U-shaped bracket 21. A support guide rod 52 is vertically arranged on the top of the fixed seat 51. An ear rod connector 53 that can slide up and down is arranged on the support guide rod 52. A limiting ear rod 54 is arranged on the ear rod connector 53 for fixing the mouse head.

[0049] The scalp spreading mechanism 6 is a guide support frame 61, which is located on top of the U-shaped bracket 21. The guide support frame 61 has a slide rail inside, in which a guide slider 62 is installed. One end of the guide slider 62 extends to the outside of the guide support frame 61 and is provided with a support sleeve 63. A horizontally adjustable scalp traction rod 64 passes through the support sleeve 63. One end of the scalp traction rod 64 is configured as a hook. A height adjuster 65 is vertically installed on the top of the guide support frame 61. The lower end of the height adjuster 65 passes through the guide slider 62. Rotating the height adjuster 65 can drive the guide slider 62 to move up and down along the guide support frame 61.

[0050] In the above technical solution, the U-shaped bracket 21 is made of high-strength metal, and the support leg 22 is rigidly connected to the operating platform 1 by bolts to ensure that the whole is not shaking. Its closed opening faces the fiber optic clamping mechanism 4 so that after the mouse head is fixed, the target point and the fiber optic implantation path are in the same straight line. The limiting groove 23 and the locking hole 24 provide a reference for the sliding adjustment and locking of the mouse tray 31. The connecting wing plate 33 of the mouse tray 31 is fitted into the limiting groove 23 of the U-shaped bracket 21. The cooperation between the straight groove hole 34 and the locking hole 24 allows the tray to slide horizontally along the limiting groove 23. After the bolt is tightened, the position is locked by friction. The height of the tray leg support 32 is designed to be flush with the top surface of the U-shaped bracket 21 to prevent the mouse body from tilting due to the height difference. The restraint strap fixing groove 35 restricts the movement of the mouse trunk by means of elastic restraint straps. The fixing seat 51 of the mouse head restraint mechanism 5 is connected to the U-shaped bracket 21. 1. Rigid connection: The support guide rod 52 provides vertical guidance for the ear rod connector 53. The ear rod connector 53 can be locked in height by a set screw. The limiting ear rod 54 adopts a blunt design to avoid damaging the mouse's ear. The head is fixed by clamping the mouse's external auditory canal with the ear rods on both sides. The guide support frame 61 of the scalp opening mechanism 6 provides vertical sliding constraint for the guide slider 62 through the internal slide. The height adjuster 65 adopts a screw drive structure. When the adjuster is rotated, the rotational motion is converted into the linear displacement of the guide slider 62. The support sleeve 63 and the scalp traction rod 64 are in clearance fit, allowing the traction rod to be adjusted horizontally to adapt to different head widths. The traction rod hook adopts a smooth arc design. The scalp is opened by the reverse pulling force of the traction rods on both sides. All mechanisms use the U-shaped bracket 21 as the positioning reference to ensure spatial coordination with the fiber optic clamping mechanism 4 and ensure the accuracy of fiber optic implantation.

[0051] Specifically, the U-shaped bracket 21 serves as the core support benchmark, rigidly connected to the operating platform 1 via support legs 22. It provides a unified and stable mounting carrier for the mouse support, head restraint, and scalp spreading mechanism 6. Its closed-end orientation towards the fiber optic clamping mechanism 4 ensures precise alignment of the mouse's head with the fiber optic implantation path, preventing operational deviations. The mouse tray 31, through the sliding engagement and bolt locking of the connecting wing plate 33 and the U-shaped bracket 21 limiting groove 23, can be horizontally adjusted to accommodate mice of different sizes. The tray leg supports 32 ensure that the tray and U-shaped bracket 21 are coplanar, preventing the mouse's body from tilting. Restraint strap fixing grooves 35 on both sides allow restraint straps to be threaded through to secure the mouse's torso, preventing... During the experiment, body twisting affected head positioning; the limiting ear rod 54 of the mouse head restraint mechanism 5 slides up and down along the support guide rod 52 through the ear rod connector 53, which can accurately adapt to different mouse head heights, achieve rigid head fixation, and prevent target point displacement caused by head shaking during fiber optic implantation; the scalp traction rod 64 of the scalp opening mechanism 6 hooks the mouse scalp with a hook, and the guide slider 62 moves up and down along the guide support frame 61 with the height adjuster 65, which can adjust the opening height. The support sleeve 63 allows the traction rod to be finely adjusted horizontally to adapt to different head widths, effectively opening the scalp and maintaining stability, clearly exposing the skull surgical area, and reducing the interference of scalp obstruction on fiber optic implantation operation.

