Minimum promoter for enhancing activity and expression of exogenous protein in bacteria
A technology of exogenous proteins and promoters, applied in biochemical equipment and methods, microbial assays/tests, resistance to vector-borne diseases, etc. Problems such as being detected to achieve the effect of enhancing expression and activity
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[0025] A minimal promoter that enhances the activity and expression of foreign proteins in bacteria is 265 nucleotides upstream of the coding region of the SspH1 gene of Salmonella enterica subsp. 1.
[0026] Identification of a minimal promoter that enhances expression of foreign proteins in bacteria:
[0027] 1. Clone the SspH1 gene promoter of Salmonella enterica subspecies Chlamydia enterica typhimurium strain RM13672;
[0028] Primers were designed according to the sequence 615 nucleotides in length upstream of the SspH1 coding region in the chromosomal sequence of Salmonella enterica subsp. Use the primer pair pSspH1-615-F / pSspH1-R1, bacterial genomic DNA and high-fidelity Tag DNA polymerase to establish a polymerase chain reaction (PCR). The reaction conditions are: 95°C for 2min, then 35 cycles of 95°C for 30sec , 55°C 30sec, 72°C 1min; finally 72°C 5min. The 0.6 kb DNA band generated by PCR was purified by agarose gel electrophoresis.
[0029] The primer sequence...
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