The invention relates to the technical field of biology, in particular to a stable NSE quality control product and a preparation method thereof. The NSE quality control product provided by the invention is prepared from an NSE antigen, a protein protective agent, a preservative, an antibiotic, a freeze-drying protective agent, magnesium ions and human serum. The product can effectively weaken thedescending speed of a tumor marker NSE in a matrix liquid, has good product uniformity, long-term storage stability and stability after redissolution, is not influenced by transportation, temperatureand other factors, has long stabilization time in the detection process after redissolution, has detection repeatability and high accuracy, and can be suitable for the quantitative detection of different instrument models and different reagents. The product can be used for detecting by operators in different regions and different laboratories, can be suitable for quantitative detection kits of different brands, can meet the clinical quality control requirement on the tumor marker NSE, and can more accurately detect the accuracy of a clinical sample detection result.