Dedicated centrifugal tube and method for manufacturing cell blocks

A centrifuge tube and cell block technology, which is applied in the field of cytopathology, can solve the problems of the size and shape of the wax block, the inability to make a definite diagnosis of the patient, and the inability to make a cell block, so as to improve the positive rate of diagnosis and effectively It is beneficial to the quality control and standardized operation of cytopathology, and the effect of determining the location of cell distribution

Active Publication Date: 2015-10-21

昆明东环科技有限公司

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AI-Extracted Technical Summary

Problems solved by technology

However, these existing methods have different defects, the most obvious defect is: it is not suitable for the preparation of cell blocks when the number of cells or cell clusters is small, therefore, some clinical specimens cannot be made due to the small number of cells Cell-forming wax block, which cannot make a definite...

Method used

[0027] Centrifuge tube 1. openings at both ends are cylindrical hollow tubes, and the outer skins at both ends have external threads (external threads are not marked in the figure). Centrifuge tube caps ② and ③ are flat-bottomed, with a sealing ring (gasket) on the bottom of the cap (the sealing ring is not marked in the figure), and a closed type cap with internal thre...

Abstract

The invention discloses a dedicated centrifugal tube and method for manufacturing cell blocks. The dedicated centrifugal tube is composed of a cylindrical centrifugal pipe with two opened ends and two centrifugal tube covers. The surfaces of the pipe walls of two ends of the centrifugal tube are provided with external threads, the centrifugal tube covers are covers with a flat bottom, the bottoms of the covers are provided with a sealing ring (cushion), the internal walls are provided with internal threads, and the internal threads and the external threads can be engaged together to form a sealed cylindrical space. The cell block is produced by the following steps: heating a centrifugal tube containing matrix to liquidize the matrix, then adding cell (cluster) suspension on the upper surface of the matrix layer, turning the centrifugal tube upside down to mix the matrix and the cell suspension, then carrying out centrifugation, (or directly carrying out centrifugation), so as to enrich the cells to a plane on the bottom layer of the matrix through the matrix (cushion); after the matrix is cured, removing the centrifugal tube cover on the two ends of the centrifugal tube, pushing out the matrix containing a cell layer by a piston or pressurized air from the centrifugal tube, and cutting the matrix to manufacture cell blocks. The provided method can conveniently and efficiently make trace cell samples into a complete and regular cell block with a large cell distribution area and high cell density.

Application Domain

Preparing sample for investigation

Technology Topic

Push outCell layer +6

Image

Examples

Experimental program(1)

Example Embodiment

[0025] The present invention will be described in detail below in conjunction with examples and drawings, but the present invention is not limited to this.

[0026] Such as figure 1 As shown, a special centrifuge tube for preparing cell masses is composed of centrifuge tube ① and centrifuge tube covers ② and ③.

[0027] Centrifuge tube ① is a cylindrical hollow tube with open ends at both ends, with external threads on the outer surface of both ends (external threads are not shown in the figure). Centrifuge tube caps ② and ③ are flat-bottomed, with a sealing ring (pad) at the bottom of the cap (the sealing ring is not shown in the figure), and a closed cap with internal threads on the surface of the inner side wall (internal threads are not shown in the figure). The flat bottom is the structural basis for cells to be distributed on a flat surface. Centrifuge tube caps ② and ③ can be screwed into the external threads at both ends of the centrifuge tube ① to prevent liquid from seeping out and form a closed cylindrical space.

[0028] Such as figure 2 As shown, after screwing the centrifuge tube cover to both ends of the centrifuge tube, a closed cylindrical space is formed.

[0029] Such as image 3 As shown, when the diameter of the centrifuge tube is less than 5mm, in order to keep the centrifuge tube ② upright during centrifugation and operation, the centrifuge tube ② with a small diameter can be placed in the centrifuge tube ① with a large diameter and nested together. The large centrifuge tube is used as a centrifuge support for the small centrifuge tube. Upright is the state that the centrifuge tube needs to be maintained during operation and centrifugation.

[0030] Such as Figure 4 As shown, the substrate ② is pre-packed into centrifuge tubes. When making cell masses, the centrifuge tube with substrate ② is placed in the baking sheet machine at a temperature higher than the melting point of the substrate to melt the substrate ② into a liquid state. Unscrew the top of the centrifuge tube cap, add the cell suspension ① to the substrate ②, screw on (tighten) the centrifuge tube cap, mix the cell suspension ① and the liquid substrate ② upside down and centrifuge or centrifuge directly. Another method is to directly add the cell suspension ① to the solid matrix ②, heat and melt the matrix, directly centrifuge or mix and centrifuge to enrich the cells. The molecules on some cells are sensitive to heat. Increased temperature will easily destroy the molecules and cannot be detected. At this time, the matrix can be heated and melted and kept at 37°C, while the cell suspension is also heated to 37°C, then perform the above operations .

[0031] Such as Figure 5 As shown, after centrifugation, the cells are enriched on the plane of the lower layer of the matrix ② under the action of centrifugal force, forming a button-like cell layer ①. When there are few cells, it may just form a thin cell membrane①.

[0032] Such as Image 6 Shown, will Figure 5 Put the centrifuge tube upright in the refrigerator at 4°C for 15 minutes. After the matrix material has solidified, unscrew the centrifuge tube caps at both ends, and use the principle of ear-washing ball pressurized air to push the matrix end with the cell layer ① out of the centrifuge tube ( Image 6 The method of pressurizing the ear ball is not shown in). When the inner diameter of the centrifuge tube> At 5mm, use the principle of piston ③ to push out the end of the matrix with the cell layer ①, and the operation can be completed by using a cotton swab. Use a blade to cut the matrix material (thickness 2-3mm) with the cell layer ① to make a cell block with a fixed shape and cells distributed on one surface. After the cell block is processed by conventional pathological techniques, it is made into a cell wax block.

PUM

Property	Measurement	Unit
The inside diameter of	1.0 ~ 15.0	mm
Outer diameter	<= 20.0	mm
Length	60.0 ~ 80.0	mm

Description & Claims & Application Information

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Dedicated centrifugal tube and method for manufacturing cell blocks

AI-Extracted Technical Summary

Problems solved by technology

Method used

Abstract

Image

Examples

Example Embodiment

PUM

Description & Claims & Application Information

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