[0052] The following is a brief introduction to the operating principle and method of the bilateral fiber optic implantation device for the mouse brain proposed in this application: The experimental mice were anesthetized by inhalation of isoflurane. After they lost consciousness, the hair on their heads was removed and the skin on their heads was cleaned with saline. The connecting wing plate 33 of the mouse tray 31 was embedded into the limiting groove 23 of the U-shaped bracket 21. The tray was slid along the straight groove hole 34 to the appropriate position according to the length of the mouse body, and the fastening bolt was tightened to lock it so that the tray and the U-shaped bracket 21 were coplanar. The anesthetized mouse was placed on the mouse tray 31 with the mouse head facing the closed end of the U-shaped bracket 21. The elastic restraint band was passed through the restraint band fixing groove 35 on both sides of the tray and wrapped around the mouse torso to complete the torso restraint.

[0053] Install the fixed seat 51 of the mouse head restraint mechanism 5 on the top of the U-shaped bracket 21, slide the ear rod connector 53 up and down along the support guide rod 52 so that the limiting ear rod 54 is aligned with the mouse's bilateral external ear canals, push the ear rod until it fits against the inner wall of the external ear canal, tighten the set screw to lock the height of the ear rod connector 53, ensure that the mouse's head does not sway left and right, and establish a stable spatial positioning benchmark; if it is necessary to adjust the head pitch angle, slowly rotate the frame to the target angle through the L-shaped support rod damping pivot 42 on the back of the mouse head restraint mechanism 5. The damping characteristics can keep the angle stable without additional locking.

[0054] Fix the guide support frame 61 to the top of the U-shaped bracket 21, ensuring it is directly above the mouse's head; rotate the height adjuster 65 to drive the guide slider 62 down along the slide, so that the support sleeve 63 is lowered to a position close to the scalp; then hook the hooks of the two scalp traction rods 64 onto the scalp on both sides of the mouse's head, and finely adjust the distance between the traction rods horizontally along the support sleeve 63 to make the scalp symmetrically spread; rotate the height adjuster 65 again to drive the guide slider 62 up until the skull is completely exposed and there is no scalp obstruction, tighten the set screw to lock the position of the guide slider 62.

[0055] Two optical fibers to be implanted are respectively inserted into the fiber optic retainers 4112 of the optical fiber clamps 411 on both sides. The clamping force of the retainers is adjusted to complete the initial fixation of the optical fibers. Then, the connecting slider 4102 of the scale 410 is pushed to slide along the horizontal guide rod 49. Referring to the markings on the outer surface of the horizontal guide rod 49, the horizontal distance between the two scales 410 is adjusted so that the axes of the two optical fibers are aligned with the line connecting the two target points marked on the skull. The set screw is tightened to lock the position of the connecting slider 4102 to ensure horizontal symmetry. Then, the angle pointer 4113 on the connecting sleeve 4111 and the scale of the disc 4101 are observed. The connecting sleeve 4111 is rotated to adjust the optical fiber to the preset implantation angle. After the angle pointer 4113 is aligned with the target scale on the disc 4101, the fastener is tightened to restrict the rotation of the connecting sleeve 4111 and complete the angle locking.

[0056] According to the target implantation angle requirements, the damping shaft 42 of the lifting platform is rotated, which drives the slide rail composed of the first support rod 43 and the second support rod 45 to rotate as a whole, so that the tilt direction of the horizontal guide rod 49 and the optical fiber is consistent with the preset implantation angle. The damping component can keep the slide rail angle stable without additional locking. Then, the knob of the drive component is rotated clockwise, which drives the vertical threaded rod 47 to rotate around its own axis. Through the threaded pair transmission, the vertical slider 48 is pushed vertically downward along the first support rod 43 and the second support rod 45. The optical fiber moves closer to the skull target point with the vertical slider 48. Then, referring to the pitch of the vertical threaded rod 47 and the depth requirements of the brain region target point, the number of rotations of the knob is controlled so that the tip of the optical fiber is gradually implanted to the target depth. The self-locking characteristic of the threaded transmission can ensure that the vertical slider 48 stays in the target position without depth deviation caused by gravity or vibration, thus completing the bilateral optical fiber deep implantation.

[0057] By observing under a microscope or performing a Micro-CT scan, confirm whether the position and depth of the optical fibers on both sides meet the requirements. If there is a deviation, the depth can be finely adjusted by rotating the knob in the opposite direction, or the angle can be finely adjusted by loosening the dial 410 fastener.

