Preparation method and application of injectable allogeneic adipose acellular matrix particles

Abstract

The invention discloses a preparation method and application of injectable allogeneic adipose acellular matrix particles. The injectable allogeneic adipose acellular matrix particles are obtained in the modes that adipose tissue obtained through liposuction operation is subjected to mechanical chylosis treatment, centrifugal degreasing and a subsequent gentle acellular working procedure, and centrifuged for further degreasing. The adipose tissue comes from human-derived adipose particles discarded by traditional liposuction operation of obese patients. Adipose cell physical crushing and degreasing methods are adopted, a gentle acellular technology is applied, operation is easy and convenient, damage to adipose matrix structure and components is small, an obtained material is closer to thehuman body natural adipose tissue microenvironment, and the three-dimensional structure and active components of the tissue are preserved; and the injectable allogeneic adipose acellular matrix particles can be injected through a 27g of small needle, after injection, proliferation and differentiation of tissue cells in a recipient site are more facilitated, thus tissue repairing and regeneration are promoted, and the long-term filling and rejuvenation effects are achieved. The injectable allogeneic adipose acellular matrix particles are suitable for facial rejuvenation and soft tissue defect repairing.

Description

technical field [0001] The invention relates to the field of medical plastic cosmetic materials, in particular to a preparation method and application of injectable allogeneic adipose acellular matrix particles. Background technique [0002] With the development of society, the improvement of people's living standards and the popularization of micro-plastic surgery in recent years, the application of injection filling materials has greatly increased in international and domestic demand. Currently, hyaluronic acid is commonly used as an injection filling material. It has obvious advantages, but it also has the problem of degradation and absorption, and it cannot stimulate tissue regeneration, so it cannot fundamentally solve the problem of rejuvenation. [0003] Non-surgical or minimally invasive treatment is one of the development directions of plastic surgery. Filling treatment is often the first choice for facial fine wrinkles or volume loss. Filling of soft tissues of the...

AI Technical Summary

Problems solved by technology

Moreover, degreasing with various organic solvents such as isopropanol not only takes a long time, but also causes subsequent organic solvent residues, which affects the biological safety and biological activity of the matrix material.

In addition, the method for preparing microparticles in this patent is to cut large pieces of adipose tissue from animal sources into thin slices, decellularize them and then grind them at low temperature to produce injectable microparticles. The action time and concentration of the action will increase the risk of reagent residue

Reagent residues are toxic to cells. This degreasing method can destroy the natural chemical structure and composition of the cell matrix, and the biological activity of the material will decrease, which will further affect the tissue regeneration induced by the scaffold material, thus limiting its injection filling effect.