Method for establishing standardized tumor animal model and culture device

Abstract

The embodiment of the invention discloses a method for establishing a standardized tumor animal model and a culture device. The method comprises the following steps that a 3D cell ring culture mold ispretreated, and the pretreated 3D cell ring culture mold is placed into a cell culture dish; the 3D cell ring culture mold is obliquely rotaed in the space of the cell culture dish according to different sequences, and 3D tumor cell rings with uniformly distributed tumor cells are cultured on the 3D cell ring culture mold; and the 3D tumor cell ring is cultured in vitro until the 3D tumor cell ring is formed, the 3D tumor cell ring is transplanted into the animal tissue for secondary culture, and the tumor cells of the 3D tumor cell ring are enabled to bidirectionally grow at the modeling position of the animal tissue until the tumor grows and is formed. According to the scheme, cell loss is little, the tumor formation rate is high, tumor formation is uniform and stable, the number of thecells required by modeling and the modeling cost are greatly reduced, and the method for establishing the standardized tumor animal model is efficient, simple, reliable, high in operability, economical and practical.

Description

technical field [0001] The embodiments of the present invention relate to the field of biomedical technology, and in particular to a method for establishing a standardized tumor animal model and a culture device. Background technique [0002] Malignant tumor is the biggest killer that endangers human health. Scientists all over the world have been trying to find the best treatment method against malignant tumor. Tumor research has always been the most concerned field in the world. The current field of basic tumor research includes the study of tumor cells in vitro and the study of tumor animal models in vivo. [0003] At present, the most commonly used tumor animal model is the mouse model. The methods for establishing tumor models mainly include cell suspension injection, cell suspension gel mixed injection, and tissue block transplantation. The existing tumor animal models The shortcomings of the establishment method are as follows: [0004] The cell suspension injectio...

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Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides a method for establishing a standardized tumor animal model and a culture device to solve the shortcomings of the prior art such as uneven number of tumor cells in tissue blocks, poor stability of tumor formation, and uncontrollable consistency of tumor formation. question

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