The invention relates to the technical field of agriculture, in particular to preparation and application of vegetable planting soil. The vegetable planting soil is prepared from the following components including mushroom bran, earthworm fertilizer and fine river sand, wherein the ratio of the mushroom bran to the earthworm fertilizer to the fine river sand is 5:3:1; and the mushroom bran, the earthworm fertilizer and the fine river sand are placed in a fungus bag, the length of the fungus bag is 20-30 cm, and the diameter of the fungus bag is 15-20 cm. According to the preparation and application of the vegetable planting soil, vegetables are watered with clear water within 20 days after field planting, topdressing is performed once every 10-15 days after 20 days, and uniform fertilization is performed in the fungus bag 5 cm away from vegetable roots. Reasonable fertilization is carried out according to habits of the vegetables. The invention discloses the preparation and applicationof the vegetable planting soil, and relates to an innovative planting manner for vegetable growth. Land limitation is avoided, the vegetables grow fast, diseases and insect pests are few, crop rotation is easy, no soil continuous cropping obstacle exists, the quality is not changed within 365 days, territory limitation is avoided, south and north production can be achieved, the purposes of no chemical pesticide and less pesticides are achieved, and standardized technical management and control are achieved.