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A detection method and a technique for rose polyphenols are applied in the detection field of rose polyphenols and can solve problems such as comprehensive utilization of resources that have not yet been obtained.
Active Publication Date: 2021-10-08
SUZHOU QIUSHI HERBAL HEALTH TECH CO LTD
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Problems solved by technology
In industrial applications, rose polyphenols, as waste by-products in the production of rose essential oil, have not been comprehensively utilized as resources
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Embodiment 1
[0024] Example 1: HPLC characteristic spectrum analysis of rose polyphenol index components
[0025] Five kinds of rose medicinal materials were purchased from Hetian, Xinjiang (S1, double rose petals; batch MG20180901); Shandong (S2, Shandong Pingyin rose buds; batch MG20180902); Yunnan (S3, double rose petals; lot MG20180903); Anhui (S4, double rose petals; lot MG20180904) and Gansu (S5, Kushui rose petals; lot MG20180905). Ellagic acid, kaempferol, and quercetin reference substances were purchased from Dalian Meilun Biotechnology Co., Ltd., with a purity of ≥98%; chlorinated cyanidin-3-glucoside reference substances were purchased from Chengdu Effa Biotechnology Co., Ltd. company, purity ≥98%.
[0026] (1) Preparation of the test solution: Take about 1 g of dried rose powder (over a 30-mesh sieve) from five places of origin, weigh them accurately, place them in 100 ml triangular flasks with stoppers, add 50 ml of 80% methanol to each of them accurately, and weigh them. De...
Embodiment 2
[0032] Example 2: HPLC content analysis of rose polyphenol index components
[0033] Aiming at the structural characteristics of rose polyphenols and five index components, the present invention uses acid hydrolysis for the first time to establish a quantitative analysis method for the total content of rose polyphenols.
[0034] (1) Preparation of the test solution: Take about 1 g of dried rose powder (over a 30-mesh sieve) from five places of origin, weigh them accurately, place them in 100 ml triangular flasks with stoppers, add 50 ml of 80% methanol to each of them accurately, and weigh them. Determine and record the weight, ultrasonically extract for 30 minutes (power 250W, frequency 40kHz), take it out, let it cool, weigh and make up the weight; accurately measure 25ml of the extract, concentrate to dryness, wash twice with 2-3ml of petroleum ether, and concentrate The substance was dissolved in 25ml of 5% hydrochloric acid, reacted in a boiling water bath for 1.5h, allow...
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Abstract
The invention discloses a detection method of rose polyphenols. The method comprises the following steps that: a high performance liquid chromatography and an ultraviolet detector are selected to carry out characteristic spectrum analysis on rose polyphenol by using five index components, namely cyanidin-3-O-glucoside, quercetin-3-O-beta-D-sophoroside, kaempferol-3-O-beta-D-sophoroside, ellagic acid, kaempferol-3-O-(6 ''-galloyl)-beta-D-glucoside and the like; and the total content of the rose polyphenols in the ellagic acid, quercetin and kaempferol is quantitatively analyzed by adopting a sample acid hydrolysis treatment method. Based on characteristic index components in roses, a qualitative and quantitative analysis method for specificity of the roses is established, and the method has the remarkable advantages of being easy and convenient to operate and use, easy to popularize and use and the like, and can be applied to quality analysis and control of the roses, extracts of the roses and other related products.
Description
technical field [0001] The invention relates to a detection method of rose polyphenols, and also relates to the application of the detection method in quality control of Rosa flowers and their extracts. Background technique [0002] Roses and Chinese roses are plants of the genus Rosa (Rosaceae) native to my country. They have a long history of cultivation in China and are planted all over the country. Globally, roses are widely used as ornamental flowers. In addition, the international industrial use is mainly to produce essential oils. Bulgaria is currently the country with the highest rose oil production in the world. Rose is a traditional medicinal and edible material in my country. The flower is rich in various nutrients such as amino acids, proteins, polysaccharides, starch, and vitamins. There are people who eat rose flowers (petals) and drink scented tea (buds) all over our country. custom. According to the Chinese Pharmacopoeia 2020 edition, the medicinal parts of ...
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