Diagnosis quality-control cell strain for common numerical abnormalities of chromosomes and preparation method thereof
A technology for chromosome number and abnormal diagnosis, applied in biochemical equipment and methods, cells modified by introducing foreign genetic material, using vectors to introduce foreign genetic material, etc., can solve the problem of no feasible quality control substances
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[0019] 1. Primordial cells: The primitive cells used are derived from trisomy 21, trisomy 18, trisomy 13, X or Y chromosome after diagnosis of chromosomal disease due to the needs of clinical diagnosis and treatment and have been diagnosed as one of the common abnormalities in chromosome number Abnormal remaining primary or passage living tissue cells with adherent growth; the primary or passage living tissue cells may also involve living tissue cells with abnormal chromosome structure or No. 1 to No. Live tissue cells with abnormal number of chromosome 22. The cell types are fetal amniotic fluid, villous tissue and other cells.
[0020] 2. Extraction of SV40 large T antigen DNA: ① SV40 DNA digestion: buy SV40 freeze-dried powder or SV40 plasmid containing large T antigen gene from the market, dissolve in appropriate amount of H 2 In O or TE buffer, add 2uL 10× digestion buffer and 18uL H 2O, add restriction endonuclease BamH I (1-5U / ugDNA), incubate at 37°C for 1h, heat at ...
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