LAMP primer composite for detecting respiratory pathogens and kit of LAMP primer composite

Abstract

The invention relates to a primer composite for detecting respiratory pathogens. The primer composite comprises at least one group of a mycoplasma pneumoniae group, a chlamydia pneumoniae primer group, an influenza A / B virus primer group, a parainfluenza virus primer group, an adenovirus primer group and a respiratory syncytial virus primer group. The invention further relates to a kit comprising the primer composite. The kit further comprises a micro-fluidic chip wrapping primers, and a macroscopic indicator. The invention further relates to a detection method adopting the primer composite. The detection method comprises the steps of primer composite coating, to-be-detected sample nucleic acid extraction, LAMP reaction and visual result interpretation. The kit and the method are applied to the micro-fluidic chip for visual judgment, instant detection of the seven respiratory pathogens is rapidly and accurately realized, and a result is judged by a naked eye by color change, so that the kit and the method are simpler, more convenient and quicker in practical applications, are easy to operate, and are suitable for site operation.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid amplification, and in particular relates to a LAMP primer composition for detecting respiratory pathogens, a kit for detecting respiratory pathogens based on a microfluidic chip system and a method thereof. Background technique [0002] Respiratory tract infection is a common infectious disease in my country, which refers to pathogens infecting the respiratory system such as the nasal cavity, throat, trachea and bronchi of the human body. Upper respiratory tract infection is mainly caused by viruses, which refers to inflammation of the nasal cavity, pharynx or larynx. The most common is acute upper respiratory tract infection, which is highly contagious and can cause serious complications. Lower respiratory tract infections are caused by microorganisms such as viruses, bacteria, mycoplasma, and chlamydia. Lower respiratory tract infections include bronchitis, pneumonia, etc. If the pathogen c...

AI Technical Summary

Problems solved by technology

Among them, the indirect immunofluorescence method requires the help of a fluorescent microscope, and the judgment results require experienced testing personnel; although colloidal gold detection is fast, its sensitivity is not high, and false negative results are prone to occur

The specificity and sensitivity of enzyme-linked immunoassay are not high, and the operation process is cumbersome, time-consuming and laborious

Fluorescent PCR method can detect pathogenic nucleic acid more accurately, but multiple fluorescent PCR products can detect up to 3 targets, the detection time is long, the detection system and operation process are relatively complicated, it is difficult to carry out on-site detection, and professional personnel are required to operate specific detection instruments, etc.

[0008] At present, many studies combine loop-mediated constant temperature amplification with microfluidic chips to quickly detect pathogenic nucleic acids, tumor marker genes, drug resistance sites, etc., but the above studies need to use other instruments to judge the results

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