[0058] In summary, compared with the prior art, the bilateral fiber optic implantation device for the mouse brain proposed in this application has the following advantages: The fiber optic clamp 4112 can directly and rigidly hold the fiber optic cable. With the self-locking component of the connecting slider 4102 of the dial 410 and the fastener of the connecting sleeve 4111, the horizontal position and tilt angle of the fiber optic cable can be locked respectively, ensuring that the fiber optic cable maintains a stable posture throughout the implantation process. At the same time, the marking on the outer surface of the horizontal guide rod 49 and the symmetrical dial 410 design can precisely control the horizontal distance between the two fiber optic cables. Even if the two target points are close to each other, the positioning boundaries of the two fiber optic cables can be clearly distinguished by quantitative adjustment, avoiding target point confusion caused by cement overflow. The scalp opening mechanism 6 symmetrically opens the scalp through the traction rod, clearly exposing the skull target area and further reducing operation obstruction.

[0059] The damping shaft 42 of the lifting platform can stably adjust the slide rail angle. The parallel slide rail composed of the first support rod 43 and the second support rod 45 provides bidirectional guidance for the vertical slider 48, ensuring that the optical fiber is always aligned with the target point when it is tilted during implantation. The vertical threaded rod 47 of the drive component realizes the micro-displacement adjustment of the vertical slider 48 through threaded transmission. The threaded structure has a self-locking characteristic, and no additional locking is required after adjustment to the target depth, which can prevent the optical fiber from being deviated due to gravity or vibration. The limiting ear rod 54 and the supporting guide rod 52 of the mouse head restraint mechanism 5 are adapted to different mouse head heights to achieve rigid head fixation and avoid target point displacement caused by head shaking during fiber implantation. The tray of the mouse support mechanism 3 can be adjusted horizontally along the limiting groove 23 of the U-shaped bracket 21, and together with the restraint strap, it fixes the mouse torso to prevent body twisting from interfering with head positioning, improves the positioning accuracy of bilateral fiber optics, and adapts to the needs of different mouse sizes and different brain regions for implantation. With stepless angle adjustment of the lifting platform, knob operation of the drive component, and visual angle indication on the dial 410, the operation is intuitive and the adjustment force can be sensed in real time. The quick locking of the mouse tray 31 and the height and horizontal fine adjustment function of the scalp spreading mechanism 6 simplify the experimental operation steps and improve the operation efficiency and repeatability of bilateral fiber optic embedding.

[0060] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0061] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A bilateral fiber optic implantation device for mouse brain, comprising an operating platform (1), characterized in that, It also includes an optical fiber clamping mechanism (4) and a mouse head restraint mechanism (5) installed on the operating platform (1). The optical fiber clamping mechanism (4) is used to clamp and fix the optical fiber, and the mouse head restraint mechanism (5) is used to fix the head of the mouse. The fiber optic clamping mechanism (4) includes: A lifting platform is installed on the operating platform (1); A vertical slider (48) is set on the lifting platform. The lifting platform is connected to the vertical slider (48) through a drive component. The vertical slider (48) is set to move along the vertical direction of the lifting platform. A horizontal guide rod (49) is mounted on a vertical slider (48), and the outer surface of the horizontal guide rod (49) is marked. Two dials (410) are symmetrically arranged on the horizontal guide rod (49); Two fiber optic clamps (411) are mounted on two corresponding dials (410) via a connecting shaft (4104). The fiber optic clamps (411) are used to hold the fiber optic cable.

2. The bilateral fiber optic implantation device for the mouse brain according to claim 1, characterized in that: The lifting platform includes: The bracket mounting base (41) is installed on the operating platform (1); The damping shaft (42) is rotatably mounted on the bracket mounting base (41); The damping shaft (42) is provided with a first support rod (43) and a second support rod (45) that are parallel to each other. The first support rod (43) and the second support rod (45) form a slide rail. The vertical slider (48) is connected to the slide rail. The bottom and top of the slide rail are respectively provided with a lower bracket (44) and an upper bracket (46) for connecting the two ends of the first support rod (43) and the second support rod (45). The lower end of the first support rod (43) passes through the lower bracket (44) and is connected to the damping shaft (42).

3. The bilateral fiber optic implantation device for the mouse brain according to claim 2, characterized in that: The driving component includes: A vertical threaded rod (47) passes through the upper bracket (46). The lower end of the vertical threaded rod (47) extends between the first support rod (43) and the second support rod (45). The upper end of the vertical threaded rod (47) is connected to a knob for rotation. Rotating the knob can drive the vertical slider (48) to move along the vertical direction of the lifting platform through the vertical threaded rod (47).

4. The bilateral fiber optic implantation device for the mouse brain according to claim 1, characterized in that: The dial (410) includes: The connecting slider (4102) is slidably connected to the outer surface of the horizontal guide rod (49), and the connecting slider (4102) is locked to the surface of the horizontal guide rod (49) by a self-locking component; A disc (4101) is mounted on a connecting slider (4102). An angle indicator scale (4103) is arranged in a circular array on one side of the disc (4101). The connecting shaft (4104) is mounted on the axis of the disc (4101). The connecting sleeve (4111) is mounted on the fiber optic clamp (411) at one end and rotated on the connecting shaft (4104) at the other end. Fasteners are provided on the side of the connecting sleeve (4111) to limit the angle of the connecting sleeve (4111). A fiber optic clamp (4112) is installed at the end of the connecting sleeve (4111) away from the disc (4101). The fiber optic clamp (4112) is used to clamp and fix the fiber optic cable. An angle pointer (4113) is mounted on the outer surface of the connecting sleeve (4111). The angle pointer (4113) is attached to the disc (4101) and is adapted to the angle indication scale (4103).

5. The bilateral fiber optic implantation device for the mouse brain according to claim 1, characterized in that: Also includes: Limiting mechanism (2) is installed on operating platform (1); A mouse support mechanism (3) is installed on the operating platform (1), and the limiting mechanism (2) is used to support and limit the mouse support mechanism (3); The scalp stretching mechanism (6) is installed on the limiting mechanism (2) and is used to support the mouse scalp.

6. The bilateral fiber optic implantation device for the mouse brain according to claim 5, characterized in that: The limiting mechanism (2) is a U-shaped bracket (21). The mouse support mechanism (3) is embedded in the U-shaped bracket (21). The mouse head restraint mechanism (5) and the scalp spreading mechanism (6) are both installed on the top of the U-shaped bracket (21). The closed part of the U-shaped bracket (21) faces the fiber optic clamping mechanism (4). The bottom of the U-shaped bracket (21) is provided with a support leg (22), which is connected to the operating platform (1) through the support leg (22). The top of the U-shaped bracket (21) is provided with a limiting groove (23), and the bottom wall of the limiting groove (23) is provided with a locking hole (24).

7. The bilateral fiber optic implantation device for the mouse brain according to claim 6, characterized in that: The mouse support mechanism (3) is a mouse tray (31). The mouse tray (31) is set in the middle of the U-shaped support (21). The bottom of the mouse tray (31) is provided with a tray leg support (32) so that the mouse tray (31) and the U-shaped support (21) are on the same plane. The side of the mouse tray (31) near the limiting groove (23) extends out a connecting wing plate (33). The connecting wing plate (33) is embedded in the limiting groove (23). A straight groove hole (34) is opened on the connecting wing plate (33). The straight groove hole (34) corresponds to the locking hole (24). A bolt is provided in the straight groove hole (34). The connecting wing plate (33) is connected to the U-shaped support (21) by the bolt.

8. The bilateral fiber optic implantation device for the mouse brain according to claim 7, characterized in that: The mouse tray (31) has several restraint strap fixing grooves (35) symmetrically arranged on both sides of the wall for the restraint strap to pass through.

9. The bilateral fiber optic implantation device for the mouse brain according to claim 6, characterized in that: The mouse head restraint mechanism (5) is a fixed seat (51). The fixed seat (51) is set on the top of the U-shaped bracket (21). A support guide rod (52) is vertically set on the top of the fixed seat (51). An ear rod connector (53) that can slide up and down is set on the support guide rod (52). A limit ear rod (54) is set on the ear rod connector (53) for fixing the mouse head.

10. The bilateral fiber optic implantation device for the mouse brain according to claim 6, characterized in that: The scalp spreading mechanism (6) is a guide support frame (61). The guide support frame (61) is set on the top of the U-shaped bracket (21). The guide support frame (61) has a slide rail inside, and a guide slider (62) is set in the slide rail. One end of the guide slider (62) extends to the outside of the guide support frame (61) and is provided with a support sleeve (63). The support sleeve (63) is through which a horizontally adjustable scalp traction rod (64) passes. One end of the scalp traction rod (64) is set as a hook. A height adjuster (65) is vertically set on the top of the guide support frame (61). The lower end of the height adjuster (65) passes through the guide slider (62). Rotating the height adjuster (65) can drive the guide slider (62) to move up and down along the guide support frame (61).

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